Clinical BioChemistry 2: Enzymes Overview
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Questions and Answers

What suffix is attached to a description of the chemical reaction catalyzed by an enzyme?

  • -ase (correct)
  • -zyme
  • -reactase
  • -catalyse
  • What is the role of the active site in an enzyme molecule?

  • To convert the substrate to product
  • To increase the velocity of the reaction
  • To participate in substrate binding and catalysis (correct)
  • To bind the product
  • What is the result of the binding of the substrate to the enzyme?

  • A conformational change in the enzyme (correct)
  • The formation of a new product
  • The inhibition of the reaction
  • A conformational change in the substrate
  • What is the term for the number of molecules of substrate converted to product per enzyme molecule per second?

    <p>Turnover number</p> Signup and view all the answers

    What is a characteristic of enzyme-catalyzed reactions?

    <p>They are highly specific</p> Signup and view all the answers

    What is the term for RNAs with catalytic activity?

    <p>Ribozymes</p> Signup and view all the answers

    What is the term for an active enzyme with its nonprotein component?

    <p>Holoenzyme</p> Signup and view all the answers

    Which type of molecule is a cofactor?

    <p>Metal ion</p> Signup and view all the answers

    What is the purpose of compartmentalization of enzymes in specific organelles within the cell?

    <p>To isolate the reaction substrate or product from other competing reactions</p> Signup and view all the answers

    What is the term for the energy required to convert a substance from ground state to transition state?

    <p>Activation energy</p> Signup and view all the answers

    What is the role of a catalyst in a chemical reaction?

    <p>It accelerates the reaction</p> Signup and view all the answers

    How does the rate of an enzyme-catalyzed reaction change with an increase in substrate concentration?

    <p>It increases gradually within a limited range</p> Signup and view all the answers

    What is the term for a small organic molecule that is required for enzymatic activity and transiently associates with the enzyme?

    <p>Cosubstrate</p> Signup and view all the answers

    What is the term for the increase in enzyme velocity when the temperature is increased by 10°C?

    <p>Q10</p> Signup and view all the answers

    What is the theory that explains the action of catalysts?

    <p>Transition state theory</p> Signup and view all the answers

    What is the typical temperature range for optimal enzyme activity?

    <p>35°C-40°C</p> Signup and view all the answers

    Why do some enzymes, like Taq DNA polymerase, remain active at high temperatures?

    <p>Due to their very stable structure and conformation</p> Signup and view all the answers

    How does the velocity of an enzyme reaction change with an increase in temperature?

    <p>It increases gradually and then declines</p> Signup and view all the answers

    At what pH does the enzyme pepsin in the stomach work best?

    <p>pH 2</p> Signup and view all the answers

    What happens to enzyme activity when the product concentration increases?

    <p>It decreases</p> Signup and view all the answers

    What is the effect of ultraviolet radiation on certain enzymes?

    <p>It inhibits them</p> Signup and view all the answers

    What is the Michaelis constant (Km) representative of?

    <p>The enzyme's affinity for its substrate</p> Signup and view all the answers

    What is the typical pH range at which most enzymes function?

    <p>pH 6 to 8</p> Signup and view all the answers

    What does a low value of Km indicate?

    <p>Strong substrate-enzyme affinity</p> Signup and view all the answers

    What is the primary function of enzymes in the body?

    <p>To channel reactants into useful pathways</p> Signup and view all the answers

    What suffix is commonly attached to the substrate of the reaction in enzyme names?

    <p>-ase</p> Signup and view all the answers

    How many major classes of enzymes are there in the systematic naming system?

    <p>6</p> Signup and view all the answers

    What is the purpose of assigning two names to each enzyme?

    <p>To identify enzymes without ambiguity</p> Signup and view all the answers

    What is the role of enzymes in biological reactions?

    <p>To increase the rate of reactions</p> Signup and view all the answers

    What is the term for enzymes that retain their original trivial names?

    <p>Trivial names</p> Signup and view all the answers

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