Enterobacteriaceae Identification: IMViC Tests Quiz
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Questions and Answers

What does the 'I' in 'IMViC' stand for?

Indole

What is the medium used for the Indole test?

Tryptone broth

How is a positive result indicated in the Indole test?

Presence of a red or red-violet color

Name one organism that shows a positive Indole test result.

<p>E.coli</p> Signup and view all the answers

What does the 'M' in 'IMViC' stand for?

<p>Methyl red</p> Signup and view all the answers

What are the two separate tests that use one broth as part of the IMViC tests?

<p>Methyl Red and Voges–Proskauer</p> Signup and view all the answers

What is the purpose of the Methyl Red test?

<p>To detect acidic end products of glucose metabolism</p> Signup and view all the answers

Which bacteria exhibit a positive Methyl Red test?

<p>Klebsiella pneumoniae and Enterobacter aerogenes</p> Signup and view all the answers

What is the principle of the Voges–Proskauer (VP) test?

<p>To detect the neutral end products of glucose metabolism</p> Signup and view all the answers

Which bacteria exhibit a positive Voges–Proskauer (VP) test?

<p>Klebsiella, Enterobacter aerogenes, and Citrate utilization Test</p> Signup and view all the answers

What does the Citrate utilization test distinguish between?

<p>It distinguishes between members of the Enterobacteriaceae family based on their ability to use citrate as a sole carbon source.</p> Signup and view all the answers

What is the color change observed in a positive Citrate utilization test?

<p>The color changes from green to blue</p> Signup and view all the answers

Which bacteria exhibit a positive Citrate utilization test?

<p>Klebsiella pneumoniae and Proteus mirabilis</p> Signup and view all the answers

What is the purpose of the Urease test?

<p>To test the ability of an organism to produce urease, which hydrolyzes urea to ammonia and carbon dioxide</p> Signup and view all the answers

What color change is observed in a positive Urease test?

<p>The color changes from orange yellow to bright pink</p> Signup and view all the answers

Which bacteria exhibit a positive Urease test?

<p>Proteus, Klebsiella, and Yersinia species</p> Signup and view all the answers

What is the purpose of the Oxidase test?

<p>To determine if an organism possesses the cytochrome oxidase enzyme</p> Signup and view all the answers

Which family of bacteria are typically Oxidase negative?

<p>Enterobacteriaceae</p> Signup and view all the answers

What is the purpose of the Catalase test?

<p>To determine if the organism is catalase positive</p> Signup and view all the answers

Which family of bacteria are catalase positive?

<p>Members of the Enterobacteriaceae family</p> Signup and view all the answers

Flashcards

Indole Test

Detects the presence of indole, a byproduct of tryptophan metabolism.

Medium for Indole Test

Tryptone broth is used as the medium for the Indole test.

Positive Indole Test

A positive Indole test is indicated by a red or red-violet color.

Bacterium Positive for Indole

E. coli is a bacterium that produces indole during metabolism.

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Methyl Red Test

The Methyl Red test detects acidic end products from glucose metabolism.

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Shared Broth

The Methyl Red test and the Voges-Proskauer test both use the same broth.

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Purpose of Methyl Red Test

The Methyl Red test aims to detect the presence of acidic end products from glucose metabolism.

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Bacteria Positive for Methyl Red

Klebsiella pneumoniae and Enterobacter aerogenes are bacteria that produce acidic end products in glucose metabolism.

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Voges-Proskauer (VP) Test

The Voges-Proskauer (VP) test detects neutral end products of glucose metabolism.

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Bacteria Positive for VP

Klebsiella, Enterobacter aerogenes, and Citrate utilization positive bacteria show positive results in the Voges-Proskauer (VP) test.

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Citrate Utilization Test

The Citrate utilization test differentiates bacteria by their ability to use citrate as their sole carbon source.

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Positive Citrate Test

A positive Citrate utilization test results in a color change from green to blue.

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Bacteria Positive for Citrate

Klebsiella pneumoniae and Proteus mirabilis are bacteria that can utilize citrate as their sole carbon source.

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Urease Test

The Urease test examines the ability of an organism to produce urease, an enzyme that breaks down urea.

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Positive Urease Test

A bright pink color change from an orange yellow indicates a positive Urease test.

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Bacteria Positive for Urease

Proteus, Klebsiella, and Yersinia species are known to produce urease.

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Oxidase Test

The Oxidase test detects the presence of cytochrome oxidase, a key enzyme in the electron transport chain.

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Oxidase Negative Bacteria

The Enterobacteriaceae family is generally known for being negative in the Oxidase test.

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Catalase Test

The Catalase test determines the presence of the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen.

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Catalase Positive Bacteria

The Enterobacteriaceae family is generally considered catalase positive.

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Study Notes

IMViC Tests

  • 'I' in IMViC stands for Indole test
  • Medium used for Indole test is peptone broth
  • Positive result indicated in Indole test by formation of a red ring at the surface of the broth
  • One organism that shows a positive Indole test result is Escherichia coli

Methyl Red Test

  • 'M' in IMViC stands for Methyl Red test
  • Two separate tests that use one broth as part of IMViC tests are Methyl Red and Voges-Proskauer (VP) tests
  • Purpose of Methyl Red test is to detect acid production from glucose fermentation
  • Bacteria that exhibit a positive Methyl Red test are Escherichia coli and Enterobacter

Voges-Proskauer (VP) Test

  • Principle of VP test is to detect acetoin production from glucose fermentation
  • Bacteria that exhibit a positive VP test are Klebsiella and Enterobacter

Citrate Utilization Test

  • Citrate utilization test distinguishes between bacteria that can utilize citrate as a sole source of carbon and energy
  • Color change observed in a positive Citrate test is a change from green to blue
  • Bacteria that exhibit a positive Citrate utilization test are Klebsiella and Enterobacter

Urease Test

  • Purpose of Urease test is to detect urease enzyme production
  • Color change observed in a positive Urease test is a change from yellow to pink
  • Bacteria that exhibit a positive Urease test are Proteus and Klebsiella

Oxidase and Catalase Tests

  • Purpose of Oxidase test is to detect cytochrome c oxidase production
  • Family of bacteria that are typically Oxidase negative are Enterobacteriaceae
  • Purpose of Catalase test is to detect catalase enzyme production
  • Family of bacteria that are catalase positive are Staphylococcus and Bacillus

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Description

Test your knowledge on the IMViC tests used for identifying members of the Enterobacteriaceae family. Each letter in 'IMViC' represents a specific test, including the Indole, Methyl red, Voges-Proskauer, and Citrate tests. This quiz covers the Indole test, its medium, reagent, and its purpose in bacterial identification.

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