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Questions and Answers
What is the main function of restriction endonucleases?
What is the main function of restriction endonucleases?
- Synthesizing new DNA strands
- Initiating DNA replication
- Repairing DNA damage
- Acting as a natural defense mechanism in bacteria (correct)
Which enzyme cuts both single and double-stranded DNA molecules?
Which enzyme cuts both single and double-stranded DNA molecules?
- DNase I
- Deoxyribonuclease I (correct)
- Restriction endonuclease
- S1 endonuclease
What does methylation do in the restriction-modification system?
What does methylation do in the restriction-modification system?
- Blocks the action of degradative enzymes (correct)
- Repairs damaged DNA strands
- Promotes degradation of foreign DNA
- Facilitates the binding of restriction enzymes
Which enzyme is isolated from the fungus Aspergillus oryzae and cleaves only single strands of DNA?
Which enzyme is isolated from the fungus Aspergillus oryzae and cleaves only single strands of DNA?
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What is the role of modifying enzymes associated with restriction endonucleases?
What is the role of modifying enzymes associated with restriction endonucleases?
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Which type of nucleases degrade just one strand of a double-stranded DNA molecule?
Which type of nucleases degrade just one strand of a double-stranded DNA molecule?
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What is the distinguishing feature between blunt ends and sticky ends in DNA fragments?
What is the distinguishing feature between blunt ends and sticky ends in DNA fragments?
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How many different restriction endonucleases have been isolated according to the text?
How many different restriction endonucleases have been isolated according to the text?
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Which source is Deoxyribonuclease I (DNase I) isolated from?
Which source is Deoxyribonuclease I (DNase I) isolated from?
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What is the specificity of DNase I in cutting DNA molecules?
What is the specificity of DNase I in cutting DNA molecules?
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