ELISA Quiz
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Questions and Answers

What is the purpose of ELISA?

  • To measure the concentration of enzymes in a sample
  • To detect the presence of a specific protein in a liquid sample (correct)
  • To determine the pH level of a liquid sample
  • To identify the type of antibodies present in a sample
  • What is the most common detectable signal produced in ELISA?

  • Color change (correct)
  • Change in pH
  • Release of gas bubbles
  • Formation of a precipitate
  • In ELISA, what is the role of the enzyme-linked antibody?

  • It binds to the antigen and produces a detectable signal (correct)
  • It changes the pH of the liquid sample
  • It measures the concentration of the protein in the sample
  • It removes unbound antigens from the sample
  • In the simplest form of ELISA, where are antigens from the sample to be tested attached?

    <p>To a surface</p> Signup and view all the answers

    What was the year when the enzyme-linked immunosorbent assay (ELISA) was first described?

    <p>1971</p> Signup and view all the answers

    Study Notes

    ELISA Overview

    • The purpose of ELISA (Enzyme-Linked Immunosorbent Assay) is to detect and quantify substances such as proteins, peptides, antibodies, and hormones.

    ELISA Mechanism

    • The most common detectable signal produced in ELISA is a color change.
    • The enzyme-linked antibody plays a role in catalyzing a chemical reaction to produce the detectable signal.

    Antigen Attachment

    • In the simplest form of ELISA, antigens from the sample to be tested are attached to a microtiter plate well.

    History of ELISA

    • The enzyme-linked immunosorbent assay (ELISA) was first described in the year 1971.

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    Quiz Team

    Description

    Test your knowledge of the enzyme-linked immunosorbent assay (ELISA) with this quiz. Explore the principles, applications, and significance of this widely used biochemistry assay.

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