Charge Distribution in Organic Molecules
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Questions and Answers

Which group always carries a positive charge in an amino acid?

  • Carboxyl group
  • Rest group
  • Amino group (correct)
  • Alpha carbon
  • What determines the type and nature of an amino acid?

  • The amino group
  • The alpha carbon
  • The carboxyl group
  • The side chains (correct)
  • Which atom carries a proton in an amino acid?

  • Carboxyl group
  • Alpha carbon (correct)
  • Rest group
  • Amino group
  • What is the basic structure of an amino acid?

    <p>Alpha carbon and rest group</p> Signup and view all the answers

    Which group is charged in an amino acid?

    <p>Rest group</p> Signup and view all the answers

    What can vary in an amino acid?

    <p>The side chains</p> Signup and view all the answers

    What determines the nature of a protein?

    <p>The side chains</p> Signup and view all the answers

    Which amino acid is made up of a simple side chain?

    <p>Glycin</p> Signup and view all the answers

    What is the central atom in an amino acid called?

    <p>Alpha carbon</p> Signup and view all the answers

    What does gluing amino acids together form?

    <p>Proteins</p> Signup and view all the answers

    Which part of an amino acid determines its identity?

    <p>The side chain</p> Signup and view all the answers

    What determines whether an amino acid is charged or non-charged?

    <p>The side chain</p> Signup and view all the answers

    Which type of amino acid is not fond of water?

    <p>Non-charged apolar</p> Signup and view all the answers

    Which type of amino acid likes water but is still non-charged?

    <p>Polar</p> Signup and view all the answers

    Which type of amino acid has a negative charge in its side chain?

    <p>Acid-charged</p> Signup and view all the answers

    Which type of amino acid is a zwitter ion?

    <p>Basic</p> Signup and view all the answers

    What does the term 'zwitter' mean in German?

    <p>Both</p> Signup and view all the answers

    What happens to the charges of the carboxyl and amino groups in an amino acid?

    <p>Carboxyl becomes positive, amino becomes negative</p> Signup and view all the answers

    Do non-ionized amino acids exist?

    <p>No</p> Signup and view all the answers

    What are the two major characteristics of amino acids?

    <p>Side chain and zwitter ions</p> Signup and view all the answers

    Which part of an amino acid determines its charge in different environments?

    <p>The side chain</p> Signup and view all the answers

    What happens to the charge of an amino acid in an acidic environment?

    <p>The charge becomes positive</p> Signup and view all the answers

    What happens to the charge of an amino acid in a basic environment?

    <p>The charge becomes negative</p> Signup and view all the answers

    What is the net charge of an amino acid in a basic environment?

    <p>Negative</p> Signup and view all the answers

    What is the net charge of an amino acid in an acidic environment?

    <p>Positive</p> Signup and view all the answers

    What happens to the charge of the carboxyl group in an amino acid in an acidic environment?

    <p>The charge becomes negative</p> Signup and view all the answers

    What happens to the charge of the amino group in an amino acid in an acidic environment?

    <p>The charge becomes positive</p> Signup and view all the answers

    What happens to the charge of the carboxyl group in an amino acid in a basic environment?

    <p>The charge becomes positive</p> Signup and view all the answers

    What happens to the charge of the amino group in an amino acid in a basic environment?

    <p>The charge becomes negative</p> Signup and view all the answers

    What can lead to denaturation of a protein?

    <p>Adding a huge amount of acid</p> Signup and view all the answers

    Which type of environment is created in a protein when a lot of minor acids are added together?

    <p>Polar environment</p> Signup and view all the answers

    In which domain of a protein is it advantageous to have a polar region?

    <p>Polar domain</p> Signup and view all the answers

    What is the term used to describe the ability of an amino acid to dissolve in water?

    <p>Hydrophilicity</p> Signup and view all the answers

    Is the apolarity of an amino acid stronger or weaker than its ability to dissolve in water?

    <p>Weaker</p> Signup and view all the answers

    What is the term used to describe an amino acid with a non-polar side chain?

    <p>Non-polar amino acid</p> Signup and view all the answers

    Which type of amino acid is less fond of water?

    <p>Non-polar amino acid</p> Signup and view all the answers

    Which type of amino acid carries a positive charge in its side chain?

    <p>Charged amino acid</p> Signup and view all the answers

    What happens to the charges of the carboxyl and amino groups in an amino acid?

    <p>They become neutral</p> Signup and view all the answers

    What is the term used to describe an amino acid that has both positive and negative charges?

    <p>Zwitterion</p> Signup and view all the answers

    What type of environment does a protein with a polar domain interact with?

    <p>Lipid environment</p> Signup and view all the answers

    Which of the following best describes the isoelectric point (PI) of a molecule in a solution?

    <p>The pH at which a molecule has no net charge</p> Signup and view all the answers

    What does the term 'zwitter ions' refer to in the context of amino acids?

    <p>Amino acids with an equal amount of positive and negative charges</p> Signup and view all the answers

    Which of the following pH values is most likely to result in an equal amount of positive and negative charges for an amino acid with an acidic side chain?

