Podcast
Questions and Answers
What is the primary role of counter immunoelectrophoresis (CIE)?
What is the primary role of counter immunoelectrophoresis (CIE)?
What happens to the antigen and antibody during the CIE process?
What happens to the antigen and antibody during the CIE process?
How long does it typically take to observe a precipitin reaction in CIE?
How long does it typically take to observe a precipitin reaction in CIE?
What does a precipitin line between the antigen and antisera wells signify?
What does a precipitin line between the antigen and antisera wells signify?
Signup and view all the answers
Which of the following is a limitation of counter immunoelectrophoresis?
Which of the following is a limitation of counter immunoelectrophoresis?
Signup and view all the answers
What does the presence of multiple precipitin lines suggest?
What does the presence of multiple precipitin lines suggest?
Signup and view all the answers
Which of the following fluids can CIE be used to analyze?
Which of the following fluids can CIE be used to analyze?
Signup and view all the answers
What is a key advantage of CIE compared to double immunodiffusion?
What is a key advantage of CIE compared to double immunodiffusion?
Signup and view all the answers
Which of the following antigens is commonly detected using CIE?
Which of the following antigens is commonly detected using CIE?
Signup and view all the answers
In CIE, what type of gel medium is typically used for the electrophoretic process?
In CIE, what type of gel medium is typically used for the electrophoretic process?
Signup and view all the answers
Study Notes
Counter Immunoelectrophoresis (CIE)
- CIE is a laboratory technique used to detect the binding of an antibody to its specific antigen
- An electric field speeds up the migration of antigens and antibodies in a gel medium (usually agar)
- Antibodies and antigens migrate towards one another, forming a line of precipitation
- The principle involves the movement of negatively charged antigens towards the anode and antibodies towards the cathode through the agar gel
- The test is performed on a glass slide or plate with agarose gel, and wells are punched out to contain the antigen and antibody
- Applying an electric current facilitates the migration of antigens and antibodies
- The line of precipitation (precipitin lines) appears within 30-60 minutes, indicating a positive reaction
Interpretation
- A precipitin line between the antigen and antisera wells indicates a positive reaction (specific antigen-antibody reaction) due to the presence of the specific antibody
- Absence of the precipitin line indicates no reaction or the lack of the specific antibody
- Multiple precipitin lines suggest the heterogeneity of the antibody for the antigen
Advantages and Limitations
- Advantages: Faster and more sensitive than double immunodiffusion (takes about 30 minutes)
- Limitations: More expensive than agglutination-based tests, and possibly lower sensitivity, speed, and simplicity compared to latex agglutination tests, requiring large quantities of antigens and antibodies
Uses
- Used for rapid and specific detection of antigens and antibodies in serum, cerebrospinal fluid, and other body fluids for diagnosing various infectious diseases (bacterial, viral, fungal, parasitic)
- Commonly used for detecting Hepatitis B surface antigen (HBsAg), fetoprotein, hydatid and amoebic antigens in serum
- Useful in detecting pneumococcal capsular antigens in sputum
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
This quiz covers the Counter Immunoelectrophoresis (CIE) technique, a laboratory method for detecting antibody-antigen binding. Topics include the principles of movement within the gel medium, the formation of precipitin lines, and test interpretation. Ideal for students and professionals in immunology and laboratory sciences.
