20 Questions
What does the 'I' in 'IMViC' stand for?
Indole
What is the medium used for the Indole test?
Tryptone broth
How is a positive result indicated in the Indole test?
Presence of a red or red-violet color
Name one organism that shows a positive Indole test result.
E.coli
What does the 'M' in 'IMViC' stand for?
Methyl red
What are the two separate tests that use one broth as part of the IMViC tests?
Methyl Red and Voges–Proskauer
What is the purpose of the Methyl Red test?
To detect acidic end products of glucose metabolism
Which bacteria exhibit a positive Methyl Red test?
Klebsiella pneumoniae and Enterobacter aerogenes
What is the principle of the Voges–Proskauer (VP) test?
To detect the neutral end products of glucose metabolism
Which bacteria exhibit a positive Voges–Proskauer (VP) test?
Klebsiella, Enterobacter aerogenes, and Citrate utilization Test
What does the Citrate utilization test distinguish between?
It distinguishes between members of the Enterobacteriaceae family based on their ability to use citrate as a sole carbon source.
What is the color change observed in a positive Citrate utilization test?
The color changes from green to blue
Which bacteria exhibit a positive Citrate utilization test?
Klebsiella pneumoniae and Proteus mirabilis
What is the purpose of the Urease test?
To test the ability of an organism to produce urease, which hydrolyzes urea to ammonia and carbon dioxide
What color change is observed in a positive Urease test?
The color changes from orange yellow to bright pink
Which bacteria exhibit a positive Urease test?
Proteus, Klebsiella, and Yersinia species
What is the purpose of the Oxidase test?
To determine if an organism possesses the cytochrome oxidase enzyme
Which family of bacteria are typically Oxidase negative?
Enterobacteriaceae
What is the purpose of the Catalase test?
To determine if the organism is catalase positive
Which family of bacteria are catalase positive?
Members of the Enterobacteriaceae family
Study Notes
IMViC Tests
- 'I' in IMViC stands for Indole test
- Medium used for Indole test is peptone broth
- Positive result indicated in Indole test by formation of a red ring at the surface of the broth
- One organism that shows a positive Indole test result is Escherichia coli
Methyl Red Test
- 'M' in IMViC stands for Methyl Red test
- Two separate tests that use one broth as part of IMViC tests are Methyl Red and Voges-Proskauer (VP) tests
- Purpose of Methyl Red test is to detect acid production from glucose fermentation
- Bacteria that exhibit a positive Methyl Red test are Escherichia coli and Enterobacter
Voges-Proskauer (VP) Test
- Principle of VP test is to detect acetoin production from glucose fermentation
- Bacteria that exhibit a positive VP test are Klebsiella and Enterobacter
Citrate Utilization Test
- Citrate utilization test distinguishes between bacteria that can utilize citrate as a sole source of carbon and energy
- Color change observed in a positive Citrate test is a change from green to blue
- Bacteria that exhibit a positive Citrate utilization test are Klebsiella and Enterobacter
Urease Test
- Purpose of Urease test is to detect urease enzyme production
- Color change observed in a positive Urease test is a change from yellow to pink
- Bacteria that exhibit a positive Urease test are Proteus and Klebsiella
Oxidase and Catalase Tests
- Purpose of Oxidase test is to detect cytochrome c oxidase production
- Family of bacteria that are typically Oxidase negative are Enterobacteriaceae
- Purpose of Catalase test is to detect catalase enzyme production
- Family of bacteria that are catalase positive are Staphylococcus and Bacillus
Test your knowledge on the IMViC tests used for identifying members of the Enterobacteriaceae family. Each letter in 'IMViC' represents a specific test, including the Indole, Methyl red, Voges-Proskauer, and Citrate tests. This quiz covers the Indole test, its medium, reagent, and its purpose in bacterial identification.
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