Cell and Tissue Culture Techniques
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Questions and Answers

What are tissue and cell culture techniques primarily used for?

  • To repair damaged tissues in living organisms
  • To understand the mechanical properties of organs
  • To create synthetic biological materials
  • To analyze the progression of cancer (correct)
  • Which term refers to the techniques that replicate the natural environment of cells within a living organism?

  • In-vitro techniques
  • Ex-vivo techniques
  • Bioengineering techniques
  • In-vivo techniques (correct)
  • What significant contribution did Robert Hooke make to cell biology?

  • He named the cells after the rooms of monks (correct)
  • He discovered the nucleus of the cell
  • He identified living cells in human tissue
  • He invented the microscope
  • What is the role of nuclear staining in cytology?

    <p>To differentiate cells from debris and bacteria</p> Signup and view all the answers

    Which structure within a cell is primarily responsible for housing genetic material?

    <p>Nucleus</p> Signup and view all the answers

    What is the significance of using DAPI in cell staining?

    <p>It stains the nuclei to identify cells clearly</p> Signup and view all the answers

    What is an important aspect of combining engineering, biology, and medicine in the context of tissue culture?

    <p>To fabricate devices for diagnosing and curing diseases</p> Signup and view all the answers

    Which technique would NOT be classified as a part of tissue and cell culture?

    <p>Genetic engineering techniques</p> Signup and view all the answers

    What is one of the main challenges in studying cancer through cell culture techniques?

    <p>It is difficult to replicate the complexity of human tissues</p> Signup and view all the answers

    How do tissue and cell culture techniques aid in cancer diagnosis and treatment?

    <p>They enable the examination of cellular responses to therapeutics</p> Signup and view all the answers

    What does the term 'bioreactor' refer to in tissue engineering?

    <p>A device that provides a controlled environment for cell culture</p> Signup and view all the answers

    What is a microfilament involved in, within the structure of a cell?

    <p>Providing structural support and shape</p> Signup and view all the answers

    In what context are oral cells relevant to cytology?

    <p>They are extracted for studies on oral cancer</p> Signup and view all the answers

    Study Notes

    Cell and Tissue Culture Techniques

    • Cell Culture is a combination of engineering, biology, and medicine, used to study and manipulate cells in a controlled environment.

    • In-vivo techniques involve studying cells and tissues within a living organism.

    • Ex-vivo techniques involve studying cells and tissues outside of a living organism, but in a more natural environment.

    • In-vitro techniques involve studying cells and tissues in a completely artificial environment, such as a petri dish.

    • Cancer is a disease caused by abnormal cell division.

    • Cell Engineering is a field that focuses on the manipulation and growth of cells for various purposes, including disease modeling and therapeutic development.

    • Robert Hooke (1665) first observed cells using a microscope, naming them after the small rooms monks lived in.

    • Cytology is the study of cells, including their structure and function.

    • Nuclear staining is a technique used to visualize cell nuclei, helping to distinguish between cells and debris.

    • DAPI is a common nuclear stain that makes the nucleus appear blue.

    • Microscopy is essential for observing the structure and function of cells and tissues.

    • Bioreactors are engineered systems used to control and monitor cell growth and development.

    • Tissue Engineering focuses on the creation of functional tissues and organs from cells.

    • Therapeutic and diagnostic devices are being developed using cell culture techniques to address cancer and other diseases.

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    Description

    Explore the various methods of cell and tissue culture techniques, including in-vivo, ex-vivo, and in-vitro approaches. Understand the significance of cell engineering and cytology in studying cancer and manipulating cells for therapeutic purposes. Delve into historical contributions to the field, such as Robert Hooke's discovery of cells.

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