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Questions and Answers
Which of the following is true about ELISA?
Which of the following is true about ELISA?
- ELISA is a type of PCR technique used to amplify DNA in a liquid sample.
- ELISA is a type of enzyme immunoassay used to detect the presence of a ligand in a liquid sample. (correct)
- ELISA is a type of mass spectrometry used to analyze the composition of a liquid sample.
- ELISA is a type of chromatography used to separate proteins in a liquid sample.
What is the purpose of immobilizing the antigen in an ELISA?
What is the purpose of immobilizing the antigen in an ELISA?
- To remove any non-specifically bound proteins or antibodies.
- To allow the antigen to bind with the detection antibody. (correct)
- To produce a color change in the subsequent reaction.
- To prevent the antigen from binding with the detection antibody.
What is the role of the detection antibody in an ELISA?
What is the role of the detection antibody in an ELISA?
- To form a complex with the antigen. (correct)
- To immobilize the antigen on a solid support.
- To remove any non-specifically bound proteins or antibodies.
- To produce a color change in the subsequent reaction.
Which of the following is true about ELISA plates?
Which of the following is true about ELISA plates?
What is the purpose of adding an enzymatic substrate in ELISA?
What is the purpose of adding an enzymatic substrate in ELISA?
What is the name of the technique that uses dry strips for analysis?
What is the name of the technique that uses dry strips for analysis?
What is the historical alternative to ELISA for conducting immunoassays?
What is the historical alternative to ELISA for conducting immunoassays?
Which of the following is a major disadvantage of the direct ELISA method?
Which of the following is a major disadvantage of the direct ELISA method?
What is the purpose of linking the enzyme to an appropriate antibody in the ELISA technique?
What is the purpose of linking the enzyme to an appropriate antibody in the ELISA technique?
Which type of ELISA involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well?
Which type of ELISA involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well?
What is the purpose of using a spectrometer in the direct ELISA method?
What is the purpose of using a spectrometer in the direct ELISA method?
Flashcards
ELISA Purpose
ELISA Purpose
ELISA is a technique to detect a substance (ligand) in a liquid sample using enzymes.
Antigen Immobilization in ELISA
Antigen Immobilization in ELISA
Immobilizing the antigen (target molecule) allows antibodies to bind to it, making detection easier.
Detection Antibody Role
Detection Antibody Role
The detection antibody binds to the target antigen, forming a complex for detection.
ELISA Plate Function
ELISA Plate Function
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Enzymatic Substrate Purpose
Enzymatic Substrate Purpose
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Reflectometry Technique
Reflectometry Technique
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Radioimmunoassay (RIA)
Radioimmunoassay (RIA)
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Direct ELISA Disadvantage
Direct ELISA Disadvantage
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Enzyme-Antibody Link Purpose
Enzyme-Antibody Link Purpose
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Blocking Solution in ELISA
Blocking Solution in ELISA
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Spectrometer in ELISA
Spectrometer in ELISA
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Study Notes
ELISA Basics
- ELISA (Enzyme-Linked Immunosorbent Assay) is a technique used for immunoassays.
- ELISA involves immobilizing the antigen to facilitate the reaction.
ELISA Process
- The detection antibody plays a crucial role in ELISA by binding to the antigen, allowing for detection.
- ELISA plates are used to facilitate the reaction and are typically 96-well microtiter plates.
- An enzymatic substrate is added to ELISA to enable the detection of the antigen-antibody reaction.
- The enzyme linked to the antibody catalyzes the substrate, producing a measurable signal.
Other Techniques
- The dry strip analysis technique is known as Lateral Flow Immunoassay (LFIA).
- Radioimmunoassay (RIA) is a historical alternative to ELISA for conducting immunoassays.
Direct ELISA
- A major disadvantage of the direct ELISA method is that it can be less sensitive than other ELISA methods.
- The purpose of linking the enzyme to an appropriate antibody is to enable detection of the antigen-antibody reaction.
- The direct ELISA method involves using a spectrometer to measure the signal produced by the enzyme-substrate reaction.
ELISA Variations
- The indirect ELISA method involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well, a process known as blocking.
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