ELISA Test Principles and Concepts

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What are the key components involved in the ELISA test?

Antigen and Antibody

What is the difference between Direct and Indirect ELISA?

Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while Indirect ELISA utilizes a primary unlabeled antibody in conjunction with a labeled secondary antibody.

What are the applications of Indirect ELISA?

Determining small molecules like T₃, T₄ & Progesterone

What is the main purpose of measuring antibody levels using ELISA?

To measure vaccine antibody levels

What are the disadvantages of ELISA?

Measurement of enzyme activity can be complex, affected by plasma constituents, expensive kits, specific to particular antigen, and prone to false positives/negatives.

This quiz covers the principles and concepts of the ELISA test, including the introduction, the principle of the test, basic immunology response, and key concepts like antigens and antibodies. It also includes information on enzyme conjugate substrates, non-competitive ELISA, direct and indirect ELISA.

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