    <p>pH 2.77</p> Signup and view all the answers

    Which of the following pH values is most likely to result in an equal amount of positive and negative charges for an amino acid with a basic side chain?

    <p>pH 10.0</p> Signup and view all the answers

    What is the main factor that determines whether an amino acid is charged or non-charged?

    <p>The pH of the solution</p> Signup and view all the answers

    Which of the following best describes the solubility of polar amino acids in water?

    <p>Polar amino acids are highly soluble in water</p> Signup and view all the answers

    What does the term 'isoelectric point' refer to in the context of proteins?

    <p>The pH at which a protein has no net charge</p> Signup and view all the answers

    Which of the following best describes the distribution of charges in an amino acid at its isoelectric point?

    <p>An equal amount of positive and negative charges</p> Signup and view all the answers

    Which of the following best describes the nature of a zwitter ion?

    <p>A zwitter ion is a molecule with an equal amount of positive and negative charges</p> Signup and view all the answers

    Which of the following pH values is most likely to result in a net positive charge for an amino acid with a basic side chain?

    <p>pH 10.0</p> Signup and view all the answers

    Which process involves reading the genetic code by nuclear ribosomes?

    <p>Transcription</p> Signup and view all the answers

    Which organelle is responsible for reading the genetic code in translation?

    <p>Ribosomes</p> Signup and view all the answers

    Which of the following is NOT involved in translation?

    <p>Polymerases</p> Signup and view all the answers

    What is the function of ribosomes in translation?

    <p>To read the genetic code</p> Signup and view all the answers

    Which term describes the process of rewriting DNA into messenger RNA?

    <p>Transcription</p> Signup and view all the answers

    What is the name for the process of translating messenger RNA into proteins?

    <p>Translation</p> Signup and view all the answers

    In prokaryotes, how are multiple genes translated at once?

    <p>Polycystronic</p> Signup and view all the answers

    In eukaryotes, how are genes translated?

    <p>Monocystronic</p> Signup and view all the answers

    Where does translation occur in prokaryotes?

    <p>Cytosol</p> Signup and view all the answers

    Where does translation occur in eukaryotes?

    <p>Ribosomes</p> Signup and view all the answers

    Which part of the DNA contains the protein coding sequence?

    <p>Exons</p> Signup and view all the answers

    Where is the genetic code for amino acids located?

    <p>In the nucleus</p> Signup and view all the answers

    Which molecule is responsible for carrying the genetic code from the nucleus to the ribosomes?

    <p>mRNA</p> Signup and view all the answers

    Where is mRNA produced?

    <p>In the nucleus</p> Signup and view all the answers

    What is the process called when the genetic code in mRNA is used to build a protein?

    <p>Translation</p> Signup and view all the answers

    Which part of the cell is responsible for protein synthesis?

    <p>Ribosomes</p> Signup and view all the answers

    What is the product of translation?

    <p>Protein</p> Signup and view all the answers

    Which type of amino acids are important to know for studying proteins?

    <p>Polar and non-polar amino acids</p> Signup and view all the answers

    Do you need to know all the structures and names of amino acids?

    <p>No, it is not necessary at this moment</p> Signup and view all the answers

    What is the role of axons in protein synthesis?

    <p>They lead to the production of proteins</p> Signup and view all the answers

    Which of the following is a characteristic of eukaryotic translation?

    <p>Transport of mRNA out of the nucleus before translation</p> Signup and view all the answers

    What is found at the 5'-end of the messenger RNA in eukaryotes?

    <p>Cap binding complexes</p> Signup and view all the answers

    What surrounds the messenger RNA in eukaryotes?

    <p>Proteins</p> Signup and view all the answers

    What is the function of cap binding complexes (CBC) in eukaryotic translation?

    <p>Binding to the 5'-end cap of the mRNA</p> Signup and view all the answers

    Which of the following is NOT mentioned as a component involved in eukaryotic translation?

    <p>Prokaryotes</p> Signup and view all the answers

    What is the process called when messenger RNA is transported out of the nucleus in eukaryotes?

    <p>Export</p> Signup and view all the answers

    What is the term used to describe the structure at the 5'-end of the messenger RNA in eukaryotes?

    <p>Cap</p> Signup and view all the answers

    What is the role of poly-A binding proteins in eukaryotic translation?

    <p>Binding to the poly-A tail of the mRNA</p> Signup and view all the answers

    What is the function of axon junction complexes in eukaryotic translation?

    <p>Formation of a complex at the axon junction</p> Signup and view all the answers

    What is the function of SR proteins in eukaryotic translation?

    <p>Regulation of alternative splicing</p> Signup and view all the answers

    Which molecule plays a key role in connecting messenger RNA and protein?

    <p>Transfer RNA</p> Signup and view all the answers

    What shape does tRNA resemble?

    <p>Clover leaf</p> Signup and view all the answers

    What is the function of the anti-codon in tRNA?

    <p>Binds to the amino acid</p> Signup and view all the answers

    Where is the amino acid attached in tRNA?

