29 Questions
How are cells usually extracted from solid tissues?
Minced and digested with enzymes like trypsin or collagenase
What is the benefit of using autologous cells in tissue engineering?
Lower risk of rejection and pathogen transmission
What limits the use of autologous cells in tissue engineering for some individuals?
Insufficient quantities or inability to harvest cells
What is the trend in using cells for tissue engineering, as mentioned in the text?
Use of mesenchymal stem cells from bone marrow and fat
What happens to lab cultures of healthy mammalian cells at the Hayflick limit?
They stop dividing and eventually die
Why is it difficult to extract cells from solid tissues?
Solid tissues resist enzymatic digestion
How does the size of the porogen particles impact the scaffold pores?
Increases the porosity
What is the relationship between the polymer to porogen ratio and the porosity of the final structure?
Directly correlated
In the context of scaffold fabrication, what role do sacrificial porogens play?
Create pores by dissolving away
What happens during gelation in polymerization with encapsulated leachable porogens?
Formation of interconnected pores
What is the primary purpose of porogen removal via controlled degradation in scaffold fabrication?
Creating a porous network
What is one of the key purposes of scaffolds in tissue engineering?
Allowing cell attachment and migration
Why is biodegradability an essential factor for scaffolds?
To enable the scaffold to be absorbed by surrounding tissues
What role does injectability play in the clinical use of scaffolds?
Facilitating the application of the scaffold in clinical settings
Why is controlling the 3D environment essential in organ printing for tissue engineering?
To ensure reproducibility of experiments and offer better results
What is the significance of high porosity and adequate pore size in scaffold fabrication?
To facilitate cell seeding and diffusion throughout the whole structure
Why is the fabrication method of solvent casting and particulate leaching (SCPL) preferred for scaffold preparation?
To prepare structures with regular porosity
What technique is commonly used in 3D bioprinting to deposit bioinks layer by layer?
Microfluidic channels
What is the primary purpose of bioprinting tissues and organs?
To research drugs and pills
How do focalized short-pulsed lasers contribute to 3D cell culture and tissue engineering?
By fabricating microfluidic channels
Which technology or method makes it possible to create micrometer-sized cavities in hydrogels for bioprinting?
Femto-pulsed lasers
What provides unprecedented opportunities for cell biology and stem-cell-based tissue engineering according to the text?
Microfluidic channels
In 3D bioprinting, what type of image is used to generate micrometer-sized cavities in hydrogels?
Thresholded binary images
What is the primary benefit of freeze drying mentioned in the text?
Preservation without losing the original shape of the product
What distinguishes electrospinning from traditional fiber production methods?
Utilization of charged polymer solutions
Why does freeze drying result in a high-quality product?
Maintaining the original shape and using low temperatures
What is the key advantage of electrospinning when producing fibers from polymer solutions?
Avoidance of solvent contamination in the final product
In freeze drying, what does sublimation refer to?
Transition of a substance from solid to gas phase
Why is freeze-dried ice cream praised for its quality according to the text?
Maintaining the shape and quality of the original product
Test your knowledge about immortalized cell lines and the process of cell extraction from fluid and solid tissues. Learn about the Hayflick limit and methods such as centrifugation and enzymatic digestion. Explore topics like laboratory cell cultures and mouse embryonic stem cells.
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