Podcast
Questions and Answers
A cell line is a type of cell culture that can proliferate forever if a suitable fresh medium is not provided.
A cell line is a type of cell culture that can proliferate forever if a suitable fresh medium is not provided.
False
Cell strains have a finite division potential and can proliferate indefinitely.
Cell strains have a finite division potential and can proliferate indefinitely.
False
A primary culture can be subcultured only once to develop cell lines.
A primary culture can be subcultured only once to develop cell lines.
False
Most cell lines are not tumorigenic.
Most cell lines are not tumorigenic.
Signup and view all the answers
Cells derived from a primary cell line are easier to maintain than cell lines.
Cells derived from a primary cell line are easier to maintain than cell lines.
Signup and view all the answers
Primary cell cultures require a nutrient medium containing low amounts of different amino acids, micronutrients and hormones.
Primary cell cultures require a nutrient medium containing low amounts of different amino acids, micronutrients and hormones.
Signup and view all the answers
Primary cell cultures can be efficiently utilized for an unlimited number of passages.
Primary cell cultures can be efficiently utilized for an unlimited number of passages.
Signup and view all the answers
The biological response received from a primary culture is farther from that in an in vivo environment than the response obtained from cell lines.
The biological response received from a primary culture is farther from that in an in vivo environment than the response obtained from cell lines.
Signup and view all the answers
Primary cell culture is the culture of cells that have undergone subculture.
Primary cell culture is the culture of cells that have undergone subculture.
Signup and view all the answers
Mechanical disaggregation is an expensive and time-consuming technique.
Mechanical disaggregation is an expensive and time-consuming technique.
Signup and view all the answers
Enzymatic disaggregation is less efficient than mechanical disaggregation.
Enzymatic disaggregation is less efficient than mechanical disaggregation.
Signup and view all the answers
Trypsin is not commonly used for enzymatic disaggregation.
Trypsin is not commonly used for enzymatic disaggregation.
Signup and view all the answers
Cell lines are used instead of primary cell cultures when primary cell cultures are inexpensive.
Cell lines are used instead of primary cell cultures when primary cell cultures are inexpensive.
Signup and view all the answers
Primary cell culture is a type of cell line.
Primary cell culture is a type of cell line.
Signup and view all the answers
Enzymatic disaggregation does not affect cell viability.
Enzymatic disaggregation does not affect cell viability.
Signup and view all the answers
Primary explant techniques are not used for the development of primary cell cultures.
Primary explant techniques are not used for the development of primary cell cultures.
Signup and view all the answers
The peptides conjugated to a matrix of inert biomaterial can initiate cellular adhesion.
The peptides conjugated to a matrix of inert biomaterial can initiate cellular adhesion.
Signup and view all the answers
The adhesion potential and chemistry of the matrix cannot be controlled.
The adhesion potential and chemistry of the matrix cannot be controlled.
Signup and view all the answers
Growth factors are distributed to cells in an uncontrolled fashion.
Growth factors are distributed to cells in an uncontrolled fashion.
Signup and view all the answers
The chemical nature of the polymeric network does not affect cellular adhesion and growth.
The chemical nature of the polymeric network does not affect cellular adhesion and growth.
Signup and view all the answers
Functionalization is a rare approach in this field.
Functionalization is a rare approach in this field.
Signup and view all the answers
Growth factors can be immobilized over polymeric networks to study and manipulate cells.
Growth factors can be immobilized over polymeric networks to study and manipulate cells.
Signup and view all the answers
Pattern-immobilization is a less reliable approach than other methods.
Pattern-immobilization is a less reliable approach than other methods.
Signup and view all the answers
The movement of proteins is dependent on the concentration gradient in non-diffusion mechanisms.
The movement of proteins is dependent on the concentration gradient in non-diffusion mechanisms.
Signup and view all the answers
The serial cultivation of human diploid cell strains was first described by Hayflick and Moorhead in 1951.
The serial cultivation of human diploid cell strains was first described by Hayflick and Moorhead in 1951.