    <p>Three-accent side</p> Signup and view all the answers

    In which direction does the tRNA sequence start?

    <p>Clockwise</p> Signup and view all the answers

    What is the main role of tRNA in protein synthesis?

    <p>Carrying amino acids</p> Signup and view all the answers

    What is the shape of tRNA that is mentioned in the text?

    <p>Clover leaf</p> Signup and view all the answers

    What is the function of tRNA in the process of translation?

    <p>Carrying amino acids</p> Signup and view all the answers

    Which side of tRNA is the amino acid attached to?

    <p>Three-accent side</p> Signup and view all the answers

    What is the shape of tRNA described in the text?

    <p>Clover leaf</p> Signup and view all the answers

    Which of the following is true about the genetic code?

    <p>It is universal</p> Signup and view all the answers

    What is the function of the start codon in the genetic code?

    <p>It codes for methionine</p> Signup and view all the answers

    How many stop codons are there in the genetic code?

    <p>Three</p> Signup and view all the answers

    What is the term used to describe the reading of the genetic code like a zipper?

    <p>Non-overlapping</p> Signup and view all the answers

    How many different amino acids are synthesized in our body?

    <p>20</p> Signup and view all the answers

    What is the term used to describe the fact that several different triplets can code for a single amino acid?

    <p>Degenerative</p> Signup and view all the answers

    What is the function of stop codons in the genetic code?

    <p>They code for the stop codon</p> Signup and view all the answers

    What is the role of ribosomes in the genetic code?

    <p>They facilitate protein synthesis</p> Signup and view all the answers

    What is the term used to describe the fact that the genetic code is read as triplets?

    <p>Triplet code</p> Signup and view all the answers

    What is the term used to describe the fact that the genetic code is read consecutively, without skipping any nucleotides?

    <p>Universal</p> Signup and view all the answers

    Which protein complexes are involved in splicing?

    <p>AJC protein complexes</p> Signup and view all the answers

    What is the role of the poly-A tail and five-accent cap in mRNA?

    <p>Stabilizing mRNA</p> Signup and view all the answers

    What happens when the nuclear export receptor interacts with the nuclear pore?

    <p>The mRNA is transported out of the nucleus</p> Signup and view all the answers

    Which initiation factors bind to the five-accent cap?

    <p>Initiation factors 4G and 4E</p> Signup and view all the answers

    What connects the three-accent tail with the five-accent cap in eukaryotes?

    <p>Poly-A tail</p> Signup and view all the answers

    When does nonsense messenger RNA decay usually occur?

    <p>Before translation initiation</p> Signup and view all the answers

    Which type of genes can code for multiple proteins in one mRNA molecule?

    <p>Prokaryotic genes</p> Signup and view all the answers

    What is the term used to describe the coding and non-coding sequences in mRNA?

    <p>Coding sequence and non-coding sequence</p> Signup and view all the answers

    Where does translation occur in eukaryotes?

    <p>Cytoplasm</p> Signup and view all the answers

    What is the difference between polycistronic and monocistronic mRNA?

    <p>Polycistronic mRNA has multiple coding sequences, while monocistronic mRNA has one coding sequence</p> Signup and view all the answers

    Which subunit is present in prokaryotic ribosomes during translation?

    <p>30S</p> Signup and view all the answers

    Which subunit is present in eukaryotic ribosomes during translation?

    <p>60S</p> Signup and view all the answers

    What type of RNA is required for translation?

    <p>mRNA</p> Signup and view all the answers

    What is the role of elongation factors in translation?

    <p>Facilitating the movement of ribosomes along mRNA</p> Signup and view all the answers

    What is the function of initiation factors in translation?

    <p>Initiation of translation</p> Signup and view all the answers

    What molecule is responsible for carrying the amino acid during translation?

    <p>tRNA</p> Signup and view all the answers

    Which molecule is responsible for attaching the amino acid to the tRNA?

    <p>tRNA synthetase</p> Signup and view all the answers

    What molecule provides the energy for translation?

    <p>GTP</p> Signup and view all the answers

    Which process is different in prokaryotic translation compared to eukaryotic translation?

    <p>Initiation</p> Signup and view all the answers

    What is the function of the ribosomal subunits in translation?

    <p>Initiation of translation</p> Signup and view all the answers

    Which of the following is the correct number of tRNAs required for the 61 different codons for amino acids?

    <p>20</p> Signup and view all the answers

    What is the term used to describe the process of tRNA coupling with amino acids?

    <p>Bubble binding</p> Signup and view all the answers

    Which nucleotide in the triplet at the triaxon side allows for the interaction between a G and a U in the tRNA recognition process?

    <p>G</p> Signup and view all the answers

    What is the name of the enzymatic complex responsible for coupling amino acids to tRNA molecules?

    <p>Amino-acetyl transphorases</p> Signup and view all the answers

    How many different amino-acetyl transphorases are there, corresponding to the 20 different amino acids?

    <p>20</p> Signup and view all the answers

    Which molecule is required for coupling valine to a specific tRNA molecule?

