Podcast
Questions and Answers
What specific temperature and pressure conditions are required for sterilizing Blood Agar?
What specific temperature and pressure conditions are required for sterilizing Blood Agar?
Blood Agar must be sterilized at 15 lbs pressure and 121°C.
Why is mammalian blood added to the medium at a temperature of 80-85°C?
Why is mammalian blood added to the medium at a temperature of 80-85°C?
Mammalian blood is added at this temperature to prevent destruction of the blood cells while ensuring they have been lysed.
What is the primary use of Chocolate Agar in microbiology?
What is the primary use of Chocolate Agar in microbiology?
Chocolate Agar is primarily used for the isolation and cultivation of fastidious microorganisms, such as Haemophilus influenzae.
What distinguishes Chocolate Agar from regular Blood Agar?
What distinguishes Chocolate Agar from regular Blood Agar?
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Name two control organisms that are typically tested on Chocolate Agar and their significance.
Name two control organisms that are typically tested on Chocolate Agar and their significance.
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What is the main purpose of adding heated mammalian blood to Chocolate Agar?
What is the main purpose of adding heated mammalian blood to Chocolate Agar?
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At what temperature should the mammalian blood be added to Chocolate Agar during preparation?
At what temperature should the mammalian blood be added to Chocolate Agar during preparation?
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Why is Chocolate Agar named so if it does not contain chocolate?
Why is Chocolate Agar named so if it does not contain chocolate?
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What is one characteristic of the organisms that can be cultivated on Chocolate Agar?
What is one characteristic of the organisms that can be cultivated on Chocolate Agar?
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What is the initial step in preparing Chocolate Agar before adding blood?
What is the initial step in preparing Chocolate Agar before adding blood?
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Chocolate Agar contains heated mammalian ______, typically at a concentration of 5-10%.
Chocolate Agar contains heated mammalian ______, typically at a concentration of 5-10%.
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The procedure for making Chocolate Agar includes autoclaving at 15lbs pressure, 121°C for ______ minutes.
The procedure for making Chocolate Agar includes autoclaving at 15lbs pressure, 121°C for ______ minutes.
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After adding blood at 80-85°C, the mixture is cooled to ______ °C before pouring into sterile Petri plates.
After adding blood at 80-85°C, the mixture is cooled to ______ °C before pouring into sterile Petri plates.
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Chocolate Agar is used for the isolation and cultivation of fastidious microorganisms, such as ______ influenzae.
Chocolate Agar is used for the isolation and cultivation of fastidious microorganisms, such as ______ influenzae.
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No chocolate is actually contained in the plate; it is named for its ______.
No chocolate is actually contained in the plate; it is named for its ______.
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Study Notes
Chocolate Agar Composition
- Contains heated mammalian blood (typically sheep or horse), usually 5-10%.
Preparation Procedure
- Dissolve 40g of blood agar in 1000ml distilled water by heating to boiling.
- Sterilize by autoclaving at 15lbs pressure, 121°C for 15 minutes.
- Add 5-10% mammalian blood at 80-85°C (blood cells lysed at 56°C).
- Cool the mixture to 45-50°C and pour into sterile Petri dishes.
Purpose
- Used to isolate and cultivate a range of fastidious microorganisms, including respiratory bacteria like Haemophilus influenzae.
Naming
- Named for its brown or "chocolate" color, no actual chocolate is present.
Control Organisms
- Haemophilus influenzae and Neisseria gonorrhoeae show good growth on chocolate agar.
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Description
This quiz covers the composition and preparation of chocolate agar, a specialized medium used for isolating fastidious microorganisms. Learn about the procedure and purpose behind its use, as well as the control organisms commonly tested on this agar. Enhance your understanding of this critical laboratory process.
