Enzymes: Nature's catalysts

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Questions and Answers

What type of molecule are enzymes?

  • Lipid molecules
  • Protein molecules (correct)
  • Carbohydrate molecules
  • Nucleic acid molecules

Enzymes increase the amount of energy required for a chemical reaction to begin.

False (B)

What is the term for the energy required to start a chemical reaction?

activation energy

Enzymes speed up chemical reactions in living organisms by ______ the activation energy.

<p>lowering</p> Signup and view all the answers

Match the following enzymes with their primary function.

<p>Amylase = Breaks down starch into simpler sugars Catalase = Breaks down hydrogen peroxide into water and oxygen Pepsin = Breaks down proteins into short polypeptides Rennin = Breaks down soluble casein into insoluble casein</p> Signup and view all the answers

If an enzyme catalyzes a reaction, what effect does that enzyme have on the reaction?

<p>It speeds up the reaction. (D)</p> Signup and view all the answers

Enzymes are consumed during the reactions they catalyze.

<p>False (B)</p> Signup and view all the answers

What name suffix is commonly used for enzymes?

<p>-ase</p> Signup and view all the answers

The enzyme in saliva that breaks down starch is called ______.

<p>amylase</p> Signup and view all the answers

What is the specific region of an enzyme that binds to a substrate called?

<p>The active site (B)</p> Signup and view all the answers

An enzyme's active site is rigid and does not change shape when a substrate binds.

<p>False (B)</p> Signup and view all the answers

What is the name of the reactant that an enzyme binds to?

<p>substrate</p> Signup and view all the answers

The model of enzyme function where the active site adjusts to fit the substrate is called the ______ model.

<p>induced fit</p> Signup and view all the answers

Which of the following factors can affect enzyme activity?

<p>All of the above (D)</p> Signup and view all the answers

Increasing the temperature always increases the rate of an enzyme-catalyzed reaction.

<p>False (B)</p> Signup and view all the answers

What term describes the loss of an enzyme's structure and function due to extreme conditions?

<p>denaturation</p> Signup and view all the answers

Each enzyme has an ______ temperature at which it operates most effectively.

<p>optimal</p> Signup and view all the answers

What happens to an enzyme when the temperature goes far beyond its optimal level?

<p>It begins to denature. (B)</p> Signup and view all the answers

All enzymes function optimally at the same pH.

<p>False (B)</p> Signup and view all the answers

What is the optimal pH for the digestive enzyme pepsin?

<p>2</p> Signup and view all the answers

Enzymes have an ______ pH at which they work best.

<p>optimal</p> Signup and view all the answers

Match the following:

<p>Enzymes = Catalysts Activation energy = Energy required to start a reaction Active site = Where a substrate binds Induced fit = Enzyme changes shape when a substrate binds</p> Signup and view all the answers

According to the induced fit model, what is the direct result of a substrate binding to an enzymes active site.

<p>The enzyme's active site changes shape. (C)</p> Signup and view all the answers

Enzymes are made of carbohydrates.

<p>False (B)</p> Signup and view all the answers

What environmental factors are most likely to cause an enzyme to denature?

<p>temperature and pH</p> Signup and view all the answers

Flashcards

What are enzymes?

Enzymes are protein molecules that speed up chemical reactions in living organisms.

What is activation energy?

Energy required to start a chemical reaction.

How do enzymes speed up reactions?

Enzymes lower the activation energy of a reaction, allowing it to proceed faster.

What does it mean to catalyze?

An enzyme speeds up a reaction.

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What are catalysts?

Substances that speed up chemical reactions without being consumed in the process.

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Enzyme naming convention

Enzyme names typically end in '-ase'.

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What is amylase?

Amylase breaks down starch into simpler sugars in saliva, aiding digestion.

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What is the active site?

The portion of an enzyme that binds to the substrate (reactant).

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What are substrates?

The reactants in an enzyme-catalyzed reaction.

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What is substrate specificity?

Enzymes are specific to the chemical reaction they catalyze.

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What is the induced fit model?

This model states the active site changes shape to better fit the substrate.

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Factors affecting enzyme activity

Temperature and pH can affect enzyme activity.

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Temperature's effect on enzyme activity

As temperature increases, enzyme-catalyzed reactions initially speed up.

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What happens when enzymes denature?

Above a certain temperature, the enzyme begins to denature and becomes less effective.

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Optimal temperature

The temperature at which an enzyme works best.

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Optimal pH

Enzymes also have an optimal pH at which they work best.

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What is Pepsin?

The digestive enzyme that works best in the acidic environment of the stomach (pH 2).

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Study Notes

  • Enzymes for everyone

Enzymes Speed Up Reactions

  • Enzymes are protein molecules that accelerate chemical reactions in living organisms
  • Enzymes are essential for life because many bodily reactions would be too slow to sustain life without them

Activation Energy

  • All chemical reactions need energy to start
  • Activation energy is the energy required to start a chemical reaction
  • Enzymes work by lowering the activation energy of a reaction
  • Reactions occur more quickly since less energy is needed to start them

Catalysts

  • An enzyme catalyzes a reaction when it speeds it up
  • Enzymes are also called catalysts

Enzyme Names

  • Enzyme names typically end in "-ase"
  • Amylase is an enzyme present in saliva
  • Amylase present in saliva breaks down starch into simpler sugars which simplifies digestion

Enzyme Structure

  • An enzyme's active site is the part that binds to the substrate (reactant)
  • Substrates are the reactants in an enzyme-catalyzed reaction
  • Enzymes are specific to the chemical reactions they catalyze which relates to "substrate specificity"

How Enzymes Work

  • Enzymes have a complex three-dimensional surface to which particular reactants (called substrates) fit, like a hand in a glove
  • Enzymes and their substrate(s) will bind tightly together, forming an enzyme-substrate complex
  • Binding brings key atoms close and stresses key covalent bonds
  • A chemical reaction occurs within the active site, which forms the product
  • The product separates, freeing the enzyme for further work

Models of Enzyme Function

  • Lock and Key Model of enzyme function
  • Induced Fit Model of enzyme function
  • The induced fit model for how catalysis is more widely accepted
  • A conformational or structural change places stress on bonds, causing them to break, which is necessary for creating products

Examples

  • Catalase: Converts hydrogen peroxide (H2O2) into oxygen gas and water
  • Diastase: Converts starch into maltose
  • Pectinase: Converts pectin (in plant cell walls) into simple sugars and releases juices from cells
  • Pepsin: Converts protein into short polypeptides
  • Rennin: Converts soluble casein (milk protein) into insoluble casein (curdled milk)

Factors Impacting Enzyme Activity

  • Temperature and pH influence an enzyme's activity
  • Enzyme-catalyzed reactions accelerate with an increase in temperature
  • Enzymes begin to denature if the temperature exceeds a specific point, which makes them less effective
  • Every enzyme has an optimal temperature at which it works best
  • Typical human enzymes work best at about 37°C
  • Heat-tolerant organism enzyme works best at approximately 76°C
  • Enzymes have an optimal pH at which they work best
  • Digestive enzyme, pepsin, works best in the acidic environment of the stomach, pH 2

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