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DNA Manipulative Enzymes Overview

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Questions and Answers

What is the function of DNA ligase in the cell?

To repair single-stranded breaks in double-stranded DNA molecules

What is the dual activity of DNA polymerase I?

DNA polymerization and DNA degradation

What is the significance of the Klenow fragment?

It retains the polymerase function but is unable to degrade DNA

How do restriction endonucleases cleave DNA?

At specific recognition sites on double-stranded DNA Signup and view all the answers

What is the source of Taq DNA polymerase used in PCR?

The bacterium Thermus aquaticus Signup and view all the answers

What is the role of DNA polymerases in synthesizing DNA?

To synthesize a new DNA strand complementary to an existing template Signup and view all the answers

What is the main function of nucleases in DNA manipulation?

Cut, shorten, or degrade nucleic acid molecules Signup and view all the answers

How do exonucleases differ from endonucleases in their activity?

Exonucleases remove nucleotides from the end of a DNA molecule, while endonucleases break internal phosphodiester bonds within a DNA molecule. Signup and view all the answers

Give an example of an exonuclease enzyme and its activity.

Bal31; removes nucleotides from both strands of a double-stranded molecule Signup and view all the answers

What is the function of ligases in DNA manipulation?

Join nucleic acid molecules together Signup and view all the answers

What role do polymerases play in DNA manipulation?

Make copies of molecules Signup and view all the answers

What is the function of modifying enzymes in DNA manipulation?

Remove or add chemical groups Signup and view all the answers