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Questions and Answers
What is the main function of restriction endonucleases?
Which enzyme cuts both single and double-stranded DNA molecules?
What does methylation do in the restriction-modification system?
Which enzyme is isolated from the fungus Aspergillus oryzae and cleaves only single strands of DNA?
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What is the role of modifying enzymes associated with restriction endonucleases?
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Which type of nucleases degrade just one strand of a double-stranded DNA molecule?
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What is the distinguishing feature between blunt ends and sticky ends in DNA fragments?
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How many different restriction endonucleases have been isolated according to the text?
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Which source is Deoxyribonuclease I (DNase I) isolated from?
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What is the specificity of DNase I in cutting DNA molecules?
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