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Questions and Answers
What is the purpose of ELISA?
What is the purpose of ELISA?
- To measure the concentration of enzymes in a sample
- To detect the presence of a specific protein in a liquid sample (correct)
- To determine the pH level of a liquid sample
- To identify the type of antibodies present in a sample
What is the most common detectable signal produced in ELISA?
What is the most common detectable signal produced in ELISA?
- Color change (correct)
- Change in pH
- Release of gas bubbles
- Formation of a precipitate
In ELISA, what is the role of the enzyme-linked antibody?
In ELISA, what is the role of the enzyme-linked antibody?
- It binds to the antigen and produces a detectable signal (correct)
- It changes the pH of the liquid sample
- It measures the concentration of the protein in the sample
- It removes unbound antigens from the sample
In the simplest form of ELISA, where are antigens from the sample to be tested attached?
In the simplest form of ELISA, where are antigens from the sample to be tested attached?
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What was the year when the enzyme-linked immunosorbent assay (ELISA) was first described?
What was the year when the enzyme-linked immunosorbent assay (ELISA) was first described?
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Study Notes
ELISA Overview
- The purpose of ELISA (Enzyme-Linked Immunosorbent Assay) is to detect and quantify substances such as proteins, peptides, antibodies, and hormones.
ELISA Mechanism
- The most common detectable signal produced in ELISA is a color change.
- The enzyme-linked antibody plays a role in catalyzing a chemical reaction to produce the detectable signal.
Antigen Attachment
- In the simplest form of ELISA, antigens from the sample to be tested are attached to a microtiter plate well.
History of ELISA
- The enzyme-linked immunosorbent assay (ELISA) was first described in the year 1971.
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Description
Test your knowledge of the enzyme-linked immunosorbent assay (ELISA) with this quiz. Explore the principles, applications, and significance of this widely used biochemistry assay.
