10 Questions
In column chromatography, what are the two phases involved?
Solid (stationary phase) and liquid (moving phase)
What is the main advantage of column chromatography?
Relatively low cost and disposability of the stationary phase
What is the role of the eluent in column chromatography?
It dissolves the sample and carries it through the column
What is the principle behind the separation in column chromatography?
Individual components of the mixture move with different rates based on their affinity to the stationary phase
What is the scale of applications for which column chromatography is often used?
From micrograms up to kilograms
Column chromatography is a solid-liquid technique in which the two phases are a solid (stationary phase) and a ______ (moving phase).
liquid
The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to ______.
recycling
Column chromatography is often used for preparative applications on scales from micrograms up to ______.
kilograms
When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates. Those with lower affinity to stationary phase move faster and eluted out first while those with greater affinity move or travel slower and get eluted out ______.
last
The sample is dissolved in a small quantity of solvent (the eluent) and applied to the top of the column. The eluent, instead of rising by capillary action up a thin layer, flows down through the column filled with the ______.
adsorbent
Test your knowledge of column chromatography with this quiz! Challenge yourself with questions about the principles, techniques, and applications of this versatile method for separating and purifying substances in laboratory experiments.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
