15 Questions
What is the primary function of an ELISA reader?
To read the fluorescent or chromogenic response of the ELISA in a 96-well plate
Which instruments are combined in an ELISA reader?
Fluorimeter and spectrophotometer
What principle is the ELISA reader based on?
Beer-Lambert law
What types of substances can be detected and quantified using the ELISA technique?
Peptides, proteins, antibodies, and hormones
What does 'A' represent in the Beer-Lambert law for the ELISA reader?
Absorbance
What is the primary purpose of an ELISA reader?
To read the fluorescent or chromogenic response of the ELISA in a 96-well plate
Which instruments are combined in an ELISA reader?
Fluorimeter and spectrophotometer
What does the Beer-Lambert law state about absorbance in the ELISA reader?
Absorbance has a linear relationship with the length of light and the concentration of a sample
What is T in the Beer-Lambert law for the ELISA reader?
Transmittance; the fraction of light passing through the sample
Which substances can be detected and quantified using the ELISA technique?
Peptides, proteins, antibodies, and hormones
An ELISA reader is only able to read the fluorescent response of the ELISA in a 96-well plate.
False
The Beer-Lambert law states that the absorbance of the solution has a linear relationship with the concentration of the sample.
True
The fraction of light passing through the sample is expressed as T= rac{It}{Io}.
True
The ELISA technique is designed for detecting and quantifying substances such as nucleic acids and carbohydrates.
False
An ELISA reader is both a fluorimeter and a mass spectrometer.
False
Study Notes
ELISA Reader Function and Principle
- The primary function of an ELISA reader is to detect and quantify substances such as nucleic acids and carbohydrates.
- An ELISA reader is based on the principle of absorbance, which is measured using the Beer-Lambert law.
Instruments Combined in an ELISA Reader
- An ELISA reader combines a fluorimeter and a spectrophotometer (not a mass spectrometer).
Beer-Lambert Law and Absorbance
- The Beer-Lambert law states that the absorbance of the solution has a linear relationship with the concentration of the sample.
- The law is expressed as A = εcl, where A is the absorbance, ε is the molar absorptivity, c is the concentration of the sample, and l is the path length.
- The fraction of light passing through the sample is expressed as T = It/Io, where T is the transmittance, It is the intensity of the transmitted light, and Io is the initial intensity of the light.
ELISA Technique and Detection
- The ELISA technique is designed for detecting and quantifying substances such as nucleic acids and carbohydrates.
- The ELISA reader can detect and quantify these substances in a 96-well plate.
Note
- An ELISA reader does not read the fluorescent response of the ELISA, as it is a spectrophotometer that measures absorbance, not fluorescence.
Test your knowledge about ELISA readers and their use in reading the fluorescent or chromogenic response of ELISA in a 96-well plate. Learn about the design and quantitative information provided by ELISA readers.
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