16 Questions
What is the difference between medical biochemistry and clinical biochemistry?
Medical Biochemistry deals with the chemical basis of the human body, while Clinical Biochemistry deals with clinical diseases/pathological conditions of the human body.
What is the practical module of Clinical Biochemistry going to cover?
The practical module will cover subjects such as spectrophotometry, determination of serum glucose, serum urea, serum creatinine, serum bilirubin, AST and ALT, serum TG, serum cholesterol, ALP, CPK, amylase, and LDL/HDL cholesterol test.
What is the purpose of spectrophotometry in Clinical Biochemistry?
The purpose of spectrophotometry is to determine the Lambda Max of substances and to measure the concentration of substances in solution using light.
What is the fundamental principle of photometric measurement in clinical biochemistry?
The fundamental principle of photometric measurement is the use of light for the measurement of the concentration of substances in solution.
What will be determined in the Practical Week #3 of Clinical Biochemistry?
In Practical Week #3, the determination of Serum Glucose will be covered.
What will be the subject of focus in Practical Week #12 of Clinical Biochemistry?
The subject of focus in Practical Week #12 will be the determination of amylase.
What is the phenomenon called when a certain material absorbs some colors from white light and reflects its disliked color, making it visible to our eyes?
Absorption of light
What is the narrow range of electromagnetic waves with a wavelength of 400-700 nm called?
Visible light
What is the preferred range of colors (wavelength) for an apple, causing it to look red to our eyes?
490-500 nm (blue and green)
What are the main components of a spectrophotometer used to make absorbance or transmittance measurements?
Lamp, monochromator, detector, amplifier
What are the three different types of cuvettes commonly used in spectroscopic experiments, along with their usable wavelengths?
Glass (380-780 nm), Plastic (380-780 nm), Quartz (below 380 nm)
What is the recommended precaution for cleaning absorption cells (cuvets) before filling the sample for measurement?
Always clean the cells thoroughly and rinse at least once with a portion of the sample.
What is the recommended action immediately after using absorption cells (cuvets) for measurement?
Clean cells thoroughly immediately after use.
What is the precaution for cleaning the exposed surface of the cells after filling the sample for measurement?
Always wipe the exposed surface of the cells dry and free from finger prints, using tissue paper.
What is the recommended duration for leaving solutions in the cells after measurement?
Do not leave solutions, particularly strong alkali, in the cells for periods more than an hour.
What should not be used for cleaning absorption cells (cuvets) as it may scratch the optical surfaces?
Never use a brush or any tool for cleaning which may scratch the optical surfaces.
This quiz provides an introduction to the first lab session of the Clinical Biochemistry module at Cihan University in Sulaimaniya. It covers the overview of medical and clinical biochemistry and the practical subjects for the first week.
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