Microbiology IMViC Tests Analysis

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Questions and Answers

Which bacterial species is identified as decarboxylating Lysine?

  • Enterobacter cloacae
  • Enterobacter agglomerans
  • Serratia marcescens (correct)
  • Klebsiella pneumoniae (correct)

What does a positive result in the PR Sucrose test indicate about a bacteria?

  • The bacteria is motile.
  • The bacteria cannot decarboxylate Ornithine.
  • The bacteria is Gram-negative.
  • The bacteria ferments sucrose. (correct)

Which test indicates if an organism ferments glucose and produces hydrogen?

  • Lysine Decarboxylase
  • PR Sucrose
  • Motility
  • PR Glucose w/ Gas (correct)

Which species shows motility based on the results?

<p>Klebsiella pneumoniae (C), Enterobacter cloacae (D)</p> Signup and view all the answers

What does a box filled with a positive sign (+) signify in the results table?

<p>The organism decarboxylates the tested amino acid. (D)</p> Signup and view all the answers

Which of the following bacteria is Gram-positive?

<p>Klebsiella pneumoniae (B)</p> Signup and view all the answers

What does the variable result (+/-) represent about an organism?

<p>The result is ambiguous and may vary. (B)</p> Signup and view all the answers

Which bacterium has a positive result in both the Ornithine Decarboxylase and Motility tests?

<p>Serratia marcescens (A), Klebsiella pneumoniae (B)</p> Signup and view all the answers

Flashcards

Bacterial Identification Tests

A series of tests used to determine bacterial species based on their biochemical properties.

Lysine Decarboxylase Test

A test to determine if a bacterium converts Lysine into a different molecule.

PR Sucrose Test

Checks if a bacterium can ferment sucrose and produce acid.

Ornithine Decarboxylase Test

A test that identifies organisms that convert Ornithine into another chemical.

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Motility Test

Determines if a bacterium can move independently.

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PR Glucose w/ Gas Test

Tests if a bacterium ferments glucose and produces gas as a byproduct.

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Gram-positive/Gram-negative

Two categories of bacteria based on the cell wall structure revealed during staining.

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Biochemical Characteristics

The specific chemical reactions and processes that an organism can perform.

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Study Notes

IMViC Results

  • IMViC tests are used to identify bacteria based on their metabolic reactions.

Figure 1

  • Lysine Decarboxylase:

    • Enterobacter agglomerans group: Negative
    • Enterobacter cloacae: Positive
    • Enterobacter taylorae: Negative
    • Klebsiella pneumoniae: Positive
    • Serratia marcescens: Positive
  • PR Sucrose:

    • Enterobacter taylorae: Positive
  • Motility:

    • Enterobacter agglomerans group: Negative
    • Enterobacter cloacae: Negative
    • Enterobacter taylorae: Negative
    • Klebsiella pneumoniae: Positive
    • Serratia marcescens: Positive
  • Ornithine Decarboxylase:

    • Enterobacter agglomerans group: Positive
    • Enterobacter cloacae: Positive
    • Enterobacter taylorae: Negative
  • PR Glucose w/ Gas:

    • Enterobacter aerogenes: Positive
    • Klebsiella pneumoniae: Negative
    • Serratia marcescens: Negative

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