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ELISA

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Questions and Answers

Which of the following is true about ELISA?

ELISA is a type of PCR technique used to amplify DNA in a liquid sample.

ELISA is a type of enzyme immunoassay used to detect the presence of a ligand in a liquid sample. (correct)

ELISA is a type of mass spectrometry used to analyze the composition of a liquid sample.

ELISA is a type of chromatography used to separate proteins in a liquid sample.

What is the purpose of immobilizing the antigen in an ELISA?

To remove any non-specifically bound proteins or antibodies.

To allow the antigen to bind with the detection antibody. (correct)

To produce a color change in the subsequent reaction.

To prevent the antigen from binding with the detection antibody.

What is the role of the detection antibody in an ELISA?

To form a complex with the antigen. (correct)

To immobilize the antigen on a solid support.

To remove any non-specifically bound proteins or antibodies.

To produce a color change in the subsequent reaction.

Which of the following is true about ELISA plates?

ELISA plates have the reaction products immunosorbed on the solid phase Signup and view all the answers

What is the purpose of adding an enzymatic substrate in ELISA?

To generate a signal that can be quantified Signup and view all the answers

What is the name of the technique that uses dry strips for analysis?

Reflectometry Signup and view all the answers

What is the historical alternative to ELISA for conducting immunoassays?

Radioimmunoassay Signup and view all the answers

Which of the following is a major disadvantage of the direct ELISA method?

The antigen immobilization is not specific Signup and view all the answers

What is the purpose of linking the enzyme to an appropriate antibody in the ELISA technique?

To amplify the color change Signup and view all the answers

Which type of ELISA involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well?

Direct ELISA Signup and view all the answers

What is the purpose of using a spectrometer in the direct ELISA method?

To give quantitative values for color strength Signup and view all the answers

Study Notes

ELISA Basics

ELISA (Enzyme-Linked Immunosorbent Assay) is a technique used for immunoassays.
ELISA involves immobilizing the antigen to facilitate the reaction.

ELISA Process

The detection antibody plays a crucial role in ELISA by binding to the antigen, allowing for detection.
ELISA plates are used to facilitate the reaction and are typically 96-well microtiter plates.
An enzymatic substrate is added to ELISA to enable the detection of the antigen-antibody reaction.
The enzyme linked to the antibody catalyzes the substrate, producing a measurable signal.

Other Techniques

The dry strip analysis technique is known as Lateral Flow Immunoassay (LFIA).
Radioimmunoassay (RIA) is a historical alternative to ELISA for conducting immunoassays.

Direct ELISA

A major disadvantage of the direct ELISA method is that it can be less sensitive than other ELISA methods.
The purpose of linking the enzyme to an appropriate antibody is to enable detection of the antigen-antibody reaction.
The direct ELISA method involves using a spectrometer to measure the signal produced by the enzyme-substrate reaction.

ELISA Variations

The indirect ELISA method involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well, a process known as blocking.

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Description

Test your knowledge on ELISA - a commonly used analytical biochemistry assay that detects the presence of proteins in liquid samples using antibodies. Learn about its history, principles, and applications in this informative quiz.