Podcast
Questions and Answers
What is the primary function of DNAase I?
What is the primary function of DNAase I?
Which enzyme is responsible for making a specific cut in DNA?
Which enzyme is responsible for making a specific cut in DNA?
What is the function of Klenow fragment?
What is the function of Klenow fragment?
Which enzyme possesses inherent 3’ to 5’ and 5’ to 3’ exonuclease activity?
Which enzyme possesses inherent 3’ to 5’ and 5’ to 3’ exonuclease activity?
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From which source is DNAase I isolated?
From which source is DNAase I isolated?
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Study Notes
Enzymes used in Gene Manipulation
- Identified and isolated from different bacteria strains, commercially available as highly purified recombinant enzymes.
Classes of Enzymes used in Gene Manipulation
- Nucleases: cut or degrade DNA molecules
- Polymerases: copy or make new strands of DNA
- Ligases: join pieces of DNA fragments together
- Modifying enzymes: modify the DNA by adding or removing chemical groups
- Topoisomerase: remove or introduce supercoils from covalently closed-circular (CCC) DNA
Nucleases
- Exonucleases: degrade DNA at either ends
- Exonuclease III (Exo III): cleaves dsDNA, initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and initiates at nicked sites in double-stranded DNA
- Catalyzes: the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction
- Exonuclease VII (Exo VII): cleaves ssDNA
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Description
Test your knowledge on the enzymes used in gene manipulation, including nucleases, polymerases, ligases, and modifying enzymes. Learn about their functions and origins from different bacterial strains.