    <p>ATP</p> Signup and view all the answers

    What is the term used to describe the process of coupling amino acids to tRNA molecules?

    <p>Amino-acetyl coupling</p> Signup and view all the answers

    What is the specific function of amino-acetyl transphorases?

    <p>Coupling amino acids to tRNA molecules</p> Signup and view all the answers

    Which amino acid is used as an example to explain the coupling process with tRNA molecules?

    <p>Valine</p> Signup and view all the answers

    What is the name of the tRNA molecule that specifically recognizes valine?

    <p>tRNA-Valine</p> Signup and view all the answers

    What is the role of the wobble effect in translation?

    <p>It allows for recognition of different codons by the same tRNA molecule.</p> Signup and view all the answers

    What is the function of an aminoacyl-tRNA synthetase?

    <p>It attaches an amino acid to its specific tRNA molecule.</p> Signup and view all the answers

    What is the significance of an inosine nucleoside in the anticodon of tRNA?

    <p>It allows for recognition of multiple codons by the same tRNA molecule.</p> Signup and view all the answers

    What is the role of the aminoacyl-tRNA synthetase complex in translation?

    <p>It attaches an amino acid to its specific tRNA molecule.</p> Signup and view all the answers

    What is the significance of the CCA sequence at the 3' end of tRNA?

    <p>It serves as the site for amino acid attachment.</p> Signup and view all the answers

    What is the function of the aminoacyl-tRNA synthetase enzyme?

    <p>It attaches an amino acid to its specific tRNA molecule.</p> Signup and view all the answers

    What is the role of the wobble pairing in translation?

    <p>It allows for recognition of different codons by the same tRNA molecule.</p> Signup and view all the answers

    What is the significance of the inosine nucleoside in the anticodon of tRNA?

    <p>It allows for recognition of multiple codons by the same tRNA molecule.</p> Signup and view all the answers

    What is the function of the aminoacyl-tRNA synthetase complex in translation?

    <p>It attaches an amino acid to its specific tRNA molecule.</p> Signup and view all the answers

    What is the significance of the CCA sequence at the 3' end of tRNA?

    <p>It serves as the site for amino acid attachment.</p> Signup and view all the answers

    Which of the following is the correct order of the three phases in the translation process?

    <p>Initiation, termination, elongation</p> Signup and view all the answers

    What is the name of the sequence that is recognized by the small ribosomal complex in prokaryotic translation?

    <p>Shine-Dalgano sequence</p> Signup and view all the answers

    Which component of the ribosome recognizes the Shine-Dalgano sequence in the messenger RNA?

    <p>16S ribosomal RNA</p> Signup and view all the answers

    What are the three different phases of the translation process?

    <p>Initiation, elongation, termination</p> Signup and view all the answers

    What is the composition of ribosomes?

    <p>Ribosomal RNA and proteins</p> Signup and view all the answers

    Which ribosomal complex recognizes the Shine-Dalgano sequence in prokaryotic translation?

    <p>30S</p> Signup and view all the answers

    What is the name of the sequence that has been identified by Mr. Shine and Mr. Dalgano?

    <p>Shine-Dalgano sequence</p> Signup and view all the answers

    What is the role of the 16S ribosomal RNA in prokaryotic translation?

    <p>It recognizes the Shine-Dalgano sequence</p> Signup and view all the answers

    What is the name of the small ribosomal complex in prokaryotes?

    <p>30S</p> Signup and view all the answers

    What are the players involved in the translation process?

    <p>Release factors and ribosomal recycling factors</p> Signup and view all the answers

    Which of the following is true about the 70S initiation complex?

    <p>It is the complete ribosomal complex consisting of the large and small subunits</p> Signup and view all the answers

    What is the role of initiation factors in translation?

    <p>To bind the ribosomal complex to the mRNA molecule</p> Signup and view all the answers

    What is the function of the A site in the ribosomal complex?

    <p>Arrival</p> Signup and view all the answers

    What is the specific feature of methionine in prokaryotes?

    <p>It is a bit changed with a formula group attached</p> Signup and view all the answers

    Why are the ribosomal complexes specifically called 30S and 50S?

    <p>Based on the size determined by the Svetberg coefficient</p> Signup and view all the answers

    What happens to GTP in the formation of the 70S initiation complex?

    <p>It is hydrolyzed into GDP</p> Signup and view all the answers

    What is the role of the P site in the ribosomal complex?

    <p>Protein or peptide</p> Signup and view all the answers

    What is the role of the E site in the ribosomal complex?

    <p>Exit</p> Signup and view all the answers

    What is the function of the Shine-Dalgano sequence in translation?

    <p>To bind the ribosomal complex to the mRNA molecule</p> Signup and view all the answers

    Where does the methionine bind in prokaryotes?

    <p>Before the 30S ribosomal complex</p> Signup and view all the answers

    Which of the following is responsible for the enzymatic activity of gluing amino acids together in the ribosomal complex?

    <p>Ribosomal RNA</p> Signup and view all the answers

    What is the name of the enzyme that performs the gluing of amino acids together in the ribosomal complex?

