11 Questions
Which of the following is true about ELISA?
ELISA is a type of enzyme immunoassay used to detect the presence of a ligand in a liquid sample.
What is the purpose of immobilizing the antigen in an ELISA?
To allow the antigen to bind with the detection antibody.
What is the role of the detection antibody in an ELISA?
To form a complex with the antigen.
Which of the following is true about ELISA plates?
ELISA plates have the reaction products immunosorbed on the solid phase
What is the purpose of adding an enzymatic substrate in ELISA?
To generate a signal that can be quantified
What is the name of the technique that uses dry strips for analysis?
Reflectometry
What is the historical alternative to ELISA for conducting immunoassays?
Radioimmunoassay
Which of the following is a major disadvantage of the direct ELISA method?
The antigen immobilization is not specific
What is the purpose of linking the enzyme to an appropriate antibody in the ELISA technique?
To amplify the color change
Which type of ELISA involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well?
Direct ELISA
What is the purpose of using a spectrometer in the direct ELISA method?
To give quantitative values for color strength
Study Notes
ELISA Basics
- ELISA (Enzyme-Linked Immunosorbent Assay) is a technique used for immunoassays.
- ELISA involves immobilizing the antigen to facilitate the reaction.
ELISA Process
- The detection antibody plays a crucial role in ELISA by binding to the antigen, allowing for detection.
- ELISA plates are used to facilitate the reaction and are typically 96-well microtiter plates.
- An enzymatic substrate is added to ELISA to enable the detection of the antigen-antibody reaction.
- The enzyme linked to the antibody catalyzes the substrate, producing a measurable signal.
Other Techniques
- The dry strip analysis technique is known as Lateral Flow Immunoassay (LFIA).
- Radioimmunoassay (RIA) is a historical alternative to ELISA for conducting immunoassays.
Direct ELISA
- A major disadvantage of the direct ELISA method is that it can be less sensitive than other ELISA methods.
- The purpose of linking the enzyme to an appropriate antibody is to enable detection of the antigen-antibody reaction.
- The direct ELISA method involves using a spectrometer to measure the signal produced by the enzyme-substrate reaction.
ELISA Variations
- The indirect ELISA method involves adding a solution of nonreacting protein to cover any uncoated plastic surface in the well, a process known as blocking.
Test your knowledge on ELISA - a commonly used analytical biochemistry assay that detects the presence of proteins in liquid samples using antibodies. Learn about its history, principles, and applications in this informative quiz.
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