Podcast
Questions and Answers
What is the primary purpose of subculturing cells in cell culture?
What is the primary purpose of subculturing cells in cell culture?
- To increase the number of cells and prolong the cell line's lifespan. (correct)
- To genetically modify the cells for specific research purposes.
- To remove dead cells and debris from the culture.
- To induce cell differentiation into specialized cell types.
Which of the following is NOT a typical characteristic of immortalized cell lines?
Which of the following is NOT a typical characteristic of immortalized cell lines?
- Genetic instability.
- Altered morphology compared to primary cells.
- Exhibit contact inhibition. (correct)
- Ability to proliferate indefinitely.
A researcher is working with a newly established cell line and wants to ensure its identity. Which method would be most appropriate for verifying the cell line's species of origin?
A researcher is working with a newly established cell line and wants to ensure its identity. Which method would be most appropriate for verifying the cell line's species of origin?
- Karyotyping
- Growth curve analysis
- Short Tandem Repeat (STR) analysis (correct)
- Morphological assessment
What is the key difference between a primary cell culture and a cell line?
What is the key difference between a primary cell culture and a cell line?
Which of the following cell types is most likely to be anchorage-dependent?
Which of the following cell types is most likely to be anchorage-dependent?
A researcher observes that their cell culture is growing at a slower rate than usual and suspects contamination. Which of the following is the MOST likely cause?
A researcher observes that their cell culture is growing at a slower rate than usual and suspects contamination. Which of the following is the MOST likely cause?
During cell cloning, what is the purpose of limiting dilution?
During cell cloning, what is the purpose of limiting dilution?
Which method is commonly used to synchronize cells at the G1/S phase boundary?
Which method is commonly used to synchronize cells at the G1/S phase boundary?
What does the name 'HeLa' signify in the context of cell lines?
What does the name 'HeLa' signify in the context of cell lines?
A researcher needs to assess the cytotoxic effects of a new drug on a cell culture. Which assay would be most appropriate for quantifying cell death?
A researcher needs to assess the cytotoxic effects of a new drug on a cell culture. Which assay would be most appropriate for quantifying cell death?
Flashcards
Tissue Isolation
Tissue Isolation
The process of taking cells from an animal or plant and growing them in an artificial environment.
Primary Cell Culture Steps
Primary Cell Culture Steps
- Tissue Dissection. 2. Cell Dissociation. 3. Cell Suspension. 4. Plating and Incubation. 5. Media Changes.
Subculture (Passaging)
Subculture (Passaging)
Transferring cells to a new culture vessel with fresh medium to propagate the cell line.
Cell Lines
Cell Lines
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Cell Line Nomenclature
Cell Line Nomenclature
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Cell Line Designation
Cell Line Designation
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Routine Cell Maintenance
Routine Cell Maintenance
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Cell Immortalization
Cell Immortalization
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Cell Transformation
Cell Transformation
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Cell Cloning
Cell Cloning
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Study Notes
- Primary cell culture involves isolating tissue and culturing cells outside their original environment.
- Subculturing enables cell propagation and the creation of cell lines.
- Cell lines are cultures of cells with a indefinite lifespan.
- Cell lines are named using specific nomenclature and designations.
- Routine maintenance is essential for keeping cell cultures healthy.
- Cell line immortalization gives cells an unlimited lifespan.
- Cell transformation changes cell properties, sometimes leading to tumor-like behavior.
- Cell cloning enables creating identical cell populations.
- Cell separation techniques isolate specific cell types from a mixed population.
- Cell synchronization aligns cells in the same cell cycle stage.
- Epithelial, mesenchymal, and tumor cells each require distinct culture conditions.
Measurement of Viability and Cytotoxicity
- Cell viability assays determine the number of living cells in a culture.
- Cytotoxicity assays measure the extent of cell damage or death caused by a substance.
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