Signup and view all the answers
Rabies virus can be cultivated in human diploid cell strain WI-38.
Rabies virus can be cultivated in human diploid cell strain WI-38.
Signup and view all the answers
Losee and Ebeling (1914) successfully cultivated human sarcomatous tissue in vitro.
Losee and Ebeling (1914) successfully cultivated human sarcomatous tissue in vitro.
Signup and view all the answers
Earle (1943) produced malignancy in vitro using mouse fibroblast cultures.
Earle (1943) produced malignancy in vitro using mouse fibroblast cultures.
Signup and view all the answers
Gómez-Lechón et al. (2003) used human hepatocytes to study toxicity and drug metabolism.
Gómez-Lechón et al. (2003) used human hepatocytes to study toxicity and drug metabolism.
Signup and view all the answers
MacDonald (1990) developed new cell lines for animal cell biotechnology.
MacDonald (1990) developed new cell lines for animal cell biotechnology.
Signup and view all the answers
Schurr et al. (2009) evaluated StrataGraft, a pathogen-free human skin substitute, in a clinical trial.
Schurr et al. (2009) evaluated StrataGraft, a pathogen-free human skin substitute, in a clinical trial.
Signup and view all the answers
Capes-Davis et al. (2010) reported that all cell lines are free from cross-contamination.
Capes-Davis et al. (2010) reported that all cell lines are free from cross-contamination.
Signup and view all the answers
Phycocyanins have been found to inhibit the growth of human cells in culture.
Phycocyanins have been found to inhibit the growth of human cells in culture.
Signup and view all the answers
The division potential of cell strains is infinite.
The division potential of cell strains is infinite.
Signup and view all the answers
Natural polymers are often used in drug delivery systems.
Natural polymers are often used in drug delivery systems.
Signup and view all the answers
Biomaterials are not used in tissue engineering.
Biomaterials are not used in tissue engineering.
Signup and view all the answers
Primary cell cultures can be used for an unlimited number of passages.
Primary cell cultures can be used for an unlimited number of passages.
Signup and view all the answers
3D cell culture technologies are not useful for creating tissue-like structures in vitro.
3D cell culture technologies are not useful for creating tissue-like structures in vitro.
Signup and view all the answers
Bhatia has not written about pharmaceutical biotechnology.
Bhatia has not written about pharmaceutical biotechnology.
Signup and view all the answers
Biofunctionalized plants are not used as biomaterials for human cell culture.
Biofunctionalized plants are not used as biomaterials for human cell culture.
Signup and view all the answers
Study Notes
Cell Lines and Cell Strains
- A cell line is a permanently established cell culture that will proliferate forever if a suitable fresh medium is provided continuously.
- A cell strain is obtained either from a primary culture or a cell line through selection or cloning of cells with specific properties or characteristics.
- Cell lines are generally immortalized or transformed cells that have lost control over division, often because of mutations or genetic alterations.
Primary Cell Culture
- A primary cell culture is the first culture of cells, tissues, or organs derived directly from an organism.
- Primary cell cultures can be utilized efficiently up to a few passages (2-4), after which the risk of contamination increases.
- Primary cell cultures have the advantage of providing a biological response closer to that in an in vivo environment than cell lines.
Techniques for Primary Cell Culture Development
- Mechanical disaggregation involves slicing or harvesting tissue and subsequent harvesting of spilled-out cells.
- Enzymatic disaggregation involves using enzymes such as trypsin, collagenase, and others to break down fibrous connective tissue and the extracellular matrix.
Cell Adhesion and Growth
- Researchers can develop suitable matrices with controlled adhesion potential and chemistry.
- Growth factors can be immobilized over polymeric networks to study and manipulate cells.
- Pattern immobilization is a more reliable approach, allowing cells to culture in a matrix with a three-dimensional structure mimicking the in vivo environment.
References
- Various references are cited throughout the text, including research papers and book chapters on cell culture, biotechnology, and biomaterials.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz covers the differences between cell lines and cell strains, including their growth potential and characteristics. Learn about the key features of continuous cell lines and finite cell strains.
