Immortalized Cell Lines and Cell Extraction Quiz

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How are cells usually extracted from solid tissues?

Dried and then crushed

Minced and digested with enzymes like trypsin or collagenase (correct)

Fused with embryonic stem cells

Centrifuged directly

What is the benefit of using autologous cells in tissue engineering?

No need for extraction

Higher risk of rejection and pathogen transmission

Lower risk of rejection and pathogen transmission (correct)

Easier availability

What limits the use of autologous cells in tissue engineering for some individuals?

Genetic diseases

Infections from donor sites

Severe burns

Insufficient quantities or inability to harvest cells (correct)

What is the trend in using cells for tissue engineering, as mentioned in the text?

Use of mesenchymal stem cells from bone marrow and fat Signup and view all the answers

What happens to lab cultures of healthy mammalian cells at the Hayflick limit?

They stop dividing and eventually die Signup and view all the answers

Why is it difficult to extract cells from solid tissues?

Solid tissues resist enzymatic digestion Signup and view all the answers

How does the size of the porogen particles impact the scaffold pores?

Increases the porosity Signup and view all the answers

What is the relationship between the polymer to porogen ratio and the porosity of the final structure?

Directly correlated Signup and view all the answers

In the context of scaffold fabrication, what role do sacrificial porogens play?

Create pores by dissolving away Signup and view all the answers

What happens during gelation in polymerization with encapsulated leachable porogens?

Formation of interconnected pores Signup and view all the answers

What is the primary purpose of porogen removal via controlled degradation in scaffold fabrication?

Creating a porous network Signup and view all the answers

What is one of the key purposes of scaffolds in tissue engineering?

Allowing cell attachment and migration Signup and view all the answers

Why is biodegradability an essential factor for scaffolds?

To enable the scaffold to be absorbed by surrounding tissues Signup and view all the answers

What role does injectability play in the clinical use of scaffolds?

Facilitating the application of the scaffold in clinical settings Signup and view all the answers

Why is controlling the 3D environment essential in organ printing for tissue engineering?

To ensure reproducibility of experiments and offer better results Signup and view all the answers

What is the significance of high porosity and adequate pore size in scaffold fabrication?

To facilitate cell seeding and diffusion throughout the whole structure Signup and view all the answers

Why is the fabrication method of solvent casting and particulate leaching (SCPL) preferred for scaffold preparation?

To prepare structures with regular porosity Signup and view all the answers

What technique is commonly used in 3D bioprinting to deposit bioinks layer by layer?

Microfluidic channels Signup and view all the answers

What is the primary purpose of bioprinting tissues and organs?

To research drugs and pills Signup and view all the answers

How do focalized short-pulsed lasers contribute to 3D cell culture and tissue engineering?

By fabricating microfluidic channels Signup and view all the answers

Which technology or method makes it possible to create micrometer-sized cavities in hydrogels for bioprinting?

Femto-pulsed lasers Signup and view all the answers

What provides unprecedented opportunities for cell biology and stem-cell-based tissue engineering according to the text?

Microfluidic channels Signup and view all the answers

In 3D bioprinting, what type of image is used to generate micrometer-sized cavities in hydrogels?

Thresholded binary images Signup and view all the answers

What is the primary benefit of freeze drying mentioned in the text?

Preservation without losing the original shape of the product Signup and view all the answers

What distinguishes electrospinning from traditional fiber production methods?

Utilization of charged polymer solutions Signup and view all the answers

Why does freeze drying result in a high-quality product?

Maintaining the original shape and using low temperatures Signup and view all the answers

What is the key advantage of electrospinning when producing fibers from polymer solutions?

Avoidance of solvent contamination in the final product Signup and view all the answers

In freeze drying, what does sublimation refer to?

Transition of a substance from solid to gas phase Signup and view all the answers

Why is freeze-dried ice cream praised for its quality according to the text?

Maintaining the shape and quality of the original product Signup and view all the answers