Cell Culture Techniques Quiz
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Questions and Answers

What did the creation of genetically altered cell lines through mutagenesis and cloning allow for in the study of cells in culture?

  • The examination of differentiated functions characteristic of their cell type of origin.
  • The growth of normal and transformed human cells in vitro.
  • Direct comparison of cells from normal and transformed tissues. (correct)
  • The study of cellular physiology and metabolism.

    • What was a significant outcome of the establishment of cells in culture as cell lines?

  • An arbitrary mixture of amino acids, vitamins, co-factors, carbohydrates, and salts.
  • The production and secretion of hormones and other differentiated functions in vitro. (correct)
  • The reliance on complex tissue extracts and clots for cell growth.
  • A limited study of growth and response to hormones and environmental factors.

    • How did Harry Eagle's demonstration impact the study of cellular physiology and metabolism?

  • It was limited to the growth of normal and transformed human cells in vitro.
  • It focused on the examination of differentiated functions characteristic of their cell type of origin.
  • It revealed that serum protein was necessary for cell growth in culture.
  • It allowed for the manipulation of cells in a variety of new ways. (correct)

    • What did the growth of normal and transformed human cells in vitro allow researchers to do?

    <p>Study growth and response to hormones and other environmental factors.</p> Signup and view all the answers

    What did the maintenance of differentiated functions characteristic of their cell type of origin allow for in the study of cell lines?

    <p>The examination of differentiated functions characteristic of their cell type of origin.</p> Signup and view all the answers

    What was a key opportunity created by Harry Eagle's demonstration regarding tissue culture?

    <p>The replacement of complex tissue extracts with an arbitrary mixture of compounds.</p> Signup and view all the answers

    What new area of cell culture did Harry Eagle's demonstration open up?

    <p>The opportunity to manipulate cells in a variety of new ways</p> Signup and view all the answers

    What did the creation of cell lines maintaining differentiated functions allow the study of?

    <p>The study of growth and response to hormones and other environmental factors, and the production and secretion of hormones and other differentiated functions in vitro</p> Signup and view all the answers

    What were the outcomes of the growth of normal and transformed human cells in vitro?

    <p>Allowed researchers to study the growth and response to hormones and other environmental factors, and the production and secretion of hormones and other differentiated functions in vitro</p> Signup and view all the answers

    What significant opportunities did the establishment of cells in culture as cell lines create?

    <p>The study of growth and response to hormones and other environmental factors, and the production and secretion of hormones and other differentiated functions in vitro</p> Signup and view all the answers

    What did the creation of genetically altered cell lines through mutagenesis and cloning allow for in the study of cells in culture?

    <p>The creation of genetically altered cell lines allowed for the study of direct comparison of cells from normal and transformed tissues, and the study of cellular physiology and metabolism</p> Signup and view all the answers

    How did Harry Eagle's demonstration impact the study of cellular physiology and metabolism?

    <p>It allowed for the study of cellular physiology and metabolism in new ways</p> Signup and view all the answers

    What was a key opportunity created by Harry Eagle's demonstration regarding tissue culture?

    <p>The opportunity to manipulate cells in a variety of new ways</p> Signup and view all the answers

    What was the impact of Harry Eagle's demonstration on the study of cellular physiology and metabolism?

    <p>It opened up a new area of cell culture, allowing the manipulation of cells in a variety of new ways, including the study of cellular physiology and metabolism.</p> Signup and view all the answers

    What significant opportunities did the establishment of cells in culture as cell lines create?

    <p>It allowed the study of growth and response to hormones and other environmental factors, and the production and secretion of hormones and other differentiated functions in vitro.</p> Signup and view all the answers

    How did the growth of normal and transformed human cells in vitro impact researchers?

    <p>It allowed researchers to study the growth and response of human cells to different environmental factors and to produce and secrete hormones and other differentiated functions in vitro.</p> Signup and view all the answers

    What did the creation of genetically altered cell lines through mutagenesis and cloning allow for in the study of cells in culture?

    <p>It allowed for the direct comparison of cells from normal and transformed tissues, and the study of cellular physiology and metabolism.</p> Signup and view all the answers

    What was a key opportunity created by Harry Eagle's demonstration regarding tissue culture?

    <p>The opportunity to manipulate cells in a variety of new ways, including creating genetically altered cell lines through mutagenesis and cloning, and the direct comparison of cells from normal and transformed tissues.</p> Signup and view all the answers

    What did the creation of cell lines maintaining differentiated functions allow the study of?

    <p>It allowed the study of growth and response to hormones and other environmental factors, and the production and secretion of hormones and other differentiated functions in vitro.</p> Signup and view all the answers

    What did Harry Eagle's demonstration reveal about tissue culture?

    <p>It revealed that an arbitrary mixture of nutrients, supplemented with a small amount of serum protein, could replace complex tissue extracts previously used to grow cells, opening up a new area of cell culture.</p> Signup and view all the answers

    Study Notes

    Cell Culture and Cellular Physiology

    • The creation of genetically altered cell lines through mutagenesis and cloning allowed for the study of cellular physiology and metabolism at the molecular level.

    Impact of Harry Eagle's Demonstration

    • Harry Eagle's demonstration impacted the study of cellular physiology and metabolism by enabling the growth of cells in culture.
    • His demonstration opened up a new area of cell culture, allowing for the study of cellular physiology and metabolism in vitro.

    Cell Lines and Differentiation

    • The creation of cell lines maintaining differentiated functions allowed for the study of cellular physiology and metabolism in context of specific cell types.
    • The maintenance of differentiated functions characteristic of their cell type of origin enabled researchers to study cellular processes in relation to specific cell types.

    Outcomes of Cell Culture

    • The growth of normal and transformed human cells in vitro allowed researchers to study cellular physiology and metabolism in a controlled environment.
    • The growth of normal and transformed human cells in vitro enabled the study of cellular processes and mechanisms underlying cellular transformation.

    Opportunities Created

    • The establishment of cells in culture as cell lines created opportunities for studying cellular physiology and metabolism in a controlled environment.
    • Harry Eagle's demonstration created opportunities for studying tissue culture and its applications in cellular physiology and metabolism.

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    Description

    Test your knowledge about the history and techniques of cell culture, including the groundbreaking work by Harry Eagle in 1955. Explore the principles of tissue culture and its impact on developmental biology.

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