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Enterobacteriaceae Identification: IMViC Tests
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Enterobacteriaceae Identification: IMViC Tests

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Questions and Answers

What does the letter 'I' in 'IMViC' stand for?

Indole

What reagent is used to detect indole production in the Indole test?

Kovac’s or Ehrlich’s reagent

What is the appearance of a negative result in the Indole test?

Yellow

Which organisms show a positive result in the Indole test?

<p>E.coli, P.vulgaris, Shigella spp., Klebsiella oxytoca</p> Signup and view all the answers

What are the two separate tests that use the Methyl Red – Voges Proskauer broth?

<p>Methyl Red (MR) and Voges–Proskauer (VP) Test</p> Signup and view all the answers

What is the purpose of the Methyl Red (MR) test?

<p>To detect the acidic end product of glucose metabolism</p> Signup and view all the answers

What is the purpose of the Methyl Red (MR) test?

<p>To detect mixed acid fermentation</p> Signup and view all the answers

Which bacteria produce a positive Methyl Red (MR) test?

<p>Enterobacter aerogenes and Klebsiella pneumoniae</p> Signup and view all the answers

What is the purpose of the Voges–Proskauer (VP) test?

<p>To detect the neutral end products of glucose metabolism</p> Signup and view all the answers

Which bacteria produce a positive Voges–Proskauer (VP) test?

<p>Klebsiella, Enterobacter aerogenes, and Citrate-positive bacteria</p> Signup and view all the answers

What is the purpose of the Citrate utilization test?

<p>To distinguish between members of the Enterobacteriaceae family based on citrate utilization</p> Signup and view all the answers

What is the indicator used in the Urease test?

<p>Phenol red</p> Signup and view all the answers

Which bacteria produce a positive Urease test?

<p>Proteus, Klebsiella, and Yersinia species</p> Signup and view all the answers

What does the oxidase test determine?

<p>If an organism possesses the cytochrome oxidase enzyme</p> Signup and view all the answers

Which organisms are typically Oxidase negative?

<p>Enterobacteriaceae</p> Signup and view all the answers

What does the Catalase test determine for members of the Enterobacteriaceae family?

<p>Catalase positive</p> Signup and view all the answers

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