7 Questions
What is the term used to describe the process when a recombinant DNA is inserted within the coding sequence of an enzyme, resulting in the inactivation of the gene for synthesis of this enzyme?
Insertional inactivation
In the presence of a chromogenic substrate, what color do colonies produce if the plasmid in the bacteria does not have an insert?
Blue
Which pathogen has the ability to deliver a piece of DNA known as 'T-DNA' to transform normal plant cells into a tumor?
Agrobacterium tumifaciens
How do recombinant colonies differ from non-recombinant colonies when a chromogenic substrate is present?
Recombinant colonies do not produce any color
What is the term used to describe the ability of retroviruses in animals to transform normal cells into cancerous cells?
Oncogenic activation
Which gene transfer method forces eukaryotic cells to do what the bacteria or viruses want?
Transformation factor
What piece of DNA is delivered by Agrobacterium tumifaciens to transform normal plant cells into a tumor?
'T-DNA'
Learn about the process of creating recombinant DNA by combining a gene of interest with a vector DNA, followed by amplifying the gene using Polymerase Chain Reaction (PCR). Understand the basic principles behind these techniques.
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