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Questions and Answers
What is the primary principle of column chromatography?
What is the primary principle of column chromatography?
- Differential density of compounds
- Differential solubility of compounds
- Differential adsorption of compounds to the adsorbent (correct)
- Differential viscosity of compounds
What is the purpose of introducing the mobile phase from the top of the column?
What is the purpose of introducing the mobile phase from the top of the column?
- To mix the components of the mixture
- To separate the components of the mixture (correct)
- To increase the adsorption of the solute molecules
- To slow down the movement of the components
What is the retardation factor (Rf) in column chromatography?
What is the retardation factor (Rf) in column chromatography?
- The rate of movement of the solvent
- The ratio of the distance travelled by the solvent to the distance travelled by the solute
- The rate of adsorption of solute molecules
- The ratio of the distance travelled by the solute to the distance travelled by the solvent (correct)
What is the characteristic of the adsorption of solute molecules to the column in column chromatography?
What is the characteristic of the adsorption of solute molecules to the column in column chromatography?
What is the final result of the separation process in column chromatography?
What is the final result of the separation process in column chromatography?
What is the basis of separation of substances in column chromatography?
What is the basis of separation of substances in column chromatography?
What is the formula to express the rate of movement of components in column chromatography?
What is the formula to express the rate of movement of components in column chromatography?
What is the type of chromatography technique used in column chromatography?
What is the type of chromatography technique used in column chromatography?
What happens to the components that move slowly in column chromatography?
What happens to the components that move slowly in column chromatography?
What is the nature of adsorption of solute molecules to the column in column chromatography?
What is the nature of adsorption of solute molecules to the column in column chromatography?
Study Notes
Column Chromatography
- A technique used to separate a single chemical compound from a mixture dissolved in a fluid
- Separates substances based on differential adsorption of compounds to the adsorbent as they move through the column at different rates
- Can be used on small and large scales to purify materials for future experiments
- A type of adsorption chromatography technique
Principle
- When the mobile phase and mixture are introduced into the column, individual components move at different rates
- Components with lower adsorption and affinity to stationary phase travel faster
- Components with greater adsorption and affinity to stationary phase travel slower
- Faster-moving components are removed first, while slower-moving components are eluted last
- Adsorption of solute molecules to the column occurs in a reversible manner
- Rate of movement expressed as: Rf = distance travelled by solute / distance travelled by solvent
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Description
Test your understanding of column chromatography, a technique used to separate and purify chemical compounds from a mixture. This quiz covers the principles and applications of column chromatography in chemistry.