    <p>Peptidyl transferase</p> Signup and view all the answers

    What is the function of the A site in the ribosomal complex during translation?

    <p>To hold the tRNA containing proline</p> Signup and view all the answers

    What is the function of the P site in the ribosomal complex during translation?

    <p>To hold the amino acids</p> Signup and view all the answers

    What is the function of the E site in the ribosomal complex during translation?

    <p>To hold the mRNA</p> Signup and view all the answers

    What is the name of the specific tRNA that recognizes and binds to the amino acid proline?

    <p>Proline</p> Signup and view all the answers

    What is the term used to describe the bond formed between two amino acids in the ribosomal complex?

    <p>Peptide bond</p> Signup and view all the answers

    What is the role of the enzyme called aminoacyl-tRNA synthetase?

    <p>To recognize and bind specific amino acids to tRNA molecules</p> Signup and view all the answers

    What is the term used to describe the site in the ribosomal complex where translation occurs?

    <p>Ribosome</p> Signup and view all the answers

    What is the term used to describe the process of adding amino acids to the growing polypeptide chain during translation?

    <p>Translation</p> Signup and view all the answers

    Which molecule is responsible for hydrolyzing GDP into GTP, providing the energy for translocation during translation?

    <p>Elongation factor g</p> Signup and view all the answers

    What is the role of the ribosomal recycling factor in translation?

    <p>To release the release factor one</p> Signup and view all the answers

    Which of the following is NOT a step in the initiation phase of translation?

    <p>Binding of the 50S ribosomal subunit to the mRNA</p> Signup and view all the answers

    What happens to the uncharged initiation tRNA during translation?

    <p>It is released from the ribosomal complex</p> Signup and view all the answers

    Which molecule is responsible for recognizing and binding to the stop codon during translation termination?

    <p>Release factor one</p> Signup and view all the answers

    During translation, what is the function of the peptidyl RNA?

    <p>It catalyzes the formation of peptide bonds.</p> Signup and view all the answers

    What is the role of the peptidyl transferase enzyme during translation?

    <p>To form a peptide bond</p> Signup and view all the answers

    What happens during the translocation phase of translation?

    <p>The ribosomal complex shifts three positions.</p> Signup and view all the answers

    What is the role of the P side in the ribosomal complex?

    <p>It attaches the amino acids to the growing polypeptide chain.</p> Signup and view all the answers

    Which of the following is NOT a component of the ribosomal complex during translation?

    <p>Elongation factor g</p> Signup and view all the answers

    What is the function of the A site in the ribosomal complex during translation?

    <p>To bind the second tRNA to the codon</p> Signup and view all the answers

    What happens to the empty tRNA during translation?

    <p>It is released from the ribosomal complex.</p> Signup and view all the answers

    What is the name of the process that adds amino acids to the growing polypeptide chain?

    <p>Elongation</p> Signup and view all the answers

    Which molecule provides the energy for hydrolyzing the GDP into GTP during translation?

    <p>Elongation factor g</p> Signup and view all the answers

    What is the role of the release factor in translation termination?

    <p>To recognize and bind to the stop codon</p> Signup and view all the answers

    What is the role of the stop codon in translation?

    <p>It signals the termination of translation.</p> Signup and view all the answers

    What is the name of the phase where the translation process terminates?

    <p>Termination</p> Signup and view all the answers

    What is the function of the stop codon in translation?

    <p>It signals the termination of translation.</p> Signup and view all the answers

    What happens to the polypeptide chain once it is fully synthesized?

    <p>It is released from the ribosomal complex.</p> Signup and view all the answers

    What is the name of the process that adds amino acids to the growing polypeptide chain?

    <p>Elongation</p> Signup and view all the answers

    Which site in the ribosome is responsible for the release of the uncharged tRNA molecule?

    <p>E-site</p> Signup and view all the answers

    What usually happens to the messenger RNA (mRNA) molecule after translation is completed?

    <p>It is degraded</p> Signup and view all the answers

    What causes the tRNA molecule to leave the ribosome?

    <p>Electrostatic forces</p> Signup and view all the answers

    What happens to the tRNA molecules after they leave the ribosome?

    <p>They are reused</p> Signup and view all the answers

    What is the name of the bond between tRNA and an amino acid?

    <p>Peptide bond</p> Signup and view all the answers

    What does the 'A' in the 'A-site' of the ribosome stand for?

    <p>Arrival</p> Signup and view all the answers

    Are all proteins terminated with a methionine amino acid?

    <p>No</p> Signup and view all the answers

    What is the correct sequence for the codon that codes for the amino acid proline?

    <p>CCG</p> Signup and view all the answers

    Is pyridil transferase a protein?

    <p>No</p> Signup and view all the answers

    What is the name of the bond between tRNA and a minor acid?

    <p>Ester bond</p> Signup and view all the answers

    During translation, why does the start code for the minus it, but the stop codes don't?

    <p>The start code is needed to glue on the first minus it</p> Signup and view all the answers

    How does the ribosomal complex move along the M-meshny RNA during elongation?

