Cell Theory & Microscopy Quiz

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Questions and Answers

What is the original size of a fiber if its apparent size under a microscope with 400X magnification is 0.6 mm?

  • 2.4 µm (correct)
  • 0.15 µm
  • 1.5 µm
  • 240 µm

What is the FOV diameter at 400x magnification if the diameter at 40x magnification is 2.0 mm?

  • 2.0 mm
  • 20 mm
  • 0.4 mm
  • 0.2 mm (correct)

What is the estimated size of a specimen that takes up 4 of the field of view at 400x magnification with a FOV diameter of 1.7 mm?

  • 0.425 mm (correct)
  • 1.7 µm
  • 0.425 µm
  • 0.85 mm

What are the three main points of cell theory?

<p>All living things are made of cells, cells are the structural and functional units of life, all cells arise from pre-existing cells (C)</p> Signup and view all the answers

Why do plant cells have chloroplasts while animal cells do not?

<p>Chloroplasts are essential for photosynthesis, which is unique to producers like plants. (C)</p> Signup and view all the answers

What was one of the controlled variables in Pasteur’s swan neck flask experiment?

<p>The type of broth used (B)</p> Signup and view all the answers

What is the main function of ribosomes within a cell?

<p>To produce proteins (C)</p> Signup and view all the answers

What ingredient is the main reactant required for cellular respiration?

<p>Glucose (D)</p> Signup and view all the answers

Which of the following statements is NOT a point of the cell theory?

<p>All cells can spontaneously generate. (C)</p> Signup and view all the answers

What defines a controlled variable in an experiment?

<p>The variable that remains constant throughout the experiment. (A)</p> Signup and view all the answers

In Louis Pasteur’s experiment on spontaneous generation, what was the key element that proved his hypothesis?

<p>Use of swan-necked flasks. (D)</p> Signup and view all the answers

What is the function of organelles that are exclusively found in plant cells?

<p>To conduct photosynthesis. (D)</p> Signup and view all the answers

If a microscope has an objective lens of 10x and an ocular lens of 45x, what is the total magnification?

<p>450x (D)</p> Signup and view all the answers

Which of the following is NOT considered an organelle found in eukaryotic cells?

<p>Plasmid (C)</p> Signup and view all the answers

What formula is used to compare different magnifications in microscopy?

<p>M1D1 = M2D2 (A)</p> Signup and view all the answers

Which organelle is specifically absent in animal cells compared to plant cells?

<p>Cell wall (A)</p> Signup and view all the answers

Flashcards

Magnification

The ability of a microscope to make an object appear larger.

Objective Lens

The lens closest to the specimen that produces the first magnified image.

Ocular Lens

The lens closest to your eye, which magnifies the image produced by the objective lens.

Total Magnification

The total magnification of a microscope is calculated by multiplying the magnification of the objective lens by the magnification of the ocular lens.

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Cell Theory

All living things are composed of cells. Cells are the basic unit of structure and function in living things. All cells come from preexisting cells.

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Spontaneous Generation

The belief that living things could arise spontaneously from nonliving matter

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Manipulated Variable

The variable that is changed or manipulated by the experimenter.

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Responding Variable

The variable that is measured or observed in an experiment.

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What is magnification?

The apparent size of an object as viewed through a microscope, calculated by multiplying the actual size of the object by the magnification of the microscope.

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What is original size?

The original size of an object is the actual size of the object before it is magnified by a microscope.

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What is Field of View (FOV)?

The field of view (FOV) is the circular area that you can see through the microscope. It changes depending on the magnification level.

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What are the 3 points of cell theory?

The three main tenets of cell theory are: 1. All living organisms are made up of one or more cells. 2. The cell is the basic unit of life. 3. All cells arise from pre-existing cells.

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What is spontaneous generation?

Spontaneous generation is the outdated idea that life could arise from non-living matter.

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How are prokaryotes and eukaryotes different?

Prokaryotes are single-celled organisms that lack a nucleus and other membrane-bound organelles. Eukaryotes are organisms that have a nucleus and other membrane-bound organelles.

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Why do plant cells have chloroplasts and cell walls?

Plant cells have chloroplasts, which are responsible for photosynthesis, and cell walls, which provide structural support. Animal cells lack these organelles.

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Which organelle produces ATP?

Mitochondria are the powerhouses of the cell, responsible for producing ATP through cellular respiration.

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Study Notes

Cell Theory & Microscopy

  • Three main points of cell theory:
    • All living organisms are composed of one or more cells.
    • The cell is the basic unit of structure and organization in organisms.
    • All cells arise from pre-existing cells.

Microscopy Techniques

  • Calculating total magnification:

    • Multiply the objective lens magnification by the ocular lens magnification.
  • Estimating specimen size:

    • Use the formula: FOV diameter / # of specimen across diameter = apparent size of a single specimen
    • Use the formula: M₁D₁ = M₂D₂ to find FOV at different magnifications (Where M1 and D1 are magnification and FOV at one magnification and M2 and D2 are magnification and FOV at the second magnification respectively).

Spontaneous Generation

  • Definition: The idea that living organisms can arise from nonliving matter.
  • Experiments disproving spontaneous generation:
    • Francesco Redi's meat experiment: disproved spontaneous generation of maggots from meat.
    • Louis Pasteur's swan-neck flask experiment: disproved spontaneous generation of microorganisms from broth.

Cell Structure and Function

  • Definition/function of organelles (Organelles: are specialized structures within cells that perform specific functions):

    • Nucleus: Stores the genetic material (DNA).
    • Chloroplast: Conducts photosynthesis in plant cells.
    • Lysosome: Breaks down waste and cellular debris.
    • Endoplasmic Reticulum: Synthesizes proteins and lipids.
    • Plant-specific organelles: chloroplasts, cell wall (starred on diagram)
  • Organelles only in plants:

    • chloroplasts
    • cell walls

Prokaryotic vs. Eukaryotic Cells

  • Differences between prokaryotic and eukaryotic cells
  • Comparisons between prokaryotes and eukaryotes will require more than one bullet point

Cell Research Advancements

  • Examples of cell research advancements:
    • any 3 examples of cell research from the class. Why are they considered advancements? Are there ethical implications/issues/concerns?

Importance of Cell Biology

  • Reason for studying cell biology:
    • Importance for understanding everyday life processes.

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