Cell Culture Techniques

What is the primary purpose of using special media with nutrients in cell culture?

Which application of cell culture is most directly related to assessing the safety of new pharmaceuticals?

In genetic engineering, why is cell culture utilized?

What is a key characteristic of 'tissue culture'?

What is the critical feature differentiating primary cell cultures from other cell cultures?

What typically happens to primary cell cultures over time?

Which of the following is NOT a characteristic of primary cell cultures?

What is the primary method for purifying primary cells after tissue disaggregation?

Secondary cell cultures are derived from which source?

What is a defining characteristic of secondary cell cultures compared to primary cell cultures?

What is a common method for isolating secondary cell cultures?

Which of the following features distinguishes continuous cell cultures from primary and secondary cell cultures?

How are continuous cell lines typically created?

What is a major characteristic of continuous cell lines regarding their genetic stability?

Which characteristic is commonly associated with continuous cell lines?

What type of cell morphology is characterized by cells attached to a substrate, appearing flattened and polygonal?

Which cell type typically remains in suspension with a spherical shape, not attaching to a substrate?

What is a defining characteristic of fibroblast-like cells in terms of their attachment and shape?

What is the main purpose of a CO2 incubator in cell culture?

Why is sterility an essential requirement in cell culture?

What is the role of phenol red in cell culture media?

Why are antibiotics and antimycotics used in cell culture?

What is the primary function of the basal media in cell culture?

What is the role of L-glutamine in cell culture media?

What is the purpose of trypsinizing cells during cell passaging?

Why is it important to resuspend cells in serum-containing media after trypsinization?

Why is DMSO used in cryopreservation?

What is the typical temperature used for freezing cells for long-term storage?

What is the correct order of steps when passaging cells?

During cell thawing, what is the appropriate temperature for the water bath used to thaw the cells?

What is the primary reason for using a hemocytometer in cell culture?

What is the role of fetal bovine serum (FBS) in cell culture media?

Which of the following is a disadvantage of using fetal bovine serum (FBS) in cell culture?

What is a key consideration in the use of antibiotics in cell culture?

Which of the following is correct regarding the temperature requirements for culturing cells from warm-blooded animals?

What is 'in vivo' in the context of cell culture?

When isolating primary cell cultures directly from tissue, what is a common first step?

A researcher observes that their continuous cell line is growing at an abnormally rapid rate and no longer adheres to the culture flask. Based on the characteristics of continuous cell lines, what is the most likely explanation?

A researcher is working with a novel cell line and observes that the cells are growing well but are also rapidly undergoing genetic drift. To mitigate this issue, what strategy should the researcher prioritize?

A lab technician consistently struggles with bacterial contamination in cell cultures despite following standard aseptic techniques. The technician meticulously cleans the laminar flow hood, sterilizes all equipment, and uses antibiotics in the media. However, contamination persists. What is the MOST likely overlooked source of contamination?

A researcher aims to establish a 3D cell culture model to mimic the in vivo environment of a tumor more accurately. They decide to use a scaffold-free method. Which of the following is the MOST significant limitation they might encounter, compared to scaffold-based methods?

Within the context of cell culture, what does 'in vitro' refer to?

Which of the following is a typical application of cell culture in cancer research?

What is a key characteristic that distinguishes continuous cell cultures from primary and secondary cell cultures?

What is a primary reason for using a laminar flow hood in cell culture?

What is the primary function of serum, such as fetal bovine serum (FBS), in cell culture media?

During the passaging process, why is it important to wash cells with PBS (phosphate-buffered saline) before adding trypsin?

What is the purpose of using DMSO (dimethyl sulfoxide) in the cryopreservation of cells?

Which characteristic is commonly associated with continuous cell lines that may impact experimental reproducibility?

What is the underlying reason for the finite lifespan of primary cell cultures?

A researcher is attempting to establish a long-term 3D culture of primary hepatocytes to study chronic liver disease, but faces significant challenges in maintaining their differentiated function and preventing rapid phenotypic drift. Which of the following strategies would MOST likely address BOTH of these challenges simultaneously?