DNA Extraction and Purification Quiz

A test used to confirm the presence of DNA in a sample.

A chemical indicator used in the Dische test that reacts with deoxyribose sugar, producing a blue color.

This sugar is specifically required for a positive result in the Dische test for DNA.

This method involves hydrolysis of DNA in an acidic environment to release deoxyribose.

A specific compound produced in the Dische test when deoxyribose reacts with diphenylamine, indicating the presence of DNA.

The process of breaking down DNA into smaller fragments using enzymes.

The absorbance of light at a specific wavelength. In the context of DNA, it refers to the amount of light absorbed by DNA at 260 nm.

The ratio of absorbance at 260 nm to 280 nm, used to assess the purity of a DNA sample. A ratio close to 1.8 indicates a relatively pure DNA sample.

This ratio indicates a sample has relatively little contamination from protein.

The process of measuring the concentration and purity of DNA. It involves measuring the absorbance at 260 nm and 280 nm.

It absorbs UV light at specific wavelengths, providing a basis for measuring its concentration and purity.

A measure of how much light is absorbed by a substance at a specific wavelength. It reflects DNA concentration.

This indicates that a sample is free from protein and other contaminants.

A pure sample of DNA has a 260/280 ratio of around 1.8, indicating high purity and minimal contamination.