15 Questions
The cisternal maturation model proposes that protein cargo goes from compartment to compartment in a cis-to-trans face direction via vesicles.
False
It is likely that UGGT has a KDEL sequence.
True
The Golgi apparatus is composed of multiple individual compartments called 'cristae.'
False
The diameter of most cells is measured in units of...
micrometres
You observe that a biomolecule contains hydrocarbon rings. This is most likely a(n)...
steroid
What is the role of the lysosome, and what type of enzyme is found in this organelle?
Intracellular digestion | Hydrolytic enzymes
During lactate fermentation, pyruvate is reduced to regenerate NAD+. ATP is produced directly during this one-step reaction as a by-product.
False
Which statement is true regarding the signal hypothesis?
A signal recognition particle, composed of rRNA and six polypeptides, binds to an amino acid sequence and temporarily halts translation until the complex docks on the ER membrane.
Which of the following is a component of the endomembrane system?
All of the above
ER-bound ribosomes and mitochondrial ribosomes are structurally identical and interchangeable.
False
Ribosomal proteins contain oligosaccharide nuclear localization sequences (NLS) that permit binding to imprints.
False
Microsomes are...
small vesicles formed from cell homogenization and are representative of intact membranous organelles.
You have discovered a stain that can be used to detect glycosylated proteins. If you use this stain on Ptk2 cells, which organelle would you expect to be most intensely stained?
Golgi
A vesicle has a phospholipid called "PLq" in its exoplasmic leaflet. When this vesicle fuses with the plasma membrane, where will PLq be located?
In the exoplasmic leaflet
Which of the following is NOT typically associated with studying the endomembrane system?
FRAP
Study Notes
Cisternal Maturation Model
- Protein cargo moves from compartment to compartment in a cis-to-trans face direction via vesicles.
Golgi Apparatus
- Composed of multiple individual compartments called 'cristae.'
Biomolecules
- A biomolecule containing hydrocarbon rings is likely a steroid.
Cell Size
- The diameter of most cells is measured in units of micrometers (μm).
Lysosome
- The role of the lysosome is to break down and recycle cellular waste and foreign substances.
- Lysosomes contain acid hydrolase enzymes.
Lactate Fermentation
- During lactate fermentation, pyruvate is reduced to regenerate NAD+.
- ATP is produced directly as a by-product of this one-step reaction.
Signal Hypothesis
- The signal hypothesis proposes that the signal sequence of a protein targets it to the ER for translation.
Endomembrane System
- The endomembrane system consists of the ER, Golgi apparatus, lysosomes, and plasma membrane.
- ER-bound ribosomes are not structurally identical and interchangeable with mitochondrial ribosomes.
Ribosomal Proteins
- Ribosomal proteins do not contain oligosaccharide nuclear localization sequences (NLS) that permit binding to imprints.
Microsomes
- Microsomes are vesicles formed from the ER during cell fractionation.
Glycosylated Proteins
- Glycosylated proteins can be detected using a specific stain.
- The stain would most intensely stain the Golgi apparatus in Ptk2 cells.
Vesicle Fusion
- When a vesicle fuses with the plasma membrane, the phospholipid "PLq" from the exoplasmic leaflet will be located on the outer leaflet of the plasma membrane.
Endomembrane System Studies
- Electron microscopy is typically associated with studying the endomembrane system.
Test your knowledge of the cisternal maturation model which suggests that protein cargo moves from one compartment to another in a cis-to-trans face direction using vesicles for transport.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
