5 Questions
What is the purpose of ELISA?
To detect the presence of a specific protein in a liquid sample
What is the most common detectable signal produced in ELISA?
Color change
In ELISA, what is the role of the enzyme-linked antibody?
It binds to the antigen and produces a detectable signal
In the simplest form of ELISA, where are antigens from the sample to be tested attached?
To a surface
What was the year when the enzyme-linked immunosorbent assay (ELISA) was first described?
1971
Study Notes
ELISA Overview
- The purpose of ELISA (Enzyme-Linked Immunosorbent Assay) is to detect and quantify substances such as proteins, peptides, antibodies, and hormones.
ELISA Mechanism
- The most common detectable signal produced in ELISA is a color change.
- The enzyme-linked antibody plays a role in catalyzing a chemical reaction to produce the detectable signal.
Antigen Attachment
- In the simplest form of ELISA, antigens from the sample to be tested are attached to a microtiter plate well.
History of ELISA
- The enzyme-linked immunosorbent assay (ELISA) was first described in the year 1971.
Test your knowledge of the enzyme-linked immunosorbent assay (ELISA) with this quiz. Explore the principles, applications, and significance of this widely used biochemistry assay.
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