    <p>The ribosomal complex moves along the M-meshny RNA</p> Signup and view all the answers

    How is the T-RNA being removed during translation?

    <p>It is released from the ribosome through conformational changes</p> Signup and view all the answers

    What happens to a T-RNA that is not charged anymore during translation?

    <p>It remains in the A-site of the ribosome</p> Signup and view all the answers

    What is the current understanding of the movement of the M-meshny RNA during translation?

    <p>It moves along with the ribosomal complex</p> Signup and view all the answers

    What is the role of the start code in translation?

    <p>To provide a site for the first minus it to bind</p> Signup and view all the answers

    What is the role of the stop codes in translation?

    <p>To indicate the completion of translation</p> Signup and view all the answers

    What is the current understanding of the movement of the ribosomal complex along the M-meshny RNA during elongation?

    <p>The ribosomal complex moves along the M-meshny RNA</p> Signup and view all the answers

    How does the ribosomal complex move along the M-meshny RNA during translation?

    <p>The ribosomal complex moves along the M-meshny RNA</p> Signup and view all the answers

    What happens to the T-RNA during translation?

    <p>It is released from the ribosome through conformational changes</p> Signup and view all the answers

    Which of the following is a difference between eukaryotic and prokaryotic translation?

    <p>Eukaryotic translation does not have a Shine-Dalgano sequence.</p> Signup and view all the answers

    What is the role of the Kozak sequence in eukaryotic translation?

    <p>It contains the AUG start codon.</p> Signup and view all the answers

    What is the function of the initiation factors in eukaryotic translation?

    <p>They bind to the 5'-accent cap.</p> Signup and view all the answers

    What is the composition of the poly-A tail in eukaryotic translation?

    <p>It is surrounded by poly-A binding protein complexes.</p> Signup and view all the answers

    What happens when the initiation factors are bound to the 5'-accent cap in eukaryotic translation?

    <p>They form a loop with the poly-A tail.</p> Signup and view all the answers

    What is the role of the poly-A binding protein complexes in eukaryotic translation?

    <p>They form a loop with the poly-A tail.</p> Signup and view all the answers

    What is the function of the 5'-accent cap in eukaryotic translation?

    <p>It binds to the initiation factors.</p> Signup and view all the answers

    What is the purpose of the looping between the 5'-accent cap and the poly-A tail in eukaryotic translation?

    <p>To facilitate translation initiation.</p> Signup and view all the answers

    What is the role of the AUG start codon in eukaryotic translation?

    <p>It codes for the first amino acid.</p> Signup and view all the answers

    What is the difference between eukaryotic and prokaryotic mRNA molecules?

    <p>Eukaryotic mRNA contains a 5'-accent cap and poly-A tail.</p> Signup and view all the answers

    What is the current understanding about the movement of the ribosomal complex along the mRNA during translation?

    <p>The ribosomal complex moves along the mRNA molecule</p> Signup and view all the answers

    Why does the start codon have a different function compared to the stop codons during translation?

    <p>The start codon is necessary to attach the first amino acid to the growing protein chain</p> Signup and view all the answers

    How is an uncharged tRNA molecule removed from the ribosome during translation?

    <p>It is released from the ribosome and exits the translation complex</p> Signup and view all the answers

    What determines the movement of the ribosome along the mRNA during translation?

    <p>The translocation of the ribosomal complex along the mRNA</p> Signup and view all the answers

    What is the role of the E-site in the ribosome during translation?

    <p>It is responsible for the release of the uncharged tRNA molecule</p> Signup and view all the answers

    What is the current understanding about the movement of the ribosomal complex along the mRNA during translation?

    <p>The ribosomal complex moves along the mRNA molecule</p> Signup and view all the answers

    What is the role of the start codon in translation?

    <p>To attach the first amino acid to the growing protein chain</p> Signup and view all the answers

    What is the role of the stop codons in translation?

    <p>To signal the ribosome to release the completed protein</p> Signup and view all the answers

    What is the current understanding about the movement of the ribosomal complex along the mRNA during translation?

    <p>The ribosomal complex moves along the mRNA molecule</p> Signup and view all the answers

    How is an uncharged tRNA molecule removed from the ribosome during translation?

    <p>It is released from the ribosome and exits the translation complex</p> Signup and view all the answers

    Which ribosomal subunit is present in eukaryotes during translation?

    <p>40S</p> Signup and view all the answers

    What is the role of the initiation tRNA molecule in eukaryotic translation?

    <p>To interact with the AUG start codon</p> Signup and view all the answers

    What is the size of the ribosomal complex formed by the small and large ribosomal subunits in eukaryotes?

    <p>80S</p> Signup and view all the answers

    What is the function of the scanning process during translation in eukaryotes?

    <p>To find the AUG start codon</p> Signup and view all the answers

    Which ribosomal subunit is larger in eukaryotes compared to prokaryotes?

    <p>60S</p> Signup and view all the answers

    What is the role of the initiation factors in eukaryotic translation?

