Cell Biology Quiz Week 7
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Questions and Answers

The cisternal maturation model proposes that protein cargo goes from compartment to compartment in a cis-to-trans face direction via vesicles.

False

It is likely that UGGT has a KDEL sequence.

True

The Golgi apparatus is composed of multiple individual compartments called 'cristae.'

False

The diameter of most cells is measured in units of...

<p>micrometres</p> Signup and view all the answers

You observe that a biomolecule contains hydrocarbon rings. This is most likely a(n)...

<p>steroid</p> Signup and view all the answers

What is the role of the lysosome, and what type of enzyme is found in this organelle?

<p>Intracellular digestion | Hydrolytic enzymes</p> Signup and view all the answers

During lactate fermentation, pyruvate is reduced to regenerate NAD+. ATP is produced directly during this one-step reaction as a by-product.

<p>False</p> Signup and view all the answers

Which statement is true regarding the signal hypothesis?

<p>A signal recognition particle, composed of rRNA and six polypeptides, binds to an amino acid sequence and temporarily halts translation until the complex docks on the ER membrane.</p> Signup and view all the answers

Which of the following is a component of the endomembrane system?

<p>All of the above</p> Signup and view all the answers

ER-bound ribosomes and mitochondrial ribosomes are structurally identical and interchangeable.

<p>False</p> Signup and view all the answers

Ribosomal proteins contain oligosaccharide nuclear localization sequences (NLS) that permit binding to imprints.

<p>False</p> Signup and view all the answers

Microsomes are...

<p>small vesicles formed from cell homogenization and are representative of intact membranous organelles.</p> Signup and view all the answers

You have discovered a stain that can be used to detect glycosylated proteins. If you use this stain on Ptk2 cells, which organelle would you expect to be most intensely stained?

<p>Golgi</p> Signup and view all the answers

A vesicle has a phospholipid called "PLq" in its exoplasmic leaflet. When this vesicle fuses with the plasma membrane, where will PLq be located?

<p>In the exoplasmic leaflet</p> Signup and view all the answers

Which of the following is NOT typically associated with studying the endomembrane system?

<p>FRAP</p> Signup and view all the answers

Study Notes

Cisternal Maturation Model

  • Protein cargo moves from compartment to compartment in a cis-to-trans face direction via vesicles.

Golgi Apparatus

  • Composed of multiple individual compartments called 'cristae.'

Biomolecules

  • A biomolecule containing hydrocarbon rings is likely a steroid.

Cell Size

  • The diameter of most cells is measured in units of micrometers (μm).

Lysosome

  • The role of the lysosome is to break down and recycle cellular waste and foreign substances.
  • Lysosomes contain acid hydrolase enzymes.

Lactate Fermentation

  • During lactate fermentation, pyruvate is reduced to regenerate NAD+.
  • ATP is produced directly as a by-product of this one-step reaction.

Signal Hypothesis

  • The signal hypothesis proposes that the signal sequence of a protein targets it to the ER for translation.

Endomembrane System

  • The endomembrane system consists of the ER, Golgi apparatus, lysosomes, and plasma membrane.
  • ER-bound ribosomes are not structurally identical and interchangeable with mitochondrial ribosomes.

Ribosomal Proteins

  • Ribosomal proteins do not contain oligosaccharide nuclear localization sequences (NLS) that permit binding to imprints.

Microsomes

  • Microsomes are vesicles formed from the ER during cell fractionation.

Glycosylated Proteins

  • Glycosylated proteins can be detected using a specific stain.
  • The stain would most intensely stain the Golgi apparatus in Ptk2 cells.

Vesicle Fusion

  • When a vesicle fuses with the plasma membrane, the phospholipid "PLq" from the exoplasmic leaflet will be located on the outer leaflet of the plasma membrane.

Endomembrane System Studies

  • Electron microscopy is typically associated with studying the endomembrane system.

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Test your knowledge of the cisternal maturation model which suggests that protein cargo moves from one compartment to another in a cis-to-trans face direction using vesicles for transport.

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