Podcast
Questions and Answers
In column chromatography, what are the two phases involved?
In column chromatography, what are the two phases involved?
- Solid (stationary phase) and liquid (moving phase) (correct)
- Gas (moving phase) and liquid (stationary phase)
- Solid (moving phase) and liquid (stationary phase)
- Gas (stationary phase) and liquid (moving phase)
What is the main advantage of column chromatography?
What is the main advantage of column chromatography?
- High resolution and fast separation
- Minimal use of solvents
- Relatively low cost and disposability of the stationary phase (correct)
- Ability to separate gases and liquids
What is the role of the eluent in column chromatography?
What is the role of the eluent in column chromatography?
- It dissolves the sample and carries it through the column (correct)
- It remains stationary to facilitate separation
- It evaporates to leave the separated components
- It reacts with the stationary phase to separate the components
What is the principle behind the separation in column chromatography?
What is the principle behind the separation in column chromatography?
What is the scale of applications for which column chromatography is often used?
What is the scale of applications for which column chromatography is often used?
Column chromatography is a solid-liquid technique in which the two phases are a solid (stationary phase) and a ______ (moving phase).
Column chromatography is a solid-liquid technique in which the two phases are a solid (stationary phase) and a ______ (moving phase).
The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to ______.
The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to ______.
Column chromatography is often used for preparative applications on scales from micrograms up to ______.
Column chromatography is often used for preparative applications on scales from micrograms up to ______.
When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates. Those with lower affinity to stationary phase move faster and eluted out first while those with greater affinity move or travel slower and get eluted out ______.
When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates. Those with lower affinity to stationary phase move faster and eluted out first while those with greater affinity move or travel slower and get eluted out ______.
The sample is dissolved in a small quantity of solvent (the eluent) and applied to the top of the column. The eluent, instead of rising by capillary action up a thin layer, flows down through the column filled with the ______.
The sample is dissolved in a small quantity of solvent (the eluent) and applied to the top of the column. The eluent, instead of rising by capillary action up a thin layer, flows down through the column filled with the ______.
