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Questions and Answers
The learning outcome of this course is to understand the features and ______ of protozoa.
The learning outcome of this course is to understand the features and ______ of protozoa.
life cycle
The objective of this course is to classify protozoa into different ______.
The objective of this course is to classify protozoa into different ______.
phyla
The subphylum Sarcodina includes the genus ______.
The subphylum Sarcodina includes the genus ______.
Amoeba
The protozoan Entamoeba histolytica is found in the ______.
The protozoan Entamoeba histolytica is found in the ______.
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The protozoan Giardia lamblia is found in the ______ intestine.
The protozoan Giardia lamblia is found in the ______ intestine.
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Giardia lamblia has a ______ shape.
Giardia lamblia has a ______ shape.
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Giardia lamblia has ______ flagella.
Giardia lamblia has ______ flagella.
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The transmission of Giardia lamblia occurs through the ______ route.
The transmission of Giardia lamblia occurs through the ______ route.
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Giardia lamblia belongs to the phylum ______.
Giardia lamblia belongs to the phylum ______.
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Giardia lamblia has ______ nucleus.
Giardia lamblia has ______ nucleus.
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The diameter of the Giardia lamblia is about ______ mm.
The diameter of the Giardia lamblia is about ______ mm.
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The nucleus of Giardia lamblia is ______ and has a small eccentric karyosome.
The nucleus of Giardia lamblia is ______ and has a small eccentric karyosome.
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The Giardia lamblia cyst has a clear space beneath the ______ cyst wall.
The Giardia lamblia cyst has a clear space beneath the ______ cyst wall.
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Infection of Giardia lamblia occurs by the ingestion of ______ in contaminated water, food, or by the fecal-oral route.
Infection of Giardia lamblia occurs by the ingestion of ______ in contaminated water, food, or by the fecal-oral route.
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The process of ______ releases trophozoites in the small intestine.
The process of ______ releases trophozoites in the small intestine.
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Giardia lamblia can be detected in a ______ specimen
Giardia lamblia can be detected in a ______ specimen
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Trichomonas vaginalis is transmitted through ______ contact
Trichomonas vaginalis is transmitted through ______ contact
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Kohn staining is used for the detection of ______ cysts
Kohn staining is used for the detection of ______ cysts
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Trichomonas vaginalis has ______ anterior flagella
Trichomonas vaginalis has ______ anterior flagella
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Giardia lamblia has a ______ motility
Giardia lamblia has a ______ motility
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The parasitic form called ______ is sensitive to environmental changes and it disintegrates rapidly upon release from the body.
The parasitic form called ______ is sensitive to environmental changes and it disintegrates rapidly upon release from the body.
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The morphological forms of Naegleria fowleri can be _______, ______ and ______.
The morphological forms of Naegleria fowleri can be _______, ______ and ______.
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Peripheral nuclear chromatin is absent in ______ and ______.
Peripheral nuclear chromatin is absent in ______ and ______.
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Free living amoeba include ______ and ______.
Free living amoeba include ______ and ______.
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Diseases caused by Acanthamoeba spp. are ______ and ______.
Diseases caused by Acanthamoeba spp. are ______ and ______.
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Study Notes
Protozoa of Medical Importance
- Protozoa are divided into four phyla: Sarcomastigophora, Ciliophora, Apicomplexa, and Microspora.
Subphylum Sarcodina (= Amoeba)
- Found in colon, liver, and lungs.
- Examples include:
- Entamoeba histolytica
- Entamoeba coli
- Endolimax nana
- Iodamoeba butschlii
- Blastocystis hominis
Subphylum Mastigophora (= Flagellates)
- Found in small intestine.
- Examples include:
- Giardia lamblia
- Trichomonas vaginalis
- Dientamoeba fragilis
Giardia lamblia
- Found in lakes, streams, and other water sources worldwide.
- Transmission occurs through the fecal-oral route, primarily through contaminated food and water.
- Characteristics:
- Pear-shaped with 8 flagella.
- Length: 9-21 mm, width: 5-15 mm.
- Bilaterally symmetrical with 2 nuclei, one on either side of the axostyle.
- Fine granular cytoplasm with 2 median parabasal bodies.
- Life cycle:
- Excystation occurs in the small intestine, releasing trophozoites.
- Trophozoites multiply by longitudinal binary fission, remaining in the lumen of the proximal small bowel.
- Encystation occurs as the parasites transit towards the colon.
- Cysts are the infectious stage, found commonly in non-diarrheal feces.
Diagnostic Techniques for Giardia lamblia
- Examination of triple fecal tests from non-consecutive days.
- Concentration technique and permanent stain smear.
- Stool specimen examination using Kohn staining and trichrome staining.
Trichomonas vaginalis
- Found in the vagina and urethra.
- Distribution: worldwide, with higher prevalence among persons with multiple sexual partners.
- Transmission occurs through:
- Sexual contact.
- Contaminated towels and examination equipment.
- Infants may be infected during birth.
- Characteristics:
- Pear-shaped with 3-5 anterior flagella and an undulating membrane.
- Length: 10-30 mm, width: 5-20 mm.
- Ovoid nucleus at the anterior end.
- Axostyle from anterior to posterior, ending like a tail.
- In males, it infects the urethra, seminal vesicle, and prostate, causing urethritis.
- In females, it infects the vulva, vagina, and cervix, causing vaginitis.
- Treatment: metronidazole or quinacrine.
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