10 Questions
What is the main function of restriction endonucleases?
Acting as a natural defense mechanism in bacteria
Which enzyme cuts both single and double-stranded DNA molecules?
Deoxyribonuclease I
What does methylation do in the restriction-modification system?
Blocks the action of degradative enzymes
Which enzyme is isolated from the fungus Aspergillus oryzae and cleaves only single strands of DNA?
S1 endonuclease
What is the role of modifying enzymes associated with restriction endonucleases?
Methylate specific bases in host DNA
Which type of nucleases degrade just one strand of a double-stranded DNA molecule?
S1 endonuclease
What is the distinguishing feature between blunt ends and sticky ends in DNA fragments?
Sticky ends have overhangs while blunt ends do not.
How many different restriction endonucleases have been isolated according to the text?
Around 3000
Which source is Deoxyribonuclease I (DNase I) isolated from?
Cow pancreas
What is the specificity of DNase I in cutting DNA molecules?
Cuts at any internal phosphodiester bond in DNA
Learn about the tools of molecular biology for manipulating DNA, including nucleases for cutting and degrading nucleic acids, ligases for joining molecules, polymerases for making copies, and modifying enzymes for adding or removing chemical groups. This quiz is based on the book 'Clinical Microbiology-2021' by Dr. Sarfraz A. Tunio.
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