Podcast
Questions and Answers
How are cells usually extracted from solid tissues?
How are cells usually extracted from solid tissues?
- Dried and then crushed
- Minced and digested with enzymes like trypsin or collagenase (correct)
- Fused with embryonic stem cells
- Centrifuged directly
What is the benefit of using autologous cells in tissue engineering?
What is the benefit of using autologous cells in tissue engineering?
- No need for extraction
- Higher risk of rejection and pathogen transmission
- Lower risk of rejection and pathogen transmission (correct)
- Easier availability
What limits the use of autologous cells in tissue engineering for some individuals?
What limits the use of autologous cells in tissue engineering for some individuals?
- Genetic diseases
- Infections from donor sites
- Severe burns
- Insufficient quantities or inability to harvest cells (correct)
What is the trend in using cells for tissue engineering, as mentioned in the text?
What is the trend in using cells for tissue engineering, as mentioned in the text?
What happens to lab cultures of healthy mammalian cells at the Hayflick limit?
What happens to lab cultures of healthy mammalian cells at the Hayflick limit?
Why is it difficult to extract cells from solid tissues?
Why is it difficult to extract cells from solid tissues?
How does the size of the porogen particles impact the scaffold pores?
How does the size of the porogen particles impact the scaffold pores?
What is the relationship between the polymer to porogen ratio and the porosity of the final structure?
What is the relationship between the polymer to porogen ratio and the porosity of the final structure?
In the context of scaffold fabrication, what role do sacrificial porogens play?
In the context of scaffold fabrication, what role do sacrificial porogens play?
What happens during gelation in polymerization with encapsulated leachable porogens?
What happens during gelation in polymerization with encapsulated leachable porogens?
What is the primary purpose of porogen removal via controlled degradation in scaffold fabrication?
What is the primary purpose of porogen removal via controlled degradation in scaffold fabrication?
What is one of the key purposes of scaffolds in tissue engineering?
What is one of the key purposes of scaffolds in tissue engineering?
Why is biodegradability an essential factor for scaffolds?
Why is biodegradability an essential factor for scaffolds?
What role does injectability play in the clinical use of scaffolds?
What role does injectability play in the clinical use of scaffolds?
Why is controlling the 3D environment essential in organ printing for tissue engineering?
Why is controlling the 3D environment essential in organ printing for tissue engineering?
What is the significance of high porosity and adequate pore size in scaffold fabrication?
What is the significance of high porosity and adequate pore size in scaffold fabrication?
Why is the fabrication method of solvent casting and particulate leaching (SCPL) preferred for scaffold preparation?
Why is the fabrication method of solvent casting and particulate leaching (SCPL) preferred for scaffold preparation?
What technique is commonly used in 3D bioprinting to deposit bioinks layer by layer?
What technique is commonly used in 3D bioprinting to deposit bioinks layer by layer?
What is the primary purpose of bioprinting tissues and organs?
What is the primary purpose of bioprinting tissues and organs?
How do focalized short-pulsed lasers contribute to 3D cell culture and tissue engineering?
How do focalized short-pulsed lasers contribute to 3D cell culture and tissue engineering?
Which technology or method makes it possible to create micrometer-sized cavities in hydrogels for bioprinting?
Which technology or method makes it possible to create micrometer-sized cavities in hydrogels for bioprinting?
What provides unprecedented opportunities for cell biology and stem-cell-based tissue engineering according to the text?
What provides unprecedented opportunities for cell biology and stem-cell-based tissue engineering according to the text?
In 3D bioprinting, what type of image is used to generate micrometer-sized cavities in hydrogels?
In 3D bioprinting, what type of image is used to generate micrometer-sized cavities in hydrogels?
What is the primary benefit of freeze drying mentioned in the text?
What is the primary benefit of freeze drying mentioned in the text?
What distinguishes electrospinning from traditional fiber production methods?
What distinguishes electrospinning from traditional fiber production methods?
Why does freeze drying result in a high-quality product?
Why does freeze drying result in a high-quality product?
What is the key advantage of electrospinning when producing fibers from polymer solutions?
What is the key advantage of electrospinning when producing fibers from polymer solutions?
In freeze drying, what does sublimation refer to?
In freeze drying, what does sublimation refer to?
Why is freeze-dried ice cream praised for its quality according to the text?
Why is freeze-dried ice cream praised for its quality according to the text?
