Eukaryotic Animal Cells and Tissue Preparation
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Questions and Answers

What is the primary function of albumin in blood?

  • To maintain osmotic pressure (correct)
  • To provide energy
  • To facilitate clotting
  • To transport oxygen
  • Where does hemopoiesis primarily occur during the second trimester?

  • In the spleen
  • In the bone marrow
  • In the liver (correct)
  • In the lungs
  • Which statement about leukocytes is correct?

  • They require plasma proteins for function
  • They are spherical in blood and can become amoeboid after leaving blood vessels (correct)
  • They are all motile in blood
  • They have a longer lifespan than erythrocytes
  • What type of cartilage contains elastic fibers in addition to collagen type II?

    <p>Elastic cartilage</p> Signup and view all the answers

    Which type of bone marrow is filled with adipocytes and excludes hemopoietic cells?

    <p>Yellow bone marrow</p> Signup and view all the answers

    How do neutrophils function in the immune response?

    <p>They are active phagocytes of bacteria</p> Signup and view all the answers

    Which of the following components comprises the extracellular matrix of cartilage?

    <p>Collagen and glycosaminoglycans</p> Signup and view all the answers

    What happens to yellow bone marrow under severe bleeding or hypoxia?

    <p>It transforms into red marrow</p> Signup and view all the answers

    What component of blood is involved in the initial response to form a clot?

    <p>Fibrinogen</p> Signup and view all the answers

    What is a common shape of erythrocytes that aids in their function?

    <p>Biconcave</p> Signup and view all the answers

    What is the primary function of osteocytes in bone tissue?

    <p>To regulate and maintain the mineralized matrix</p> Signup and view all the answers

    What shape do osteoblasts adopt when they are actively synthesizing bone matrix?

    <p>Cuboidal or columnar</p> Signup and view all the answers

    What type of collagen makes up the organic components of bone?

    <p>Type I collagen</p> Signup and view all the answers

    What is a key characteristic of osteoclasts?

    <p>They are large, motile cells with multiple nuclei</p> Signup and view all the answers

    What role does the periosteum play in bone structure?

    <p>Nourishes the osseous tissue</p> Signup and view all the answers

    How do osteocytes communicate with one another?

    <p>Using gap junctions on dendritic processes</p> Signup and view all the answers

    What substance is primarily involved in the resorption process of bony tissue?

    <p>Enzymatic material</p> Signup and view all the answers

    What is the outer layer of bone tissue called?

    <p>Periosteum</p> Signup and view all the answers

    What is responsible for the large size of osteoclasts?

    <p>The fusion of many bone marrow derived cells</p> Signup and view all the answers

    Which statement accurately describes osteoblasts?

    <p>They synthesize and secrete the organic components of bone</p> Signup and view all the answers

    What is the primary role of fibroblasts in connective tissue?

    <p>Producing and maintaining extracellular components</p> Signup and view all the answers

    Which type of epithelial tissue has a high mitotic index?

    <p>Epithelial tissue</p> Signup and view all the answers

    What characterizes heterochromatin?

    <p>Clumped, dense DNA, biologically inactive</p> Signup and view all the answers

    What is the major reason for the poor regenerative capacity of cartilage?

    <p>Avascularity of the tissue</p> Signup and view all the answers

    What defines endocrine glands?

    <p>They lack ducts and lose connection to the original epithelium</p> Signup and view all the answers

    What type of secretion involves the entire cell disintegrating to release its contents?

    <p>Holocrine secretion</p> Signup and view all the answers

    What is the primary function of microvilli on epithelial cells?

    <p>Increasing surface area for absorption</p> Signup and view all the answers

    What is the purpose of a fixative in tissue preparation?

    <p>To prevent cellular breakdown by preserving cellular integrity</p> Signup and view all the answers

    What distinguishes parenchyma from stroma in an organ?

    <p>Parenchyma comprises the functional tissue, while stroma provides support</p> Signup and view all the answers

    How are tissues typically preserved in microscopic preparations?

