Column Chromatography in Chemistry
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Questions and Answers

What is the primary principle of column chromatography?

  • Differential density of compounds
  • Differential solubility of compounds
  • Differential adsorption of compounds to the adsorbent (correct)
  • Differential viscosity of compounds
  • What is the purpose of introducing the mobile phase from the top of the column?

  • To mix the components of the mixture
  • To separate the components of the mixture (correct)
  • To increase the adsorption of the solute molecules
  • To slow down the movement of the components
  • What is the retardation factor (Rf) in column chromatography?

  • The rate of movement of the solvent
  • The ratio of the distance travelled by the solvent to the distance travelled by the solute
  • The rate of adsorption of solute molecules
  • The ratio of the distance travelled by the solute to the distance travelled by the solvent (correct)
  • What is the characteristic of the adsorption of solute molecules to the column in column chromatography?

    <p>Reversible</p> Signup and view all the answers

    What is the final result of the separation process in column chromatography?

    <p>Separated fractions of the original mixture</p> Signup and view all the answers

    What is the basis of separation of substances in column chromatography?

    <p>Differential adsorption of compounds to the adsorbent</p> Signup and view all the answers

    What is the formula to express the rate of movement of components in column chromatography?

    <p>Rf = distance travelled by solute/ distance travelled by solvent</p> Signup and view all the answers

    What is the type of chromatography technique used in column chromatography?

    <p>Adsorption chromatography</p> Signup and view all the answers

    What happens to the components that move slowly in column chromatography?

    <p>They are eluted out last</p> Signup and view all the answers

    What is the nature of adsorption of solute molecules to the column in column chromatography?

    <p>Reversible</p> Signup and view all the answers

    Study Notes

    Column Chromatography

    • A technique used to separate a single chemical compound from a mixture dissolved in a fluid
    • Separates substances based on differential adsorption of compounds to the adsorbent as they move through the column at different rates
    • Can be used on small and large scales to purify materials for future experiments
    • A type of adsorption chromatography technique

    Principle

    • When the mobile phase and mixture are introduced into the column, individual components move at different rates
    • Components with lower adsorption and affinity to stationary phase travel faster
    • Components with greater adsorption and affinity to stationary phase travel slower
    • Faster-moving components are removed first, while slower-moving components are eluted last
    • Adsorption of solute molecules to the column occurs in a reversible manner
    • Rate of movement expressed as: Rf = distance travelled by solute / distance travelled by solvent

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    Description

    Test your understanding of column chromatography, a technique used to separate and purify chemical compounds from a mixture. This quiz covers the principles and applications of column chromatography in chemistry.

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