ELISA Test Principles

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Questions and Answers

What are the applications of ELISA?

Measuring antibody levels (vaccines), detecting hormonal changes (pregnancy), and detecting circulatory inflammatory markers (cytokines).

What is the difference between Direct and Indirect ELISA?

Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while Indirect ELISA uses a primary un-labeled antibody in conjunction with a labeled secondary antibody.

What are the key components of the ELISA test?

Antigen and antibody

What are the disadvantages of ELISA?

<p>Measurement of enzyme activity can be complex, enzyme activity may be affected by plasma constituents, kits are expensive, very specific to particular antigens, and false positive/negative results are possible.</p> Signup and view all the answers

Flashcards

Applications of ELISA?

Measuring antibody levels (vaccines), detecting hormonal changes (pregnancy), and detecting circulatory inflammatory markers (cytokines).

Direct vs. Indirect ELISA?

Direct ELISA uses a primary labeled antibody that reacts directly with the antigen. Indirect ELISA uses a primary un-labeled antibody with a labeled secondary antibody.