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Which of the following is NOT an advantage of using a rich protein source in cell culture?
Which of the following is NOT an advantage of using a rich protein source in cell culture?
Cryopreservation allows for the immediate use of all cell lines without the need for storage.
Cryopreservation allows for the immediate use of all cell lines without the need for storage.
False
What is the primary purpose of adding cryoprotectants like DMSO or glycerol during cryopreservation?
What is the primary purpose of adding cryoprotectants like DMSO or glycerol during cryopreservation?
To depress the temperature at which intracellular ice forms.
During cryopreservation, cells should be in the _____ phase before resuspending them with a cryoprotectant.
During cryopreservation, cells should be in the _____ phase before resuspending them with a cryoprotectant.
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Match the cell culture component with its function:
Match the cell culture component with its function:
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What is a characteristic of a finite cell line?
What is a characteristic of a finite cell line?
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A primary cell culture can divide indefinitely and is not susceptible to contact inhibition.
A primary cell culture can divide indefinitely and is not susceptible to contact inhibition.
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What is the primary purpose of creating a histotypic culture?
What is the primary purpose of creating a histotypic culture?
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Cells in a __________ culture are re-aggregated to resemble the original tissue structure.
Cells in a __________ culture are re-aggregated to resemble the original tissue structure.
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Match the type of cell with its characteristic:
Match the type of cell with its characteristic:
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Which method is recommended when only small amounts of tissue are available?
Which method is recommended when only small amounts of tissue are available?
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What type of culture involves the recombination of cells from different lineages?
What type of culture involves the recombination of cells from different lineages?
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What genetic alteration occurs in continuous cell lines?
What genetic alteration occurs in continuous cell lines?
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Enzymatic disaggregation has the advantage of selecting for migratory cells.
Enzymatic disaggregation has the advantage of selecting for migratory cells.
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Suspension cells can survive and proliferate only when attached to a substratum.
Suspension cells can survive and proliferate only when attached to a substratum.
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What is known as complete media?
What is known as complete media?
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The purpose of adding ________ to cell culture media is to monitor pH levels.
The purpose of adding ________ to cell culture media is to monitor pH levels.
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Match the following components of cell culture media with their functions:
Match the following components of cell culture media with their functions:
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What is a major disadvantage of using trypsin in enzymatic disaggregation?
What is a major disadvantage of using trypsin in enzymatic disaggregation?
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Adding amino acids in excess can produce ammonia, which is beneficial to the cells.
Adding amino acids in excess can produce ammonia, which is beneficial to the cells.
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List two components of cell culture media that contribute to regulating physiological conditions.
List two components of cell culture media that contribute to regulating physiological conditions.
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Study Notes
Cell Lines and Cell Strains
- Cells with indefinite potential are called cell lines
- Cell lines arise from primary cell cultures
- Cell strains are subpopulations of a cell line, genetically manipulated
- Primary cells are morphologically similar to the parent tissue and can only divide a limited number of times until they reach confluency
- Contact inhibition is when cells stop growing when they reach confluency
Types of Cell Cultures
- Cell Culture: Cells removed from tissue and disaggregated, either mechanically or enzymatically
- Histotypic Culture: Cells re-aggregated into 3-dimensional structures, mimicking tissue density
- Organotypic Culture: Similar to histotypic, but cells from different lineages are recombined.
Cell Line Types
- Finite Cell Lines: Divide a limited number of times, and typically may acquire a mutation resulting in a continuous cell line
- Continuous Cell Lines: Have unlimited proliferative potential and their behavior in vitro may not reflect their in vivo behavior; frequently correlates with tumorigenicity
Cell Types
- Adherent Cells: Grow by adhering to the surface of the culture vessel
- Suspension Cells: Survive and proliferate without attachment to a surface
Disaggregation Methods
- Fine Dissection: Useful for small tissue samples. Avoids risking cell loss during mechanical or enzymatic disaggregation but may not be ideal for certain tissues
- Enzymatic Disaggregation: Avoids migration selection issues and allows for high cell yield in a short time, but trypsin can damage cells if exposed for too long.
- Mechanical Disaggregation: Useful for large amounts of soft tissue.
Cell Culture Media
- Complete Media: Contains amino acids, vitamins, extra metabolites, lipids, and minerals.
- Components of Media: Inorganic salts, amino acids, carbohydrates, proteins/peptides, fatty acids/lipids, vitamins/trace elements, serum, antibiotics
- Phenol Red: A pH indicator that can change the color of the medium to indicate the pH level
- Glucose: A high concentration of glucose is often present
Cryopreservation
- Purpose: To preserve cells for later use.
- Principle: Cooling cells at a slow rate in the presence of cryoprotectants like DMSO or glycerol, to prevent ice crystal formation within the cells.
- Ideal Conditions: Log phase, 80% confluence before stationary phase, use cryoprotectant (DMSO or glycerol), slow cooling.
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Description
This quiz covers the fundamental concepts of cell lines and cell cultures, including their definitions, types, and characteristics. Explore how cell lines are derived from primary cultures and the distinctions between finite and continuous cell lines. Test your knowledge on the various cell culture methods and their applications in biological research.
