E. Histolytica and E. Coli Characteristics
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Questions and Answers

The learning outcome of this course is to understand the features and ______ of protozoa.

life cycle

The objective of this course is to classify protozoa into different ______.

phyla

The subphylum Sarcodina includes the genus ______.

Amoeba

The protozoan Entamoeba histolytica is found in the ______.

<p>colon</p> Signup and view all the answers

The protozoan Giardia lamblia is found in the ______ intestine.

<p>small</p> Signup and view all the answers

Giardia lamblia has a ______ shape.

<p>pear</p> Signup and view all the answers

Giardia lamblia has ______ flagella.

<p>8</p> Signup and view all the answers

The transmission of Giardia lamblia occurs through the ______ route.

<p>fecal-oral</p> Signup and view all the answers

Giardia lamblia belongs to the phylum ______.

<p>Sarcomastigophora</p> Signup and view all the answers

Giardia lamblia has ______ nucleus.

<p>2</p> Signup and view all the answers

The diameter of the Giardia lamblia is about ______ mm.

<p>8-12</p> Signup and view all the answers

The nucleus of Giardia lamblia is ______ and has a small eccentric karyosome.

<p>four</p> Signup and view all the answers

The Giardia lamblia cyst has a clear space beneath the ______ cyst wall.

<p>thin</p> Signup and view all the answers

Infection of Giardia lamblia occurs by the ingestion of ______ in contaminated water, food, or by the fecal-oral route.

<p>cysts</p> Signup and view all the answers

The process of ______ releases trophozoites in the small intestine.

<p>excystation</p> Signup and view all the answers

Giardia lamblia can be detected in a ______ specimen

<p>stool</p> Signup and view all the answers

Trichomonas vaginalis is transmitted through ______ contact

<p>sexual</p> Signup and view all the answers

Kohn staining is used for the detection of ______ cysts

<p>Giardia</p> Signup and view all the answers

Trichomonas vaginalis has ______ anterior flagella

<p>4</p> Signup and view all the answers

Giardia lamblia has a ______ motility

<p>graceful falling-leaf</p> Signup and view all the answers

The parasitic form called ______ is sensitive to environmental changes and it disintegrates rapidly upon release from the body.

<p>trophozoite</p> Signup and view all the answers

The morphological forms of Naegleria fowleri can be _______, ______ and ______.

<p>amoeboflagellate, amoebic, flagellate</p> Signup and view all the answers

Peripheral nuclear chromatin is absent in ______ and ______.

<p>E.histolytica, E.coli</p> Signup and view all the answers

Free living amoeba include ______ and ______.

<p>Naegleria, Acanthamoeba</p> Signup and view all the answers

Diseases caused by Acanthamoeba spp. are ______ and ______.

<p>meningoencephalitis, keratitis</p> Signup and view all the answers

Study Notes

Protozoa of Medical Importance

  • Protozoa are divided into four phyla: Sarcomastigophora, Ciliophora, Apicomplexa, and Microspora.

Subphylum Sarcodina (= Amoeba)

  • Found in colon, liver, and lungs.
  • Examples include:
  • Entamoeba histolytica
  • Entamoeba coli
  • Endolimax nana
  • Iodamoeba butschlii
  • Blastocystis hominis

Subphylum Mastigophora (= Flagellates)

  • Found in small intestine.
  • Examples include:
  • Giardia lamblia
  • Trichomonas vaginalis
  • Dientamoeba fragilis

Giardia lamblia

  • Found in lakes, streams, and other water sources worldwide.
  • Transmission occurs through the fecal-oral route, primarily through contaminated food and water.
  • Characteristics:
  • Pear-shaped with 8 flagella.
  • Length: 9-21 mm, width: 5-15 mm.
  • Bilaterally symmetrical with 2 nuclei, one on either side of the axostyle.
  • Fine granular cytoplasm with 2 median parabasal bodies.
  • Life cycle:
  • Excystation occurs in the small intestine, releasing trophozoites.
  • Trophozoites multiply by longitudinal binary fission, remaining in the lumen of the proximal small bowel.
  • Encystation occurs as the parasites transit towards the colon.
  • Cysts are the infectious stage, found commonly in non-diarrheal feces.

Diagnostic Techniques for Giardia lamblia

  • Examination of triple fecal tests from non-consecutive days.
  • Concentration technique and permanent stain smear.
  • Stool specimen examination using Kohn staining and trichrome staining.

Trichomonas vaginalis

  • Found in the vagina and urethra.
  • Distribution: worldwide, with higher prevalence among persons with multiple sexual partners.
  • Transmission occurs through:
  • Sexual contact.
  • Contaminated towels and examination equipment.
  • Infants may be infected during birth.
  • Characteristics:
  • Pear-shaped with 3-5 anterior flagella and an undulating membrane.
  • Length: 10-30 mm, width: 5-20 mm.
  • Ovoid nucleus at the anterior end.
  • Axostyle from anterior to posterior, ending like a tail.
  • In males, it infects the urethra, seminal vesicle, and prostate, causing urethritis.
  • In females, it infects the vulva, vagina, and cervix, causing vaginitis.
  • Treatment: metronidazole or quinacrine.

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Test your knowledge of E. Histolytica and E. Coli characteristics, including their life cycles, nucleus, and infection symptoms.

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