Immortalized Cell Lines and Cell Extraction Quiz
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Questions and Answers

How are cells usually extracted from solid tissues?

  • Dried and then crushed
  • Minced and digested with enzymes like trypsin or collagenase (correct)
  • Fused with embryonic stem cells
  • Centrifuged directly

    • What is the benefit of using autologous cells in tissue engineering?

  • No need for extraction
  • Higher risk of rejection and pathogen transmission
  • Lower risk of rejection and pathogen transmission (correct)
  • Easier availability

    • What limits the use of autologous cells in tissue engineering for some individuals?

  • Genetic diseases
  • Infections from donor sites
  • Severe burns
  • Insufficient quantities or inability to harvest cells (correct)

    • What is the trend in using cells for tissue engineering, as mentioned in the text?

    <p>Use of mesenchymal stem cells from bone marrow and fat</p> Signup and view all the answers

    What happens to lab cultures of healthy mammalian cells at the Hayflick limit?

    <p>They stop dividing and eventually die</p> Signup and view all the answers

    Why is it difficult to extract cells from solid tissues?

    <p>Solid tissues resist enzymatic digestion</p> Signup and view all the answers

    How does the size of the porogen particles impact the scaffold pores?

    <p>Increases the porosity</p> Signup and view all the answers

    What is the relationship between the polymer to porogen ratio and the porosity of the final structure?

    <p>Directly correlated</p> Signup and view all the answers

    In the context of scaffold fabrication, what role do sacrificial porogens play?

    <p>Create pores by dissolving away</p> Signup and view all the answers

    What happens during gelation in polymerization with encapsulated leachable porogens?

    <p>Formation of interconnected pores</p> Signup and view all the answers

    What is the primary purpose of porogen removal via controlled degradation in scaffold fabrication?

    <p>Creating a porous network</p> Signup and view all the answers

    What is one of the key purposes of scaffolds in tissue engineering?

    <p>Allowing cell attachment and migration</p> Signup and view all the answers

    Why is biodegradability an essential factor for scaffolds?

    <p>To enable the scaffold to be absorbed by surrounding tissues</p> Signup and view all the answers

    What role does injectability play in the clinical use of scaffolds?

    <p>Facilitating the application of the scaffold in clinical settings</p> Signup and view all the answers

    Why is controlling the 3D environment essential in organ printing for tissue engineering?

    <p>To ensure reproducibility of experiments and offer better results</p> Signup and view all the answers

    What is the significance of high porosity and adequate pore size in scaffold fabrication?

    <p>To facilitate cell seeding and diffusion throughout the whole structure</p> Signup and view all the answers

    Why is the fabrication method of solvent casting and particulate leaching (SCPL) preferred for scaffold preparation?

    <p>To prepare structures with regular porosity</p> Signup and view all the answers

    What technique is commonly used in 3D bioprinting to deposit bioinks layer by layer?

    <p>Microfluidic channels</p> Signup and view all the answers

    What is the primary purpose of bioprinting tissues and organs?

    <p>To research drugs and pills</p> Signup and view all the answers

    How do focalized short-pulsed lasers contribute to 3D cell culture and tissue engineering?

    <p>By fabricating microfluidic channels</p> Signup and view all the answers

    Which technology or method makes it possible to create micrometer-sized cavities in hydrogels for bioprinting?

    <p>Femto-pulsed lasers</p> Signup and view all the answers

    What provides unprecedented opportunities for cell biology and stem-cell-based tissue engineering according to the text?

    <p>Microfluidic channels</p> Signup and view all the answers

    In 3D bioprinting, what type of image is used to generate micrometer-sized cavities in hydrogels?

    <p>Thresholded binary images</p> Signup and view all the answers

    What is the primary benefit of freeze drying mentioned in the text?

    <p>Preservation without losing the original shape of the product</p> Signup and view all the answers

    What distinguishes electrospinning from traditional fiber production methods?

    <p>Utilization of charged polymer solutions</p> Signup and view all the answers

    Why does freeze drying result in a high-quality product?

    <p>Maintaining the original shape and using low temperatures</p> Signup and view all the answers

    What is the key advantage of electrospinning when producing fibers from polymer solutions?

    <p>Avoidance of solvent contamination in the final product</p> Signup and view all the answers

    In freeze drying, what does sublimation refer to?

    <p>Transition of a substance from solid to gas phase</p> Signup and view all the answers

    Why is freeze-dried ice cream praised for its quality according to the text?

    <p>Maintaining the shape and quality of the original product</p> Signup and view all the answers

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