    <p>To initiate the scanning process</p> Signup and view all the answers

    What is the size of the small ribosomal subunit in eukaryotes?

    <p>40S</p> Signup and view all the answers

    What is the role of the 5' cap in eukaryotic translation?

    <p>To anchor the 40S ribosomal subunit</p> Signup and view all the answers

    What is the role of the large ribosomal subunit in eukaryotic translation?

    <p>To catalyze peptide bond formation</p> Signup and view all the answers

    What is the size of the large ribosomal subunit in eukaryotes?

    <p>60S</p> Signup and view all the answers

    What is the function of ribosomes in eukaryotes?

    <p>To synthesize all types of proteins</p> Signup and view all the answers

    What is the purpose of polyribosomes in eukaryotes?

    <p>To increase the efficiency of protein synthesis</p> Signup and view all the answers

    What is the significance of leaky scanning in eukaryotes?

    <p>It allows for the synthesis of multiple proteins from one gene</p> Signup and view all the answers

    What is the difference between ribosomes in prokaryotes and eukaryotes?

    <p>Eukaryotic ribosomes are floating in the cytosol</p> Signup and view all the answers

    What type of proteins do ribosomes attached to the endoplasmic reticulum synthesize?

    <p>Membrane attached proteins</p> Signup and view all the answers

    What is the purpose of simultaneous translation in eukaryotes?

    <p>To increase the efficiency of protein synthesis</p> Signup and view all the answers

    What is the role of the endoplasmic reticulum in protein synthesis?

    <p>To synthesize all types of proteins</p> Signup and view all the answers

    What does leaky scanning refer to in eukaryotic translation?

    <p>The synthesis of multiple proteins from one gene</p> Signup and view all the answers

    What is the purpose of monogestronic translation in eukaryotes?

    <p>To synthesize one gene at a time</p> Signup and view all the answers

    What is the function of ribosomes in prokaryotes?

    <p>To synthesize cytoplasmic proteins</p> Signup and view all the answers

    Which complex is responsible for scanning the mRNA for the start codon in eukaryotes?

    <p>40S ribosome complex</p> Signup and view all the answers

    What is the term used to describe the process of translating an mRNA molecule with a premature stop codon?

    <p>Nonsense mediated mRNA decay</p> Signup and view all the answers

    Why does nonsense mediated mRNA decay exist?

    <p>To prevent translation of abnormal proteins</p> Signup and view all the answers

    What happens if an mRNA molecule undergoes abnormal splicing and contains a premature stop codon?

    <p>The mRNA is degraded</p> Signup and view all the answers

    What is the role of the splicing complexes in eukaryotes?

    <p>To remove introns from mRNA</p> Signup and view all the answers

    What is the consequence of abnormal splicing in mRNA molecules?

    <p>The production of incorrect proteins</p> Signup and view all the answers

    What is the function of the start codon in the genetic code?

    <p>To initiate translation</p> Signup and view all the answers

    What is the consequence of a premature stop codon in an mRNA molecule?

    <p>The production of a truncated protein</p> Signup and view all the answers

    What is the term used to describe the process of removing introns from mRNA?

    <p>Splicing</p> Signup and view all the answers

    Why is it important to prevent the translation of abnormal proteins?

    <p>Abnormal proteins can disrupt cellular processes</p> Signup and view all the answers

    What is the function of UPF proteins in translation?

    <p>They recognize premature stop codons and degrade the Mestinia RNA.</p> Signup and view all the answers

    How do microRNAs regulate translation in eukaryotes?

    <p>They can interfere with the looping of messenger RNA, inhibiting translation initiation.</p> Signup and view all the answers

    What is the purpose of sequestration bodies in the cell?

    <p>To store messenger RNA molecules for rapid translation.</p> Signup and view all the answers

    What is the role of the poly-A tail on messenger RNA?

    <p>It enhances the binding of ribosomal complexes to messenger RNA.</p> Signup and view all the answers

    How do microRNAs inhibit the ribosomal complex during translation?

    <p>They bind to the poly-A tail and prevent the looping of messenger RNA.</p> Signup and view all the answers

    What happens when the poly-A tail on messenger RNA is de-adenylated?

    <p>The looping of messenger RNA is disrupted, leading to a halt in translation initiation.</p> Signup and view all the answers

    What is the main function of microRNAs in translation?

    <p>To regulate the translation process by inhibiting initiation or degradation.</p> Signup and view all the answers

    Why is sequestration of messenger RNA molecules important in the cell?

    <p>It allows for rapid translation of stored messenger RNA molecules.</p> Signup and view all the answers

    What is the effect of microRNAs on the translation process?

    <p>They can inhibit the initiation and progress of translation.</p> Signup and view all the answers

    What is the significance of premature stop codon recognition by UPF proteins?

    <p>It prevents the formation of incorrect proteins by degrading the Mestinia RNA.</p> Signup and view all the answers

    Which part of the mRNA molecule is the 5'-cent cap located?

    <p>At the 5'-cent side</p> Signup and view all the answers

    What is the function of the 5'-cent cap in mRNA?

