Podcast
Questions and Answers
Which mechanism is responsible for limiting the transcript level by either suppressing transcription or activating a sequence-specific RNA degradation process?
Which mechanism is responsible for limiting the transcript level by either suppressing transcription or activating a sequence-specific RNA degradation process?
Which enzyme cleaves double-stranded siRNA into smaller fragments?
Which enzyme cleaves double-stranded siRNA into smaller fragments?
What is the main function of the RNA-induced silencing complex (RISC)?
What is the main function of the RNA-induced silencing complex (RISC)?
What is the term used to describe a cellular mechanism that uses the gene's own DNA sequence to turn it off?
What is the term used to describe a cellular mechanism that uses the gene's own DNA sequence to turn it off?
Signup and view all the answers
Which type of RNA forms hairpins and is involved in RNA interference (RNAi)?
Which type of RNA forms hairpins and is involved in RNA interference (RNAi)?
Signup and view all the answers
What is the function of loss-of-function mutants in studying gene function?
What is the function of loss-of-function mutants in studying gene function?
Signup and view all the answers
In RNA interference (RNAi), what is often used for silencing or gene knockdown in research?
In RNA interference (RNAi), what is often used for silencing or gene knockdown in research?
Signup and view all the answers
What is triggered by double-stranded RNA (dsRNA) in a wide variety of organisms?
What is triggered by double-stranded RNA (dsRNA) in a wide variety of organisms?
Signup and view all the answers
Which protein binds to the siRNA and discards the sense strand together with other proteins in RNA interference (RNAi)?
Which protein binds to the siRNA and discards the sense strand together with other proteins in RNA interference (RNAi)?
Signup and view all the answers
Which type of RNA is complementary to the sense strand of the mRNA and pairs with the mRNA to either inhibit translation or cleave the mRNA in RNA interference (RNAi)?
Which type of RNA is complementary to the sense strand of the mRNA and pairs with the mRNA to either inhibit translation or cleave the mRNA in RNA interference (RNAi)?
Signup and view all the answers
RNA interference (RNAi) is only triggered by single-stranded RNA (ssRNA).
RNA interference (RNAi) is only triggered by single-stranded RNA (ssRNA).
Signup and view all the answers
RNA silencing can limit the transcript level by suppressing transcription or by activating a sequence-specific RNA degradation process.
RNA silencing can limit the transcript level by suppressing transcription or by activating a sequence-specific RNA degradation process.
Signup and view all the answers
The enzyme Dicer cleaves double-stranded siRNA into smaller fragments in the range of approximately 20 to 22 base pairs.
The enzyme Dicer cleaves double-stranded siRNA into smaller fragments in the range of approximately 20 to 22 base pairs.
Signup and view all the answers
The RISC complex pairs with the mRNA to activate translation and increase protein production.
The RISC complex pairs with the mRNA to activate translation and increase protein production.
Signup and view all the answers
In RNA interference (RNAi), loss-of-function mutants are made in order to study the function of a gene.
In RNA interference (RNAi), loss-of-function mutants are made in order to study the function of a gene.
Signup and view all the answers
In RNA interference (RNAi), short interfering RNA (siRNA) and micro RNA (miRNA) are two types of exogenous dsRNA.
In RNA interference (RNAi), short interfering RNA (siRNA) and micro RNA (miRNA) are two types of exogenous dsRNA.
Signup and view all the answers
The gene's own DNA sequence is used to turn off gene expression in the process of RNA interference (RNAi).
The gene's own DNA sequence is used to turn off gene expression in the process of RNA interference (RNAi).
Signup and view all the answers
In RNA interference (RNAi), the sense strand of the mRNA is complementary to the antisense strand of the siRNA.
In RNA interference (RNAi), the sense strand of the mRNA is complementary to the antisense strand of the siRNA.
Signup and view all the answers
The Argonaut family of proteins is not involved in forming the RNA-induced silencing complex (RISC) in RNA interference (RNAi).
The Argonaut family of proteins is not involved in forming the RNA-induced silencing complex (RISC) in RNA interference (RNAi).
Signup and view all the answers
RNA silencing is not important for development, stress responses, and suppression of viruses, transposons, and transgenes.
RNA silencing is not important for development, stress responses, and suppression of viruses, transposons, and transgenes.
Signup and view all the answers
Match the following components with their roles in the two-component sensor regulator system in bacteria:
Match the following components with their roles in the two-component sensor regulator system in bacteria:
Signup and view all the answers
Match the following terms with their descriptions in gene silencing - RNA interference (RNAi):
Match the following terms with their descriptions in gene silencing - RNA interference (RNAi):
Signup and view all the answers
Match the following components with their roles in the RNA interference (RNAi) process:
Match the following components with their roles in the RNA interference (RNAi) process:
Signup and view all the answers
