Enterobacteriaceae IMViC Tests Quiz: Master Identification

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Questions and Answers

What does the 'I' in 'IMViC' stand for?

Indole

What is the medium used for the Indole test?

Tryptone broth

How is a positive result indicated in the Indole test?

Presence of a red or red-violet color

Name one organism that shows a positive Indole test result.

E.coli Signup and view all the answers

What does the 'M' in 'IMViC' stand for?

Methyl red Signup and view all the answers

What are the two separate tests that use one broth as part of the IMViC tests?

Methyl Red and Voges–Proskauer Signup and view all the answers

What is the purpose of the Methyl Red test?

To detect acidic end products of glucose metabolism Signup and view all the answers

Which bacteria exhibit a positive Methyl Red test?

Klebsiella pneumoniae and Enterobacter aerogenes Signup and view all the answers

What is the principle of the Voges–Proskauer (VP) test?

To detect the neutral end products of glucose metabolism Signup and view all the answers

Which bacteria exhibit a positive Voges–Proskauer (VP) test?

Klebsiella, Enterobacter aerogenes, and Citrate utilization Test Signup and view all the answers

What does the Citrate utilization test distinguish between?

It distinguishes between members of the Enterobacteriaceae family based on their ability to use citrate as a sole carbon source. Signup and view all the answers

What is the color change observed in a positive Citrate utilization test?

The color changes from green to blue Signup and view all the answers

Which bacteria exhibit a positive Citrate utilization test?

Klebsiella pneumoniae and Proteus mirabilis Signup and view all the answers

What is the purpose of the Urease test?

To test the ability of an organism to produce urease, which hydrolyzes urea to ammonia and carbon dioxide Signup and view all the answers

What color change is observed in a positive Urease test?

The color changes from orange yellow to bright pink Signup and view all the answers

Which bacteria exhibit a positive Urease test?

Proteus, Klebsiella, and Yersinia species Signup and view all the answers

What is the purpose of the Oxidase test?

To determine if an organism possesses the cytochrome oxidase enzyme Signup and view all the answers

Which family of bacteria are typically Oxidase negative?

Enterobacteriaceae Signup and view all the answers

What is the purpose of the Catalase test?

To determine if the organism is catalase positive Signup and view all the answers

Which family of bacteria are catalase positive?

Members of the Enterobacteriaceae family Signup and view all the answers

Study Notes

IMViC Tests

'I' in IMViC stands for Indole test
Medium used for Indole test is peptone broth
Positive result indicated in Indole test by formation of a red ring at the surface of the broth
One organism that shows a positive Indole test result is Escherichia coli

Methyl Red Test

'M' in IMViC stands for Methyl Red test
Two separate tests that use one broth as part of IMViC tests are Methyl Red and Voges-Proskauer (VP) tests
Purpose of Methyl Red test is to detect acid production from glucose fermentation
Bacteria that exhibit a positive Methyl Red test are Escherichia coli and Enterobacter

Voges-Proskauer (VP) Test

Principle of VP test is to detect acetoin production from glucose fermentation
Bacteria that exhibit a positive VP test are Klebsiella and Enterobacter

Citrate Utilization Test

Citrate utilization test distinguishes between bacteria that can utilize citrate as a sole source of carbon and energy
Color change observed in a positive Citrate test is a change from green to blue
Bacteria that exhibit a positive Citrate utilization test are Klebsiella and Enterobacter

Urease Test

Purpose of Urease test is to detect urease enzyme production
Color change observed in a positive Urease test is a change from yellow to pink
Bacteria that exhibit a positive Urease test are Proteus and Klebsiella

Oxidase and Catalase Tests

Purpose of Oxidase test is to detect cytochrome c oxidase production
Family of bacteria that are typically Oxidase negative are Enterobacteriaceae
Purpose of Catalase test is to detect catalase enzyme production
Family of bacteria that are catalase positive are Staphylococcus and Bacillus

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Description

Test your knowledge on the IMViC tests used for identifying members of the Enterobacteriaceae family. Each letter in 'IMViC' represents a specific test, including the Indole, Methyl red, Voges-Proskauer, and Citrate tests. This quiz covers the Indole test, its medium, reagent, and its purpose in bacterial identification.