Cell and Tissue Culture Course Overview

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Questions and Answers

Which of the following is NOT one of the three major types of cell culture?

Cell line

Primary cell culture

Secondary cell culture

Tertiary cell culture (correct)

Primary cell culture refers to cells that are derived from a single cell or tissue source and are maintained in vitro.

True

What is the difference between finite and continuous cell lines?

Finite cell lines have a limited lifespan while continuous cell lines can divide indefinitely.

The process of introducing foreign DNA into a cell line is called __________.

transfection Signup and view all the answers

Match the following cell culture types with their definitions:

Primary Cell Culture = Cells derived directly from a tissue source. Secondary Cell Culture = Cultivation of cells derived from a primary culture. Cell Line = A population of cells that can divide indefinitely. Continuous Cell Line = Cells that can proliferate endlessly in culture. Signup and view all the answers

Which category includes the cells grown from a primary culture after subculturing?

Secondary cell culture Signup and view all the answers

All cell lines derived from primary cultures can undergo transformation.

False Signup and view all the answers

Name one major characteristic of primary cell cultures.

They have a limited lifespan and will not proliferate indefinitely. Signup and view all the answers

What is the primary purpose of sub-culturing in tissue culture?

To transfer cells to a fresh growth medium Signup and view all the answers

Primary cell culture can remain viable for an extended period without any nutrient replenishment.

False Signup and view all the answers

What is the term for the culture formed after sub-culturing of primary cell culture?

Secondary cell culture Signup and view all the answers

Sub-culturing is also referred to as _______.

passaging Signup and view all the answers

What occurs during the log phase of cell culture?

Cells proliferate exponentially Signup and view all the answers

Match the following terms with their definitions:

Primary cell culture = Stage of culture after isolation of cells Secondary cell culture = Culture formed after sub-culturing Sub-culturing = Removal of cells into a fresh medium Contact inhibition = Cells stop growing when they reach a certain density Signup and view all the answers

A secondary cell culture exhibits contact inhibition.

True Signup and view all the answers

What is the finite nature of primary cell culture?

Limited life span in vitro Signup and view all the answers

What is the main characteristic of a continuous cell line?

It can proliferate indefinitely. Signup and view all the answers

Finite cell lines can proliferate indefinitely.

False Signup and view all the answers

What is the process called when a finite cell line becomes capable of indefinite division?

Transformation Signup and view all the answers

A primary culture is derived from ______ material.

in vivo Signup and view all the answers

Which term describes the maintenance of tissue explants in culture?

Tissue culture Signup and view all the answers

What triggers senescence in normal cells?

Genetically determined event Signup and view all the answers

Contact inhibition is a mechanism that promotes cell growth by encouraging collisions.

False Signup and view all the answers

Study Notes

Cell and Tissue Culture Course

The course is titled "Cell and Tissue Culture Course"
Course code is SCBZ 432
Instructor is Dr. Ghadeer Alrefaei
Year is 2014
University is University of Jeddah

Cell Culture Types

There are three major types of cell culture: primary, secondary, and cell line
Primary cell culture occurs after isolation of cells from tissue
Cells are proliferated under suitable conditions and occupy the available substrate
This stage proceeds to secondary culture or subculture
Primary cell culture is a stage after isolation but before the cells become a cell line
After the final subculture it becomes a cell line
Secondary cell culture (or subculture):
Occurs when cells occupy all available substrate in adherent cultures
Or when cells in suspension cultures exceed the growth capability in the culture medium
Cell proliferation is reduced or stops
Needed to keep cells at an optimal density
Needed to stimulate further proliferation and divide cells and supply fresh medium
Sub-culturing is also known as passaging
Characteristics of Primary cell culture
Derived from primary cell culture and isolated by selection or cloning
Becoming a more homogeneous cell population
Having finite life span in vitro
Retaining differentiated phenotype
Mainly anchorage-dependent exhibiting contact inhibition

Cell Line

A permanently established cell culture
Will proliferate indefinitely given appropriate medium and space
Developed from a single cell consisting of cells with uniform genetic makeup
A cell population derived from primary cell line
Continuous cell lines have unlimited lifespan
Finite cell lines have a limited lifespan

Continuous Cell Lines

Normal cells typically divide a limited number of times before losing the ability to proliferate
This genetically determined event is known as senescence
Some cell lines become immortal via transformation
Can be either spontaneous, chemical, or viral
When a finite/normal cell line undergoes transformation, it acquires the ability to divide indefinitely, becoming a continuous cell line

Other Important Terminology

Tissue culture: Maintenance of tissue explants in culture
Cell culture: Maintenance of dissociated cells in culture
Clone: The progeny isolated from a single cell
Immortalization: Enabling cells to extend their life in culture
Contact inhibition: Growth mechanism where cells attempt to move when they collide, avoiding further collisions
Passage/Subculture: Sub-culturing of cells from on container to another which enables further propagation of cell line or cell strain
Sub-culturing of cells from a previous culture into a fresh medium which allows for propagation of the cell line

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Description

This quiz covers the fundamentals of cell and tissue culture, focusing on the different types such as primary, secondary, and cell line cultures. It explains the processes involved in cell isolation and proliferation, as well as the significance of each culture stage. Perfect for students in SCBZ 432 at the University of Jeddah.