ELISA Test Principles

Measuring antibody levels (vaccines), detecting hormonal changes (pregnancy), and detecting circulatory inflammatory markers (cytokines).

Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while Indirect ELISA uses a primary un-labeled antibody in conjunction with a labeled secondary antibody.

Antigen and antibody

<p>Measurement of enzyme activity can be complex, enzyme activity may be affected by plasma constituents, kits are expensive, very specific to particular antigens, and false positive/negative results are possible.</p> Signup and view all the answers