Cihan University Clinical Biochemistry Lab 1 Introduction

Medical Biochemistry deals with the chemical basis of the human body, while Clinical Biochemistry deals with clinical diseases/pathological conditions of the human body.

The practical module will cover subjects such as spectrophotometry, determination of serum glucose, serum urea, serum creatinine, serum bilirubin, AST and ALT, serum TG, serum cholesterol, ALP, CPK, amylase, and LDL/HDL cholesterol test.

The purpose of spectrophotometry is to determine the Lambda Max of substances and to measure the concentration of substances in solution using light.

<p>The fundamental principle of photometric measurement is the use of light for the measurement of the concentration of substances in solution.</p> Signup and view all the answers

<p>In Practical Week #3, the determination of Serum Glucose will be covered.</p> Signup and view all the answers

<p>The subject of focus in Practical Week #12 will be the determination of amylase.</p> Signup and view all the answers

<p>Absorption of light</p> Signup and view all the answers

<p>Visible light</p> Signup and view all the answers

<p>490-500 nm (blue and green)</p> Signup and view all the answers

<p>Lamp, monochromator, detector, amplifier</p> Signup and view all the answers

<p>Glass (380-780 nm), Plastic (380-780 nm), Quartz (below 380 nm)</p> Signup and view all the answers

<p>Always clean the cells thoroughly and rinse at least once with a portion of the sample.</p> Signup and view all the answers

<p>Clean cells thoroughly immediately after use.</p> Signup and view all the answers

<p>Always wipe the exposed surface of the cells dry and free from finger prints, using tissue paper.</p> Signup and view all the answers

<p>Do not leave solutions, particularly strong alkali, in the cells for periods more than an hour.</p> Signup and view all the answers

<p>Never use a brush or any tool for cleaning which may scratch the optical surfaces.</p> Signup and view all the answers