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DNA Manipulation Enzymes Quiz
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DNA Manipulation Enzymes Quiz

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Questions and Answers

What is the primary function of DNAase I?

  • Cleave DNA at sites specified by specific DNA sequences
  • Initiate synthesis from 5’ to 3’
  • Digest double-stranded and single-stranded DNA
  • Cleave DNA at nonspecific cleavage sites (correct)
  • Which enzyme is responsible for making a specific cut in DNA?

  • DNA Polymerase I
  • Mung bean nuclease
  • DNAase I
  • Restriction endonucleases (correct)
  • What is the function of Klenow fragment?

  • Add free nucleotides to the 3’ end of the new strand
  • Initiate synthesis from 5’ to 3’ (correct)
  • Cleave DNA at nonspecific cleavage sites
  • Digest double-stranded and single-stranded DNA
  • Which enzyme possesses inherent 3’ to 5’ and 5’ to 3’ exonuclease activity?

    <p>DNA Polymerase I</p> Signup and view all the answers

    From which source is DNAase I isolated?

    <p>Bovine pancreas</p> Signup and view all the answers

    Study Notes

    Enzymes used in Gene Manipulation

    • Identified and isolated from different bacteria strains, commercially available as highly purified recombinant enzymes.

    Classes of Enzymes used in Gene Manipulation

    • Nucleases: cut or degrade DNA molecules
    • Polymerases: copy or make new strands of DNA
    • Ligases: join pieces of DNA fragments together
    • Modifying enzymes: modify the DNA by adding or removing chemical groups
    • Topoisomerase: remove or introduce supercoils from covalently closed-circular (CCC) DNA

    Nucleases

    • Exonucleases: degrade DNA at either ends
    • Exonuclease III (Exo III): cleaves dsDNA, initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and initiates at nicked sites in double-stranded DNA
    • Catalyzes: the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction
    • Exonuclease VII (Exo VII): cleaves ssDNA

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    Description

    Test your knowledge on the enzymes used in gene manipulation, including nucleases, polymerases, ligases, and modifying enzymes. Learn about their functions and origins from different bacterial strains.

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