    <p>To protect the mRNA from degradation</p> Signup and view all the answers

    What is the role of the rough ER in protein synthesis?

    <p>To translate mRNA into protein</p> Signup and view all the answers

    What is the difference between translation in prokaryotes and eukaryotes?

    <p>Prokaryotes have ribosomes in the cytoplasm, while eukaryotes have ribosomes on the rough ER</p> Signup and view all the answers

    What will be discussed in the second part of the lecture?

    <p>All of the above</p> Signup and view all the answers

    What is the role of tRNA during translation?

    <p>To carry amino acids to the ribosomes</p> Signup and view all the answers

    What is the function of the 3'-cent poly-A tail in mRNA?

    <p>To protect the mRNA from degradation</p> Signup and view all the answers

    What is the significance of the 5'-cent cap and the 3'-cent poly-A tail in mRNA?

    <p>They protect the mRNA from degradation</p> Signup and view all the answers

    What is the function of the stop codon in translation?

    <p>To signal the end of protein synthesis</p> Signup and view all the answers

    What is the function of the start codon in translation?

    <p>To initiate translation</p> Signup and view all the answers

    What is the approximate number of different proteins that can be produced from the 23,000 protein coding genes in human beings?

    <p>1 million</p> Signup and view all the answers

    What is the role of eukaryotic RNA in the production of different proteins?

    <p>Monitoring protein production</p> Signup and view all the answers

    How can different proteins be produced from a single messenger RNA molecule?

    <p>By scanning the messenger RNA sequence</p> Signup and view all the answers

    How do the functions of proteins depend on their folding?

    <p>Proteins with different folding always have different functions</p> Signup and view all the answers

    What is the purpose of microRNAs in the storage of messenger RNA molecules?

    <p>To bring the messenger RNA to the ribosome complex</p> Signup and view all the answers

    Where does the ribosome first attach during eukaryotic translation?

    <p>To the 5' accent cap</p> Signup and view all the answers

    What is the function of EF4F in translation?

    <p>To anchor the ribosomal subunit</p> Signup and view all the answers

    What is the difference between prokaryotic and eukaryotic translation?

    <p>Eukaryotic mRNA is translated by multiple ribosomes</p> Signup and view all the answers

    How is collision prevented when multiple ribosomes are working on the same RNA?

    <p>The ribosomes move at the same pace</p> Signup and view all the answers

    Is the presence of a stop codon always a mistake in mRNA splicing?

    <p>It depends on the context</p> Signup and view all the answers

    Which process is involved in the regulation of translation by microRNAs?

    <p>Attachment to messenger RNA</p> Signup and view all the answers

    What happens when there is no polyethyl present during translation?

    <p>Translation is stopped</p> Signup and view all the answers

    Where can messenger RNA molecules be stored in the cell?

    <p>Sacrosanation bodies</p> Signup and view all the answers

    What is the term used to describe the regulation of translation by microRNAs?

    <p>Translational regulation</p> Signup and view all the answers

    When does translation stop?

    <p>When there is no polyethyl</p> Signup and view all the answers

    What is the function of microRNAs in translation?

    <p>Regulation of translation</p> Signup and view all the answers

    What is the role of the 5'-end cap in eukaryotic translation?

    <p>Protection of mRNA</p> Signup and view all the answers

    What is the final part of the translation process involving microRNAs?

    <p>Attachment to sacrosanation bodies</p> Signup and view all the answers

    What is the effect of microRNAs on messenger RNA molecules?

    <p>Degradation</p> Signup and view all the answers

    What is the role of polyadenylation in translation?

    <p>None of the above</p> Signup and view all the answers

    Which of the following is a characteristic of prokaryotic transcription?

    <p>Direct translation occurs while transcription is still going on.</p> Signup and view all the answers

    What is the difference between leaky scanning and polycistronic?

    <p>Leaky scanning occurs on one gene, while polycistronic involves different genes.</p> Signup and view all the answers

    What is the function of microRNAs in translation?

    <p>They prevent looping of the mRNA.</p> Signup and view all the answers

    What is the role of ribosomes in prokaryotic translation?

    <p>They are responsible for direct translation.</p> Signup and view all the answers

    What is the consequence of abnormal splicing in mRNA molecules?

    <p>Proteins cannot be properly folded.</p> Signup and view all the answers

    What is the function of the poly-A binding protein complexes in eukaryotic translation?

    <p>They bind to the poly-A tail and enhance translation.</p> Signup and view all the answers

    What is the difference between prokaryotic and eukaryotic translation?

    <p>Prokaryotic translation involves polycistronic genes, while eukaryotic translation involves monocistronic genes.</p> Signup and view all the answers

    What is the function of the start codon in translation?

    <p>It initiates translation.</p> Signup and view all the answers

    What is the function of tRNA in protein synthesis?

    <p>It carries amino acids to the ribosomal complex.</p> Signup and view all the answers

    How are multiple genes translated at once in prokaryotes?

    <p>They are translated in a polycistronic manner.</p> Signup and view all the answers

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