    <p>By fixing and embedding in a solid medium like paraffin</p> Signup and view all the answers

    Study Notes

    Eukaryotic Animal Cells

    • Eukaryotic cells have distinct membrane-bound organelles.
    • Nucleus, lysosomes, and other organelles are membrane-bound.
    • Nuclear chromatin and nucleolus are non-membrane bound.
    • Heterochromatin is dense, inactive DNA.
    • Endoplasmic reticulum (ER) is rough or smooth.
    • Mitochondria are the energy source.
    • pH dictates whether a molecule is protonated or unprotonated.

    Tissue Preparation and Sectioning

    • Tissues are too thick for light microscopy.
    • Tissue preparation involves fixation to prevent digestion.
    • Fixative must diffuse through the tissue to preserve cells.
    • Macrophages are specialized in protein turnover.
    • Embedding in a solid medium allows sectioning.
    • Paraffin sections are typically 1-10 µm thick.

    Cellular Components and Tissue Types

    • Fibroblasts synthesize collagen, elastin, proteins.
    • Cationic components bind to acidic dyes.
    • In vitro studies involve cells/tissues outside the body.
    • Most organs are parenchyma and stroma.
    • Epithelial cells have a high mitotic rate.
    • Epithelia rest on connective tissue for nutrients/O2.
    • Microvilli increase surface area.
    • Secretory cells produce, store, and secrete molecules.
    • Glands are unicellular or multicellular, exocrine or endocrine.
    • Endocrine glands lack ducts.
    • Multicellular glands use merocrine, holocrine, or apocrine secretion.
    • Epithelial cells are renewed by mitosis and stem cells.
    • Connective tissues have cells, fibers, and ground substance.
    • Fibroblasts, macrophages, mast cells are key connective tissue cells.
    • Collagen, reticular, and elastic fibers are components.
    • Ground substance fills space between cells and fibers.
    • Cartilage is avascular and has a firm consistency.
    • Chondroblasts develop into chondrocytes.
    • Cartilage has three types.
    • Articular cartilage lacks perichondrium.
    • Cartilage regeneration is poor due to avascularity.

    Blood Components and Function

    • Blood is a specialized connective tissue with cells in extracellular material.
    • Plasma proteins react to form clots.
    • Albumin maintains osmotic pressure.
    • Erythrocytes form a significant volume of blood.
    • Fibrinogen converts to fibrin during clotting.
    • Red blood cells (RBCs) function without leaving the vasculature.
    • RBC shape facilitates gas exchange.
    • ABO blood groups are based on RBC antigens.
    • Leukocytes (white blood cells) are involved in defense and repair.
    • Granulocytes and agranulocytes are leukocyte types.
    • Leukocytes are phagocytic or motile outside blood vessels.
    • Neutrophils are active phagocytes.
    • Monocytes develop into macrophages.
    • Platelets promote blood clotting.
    • Hemopoiesis occurs in the liver early in development, then mostly in bone marrow.
    • Stem cells are pluripotent.
    • Myeloid and lymphoid cells form from stem cells.
    • Red bone marrow is actively involved in blood cell production.
    • Yellow bone marrow has fat cells.

    Bone Structure and Components

    • Periosteum covers the external surface of bones.
    • Osteoblasts synthesize the organic component of bone.
    • Mature osteoblasts are on bone surfaces.
    • Bone matrix has collagen fibers and other substances.
    • Osteoblasts flatten and osteocytes maintain matrix mineral structure.
    • Osteocytes communicate via gap junctions in canaliculi.
    • Osteoclasts are large cells that resorb bone.
    • Resorption cavities are the sites of bone breakdown.
    • The periosteum is a dense connective tissue with blood vessels.
    • Periosteum nourishes osseous tissue and supports bone growth/repair.

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    Description

    Explore the fascinating world of eukaryotic animal cells, focusing on the structure and function of organelles like the nucleus and mitochondria. This quiz also delves into the methods of tissue preparation and sectioning, crucial for microscopic examination. Test your knowledge on cellular components, tissue types, and the techniques used in histology.

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