DNA Recombination and PCR Basics
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Questions and Answers

What is the term used to describe the process when a recombinant DNA is inserted within the coding sequence of an enzyme, resulting in the inactivation of the gene for synthesis of this enzyme?

  • Gene inactivation
  • Chromogenic transformation
  • Insertional inactivation (correct)
  • Recombinant transformation
  • In the presence of a chromogenic substrate, what color do colonies produce if the plasmid in the bacteria does not have an insert?

  • Green
  • Yellow
  • Blue (correct)
  • Red
  • Which pathogen has the ability to deliver a piece of DNA known as 'T-DNA' to transform normal plant cells into a tumor?

  • Bacillus anthracis
  • Streptococcus pyogenes
  • Agrobacterium tumifaciens (correct)
  • Escherichia coli
  • How do recombinant colonies differ from non-recombinant colonies when a chromogenic substrate is present?

    <p>Recombinant colonies do not produce any color</p> Signup and view all the answers

    What is the term used to describe the ability of retroviruses in animals to transform normal cells into cancerous cells?

    <p>Oncogenic activation</p> Signup and view all the answers

    Which gene transfer method forces eukaryotic cells to do what the bacteria or viruses want?

    <p>Transformation factor</p> Signup and view all the answers

    What piece of DNA is delivered by Agrobacterium tumifaciens to transform normal plant cells into a tumor?

    <p>'T-DNA'</p> Signup and view all the answers

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