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Questions and Answers
Which of the following is NOT one of the three major types of cell culture?
Which of the following is NOT one of the three major types of cell culture?
- Cell line
- Primary cell culture
- Secondary cell culture
- Tertiary cell culture (correct)
Primary cell culture refers to cells that are derived from a single cell or tissue source and are maintained in vitro.
Primary cell culture refers to cells that are derived from a single cell or tissue source and are maintained in vitro.
True (A)
What is the difference between finite and continuous cell lines?
What is the difference between finite and continuous cell lines?
Finite cell lines have a limited lifespan while continuous cell lines can divide indefinitely.
The process of introducing foreign DNA into a cell line is called __________.
The process of introducing foreign DNA into a cell line is called __________.
Match the following cell culture types with their definitions:
Match the following cell culture types with their definitions:
Which category includes the cells grown from a primary culture after subculturing?
Which category includes the cells grown from a primary culture after subculturing?
All cell lines derived from primary cultures can undergo transformation.
All cell lines derived from primary cultures can undergo transformation.
Name one major characteristic of primary cell cultures.
Name one major characteristic of primary cell cultures.
What is the primary purpose of sub-culturing in tissue culture?
What is the primary purpose of sub-culturing in tissue culture?
Primary cell culture can remain viable for an extended period without any nutrient replenishment.
Primary cell culture can remain viable for an extended period without any nutrient replenishment.
What is the term for the culture formed after sub-culturing of primary cell culture?
What is the term for the culture formed after sub-culturing of primary cell culture?
Sub-culturing is also referred to as _______.
Sub-culturing is also referred to as _______.
What occurs during the log phase of cell culture?
What occurs during the log phase of cell culture?
Match the following terms with their definitions:
Match the following terms with their definitions:
A secondary cell culture exhibits contact inhibition.
A secondary cell culture exhibits contact inhibition.
What is the finite nature of primary cell culture?
What is the finite nature of primary cell culture?
What is the main characteristic of a continuous cell line?
What is the main characteristic of a continuous cell line?
Finite cell lines can proliferate indefinitely.
Finite cell lines can proliferate indefinitely.
What is the process called when a finite cell line becomes capable of indefinite division?
What is the process called when a finite cell line becomes capable of indefinite division?
A primary culture is derived from ______ material.
A primary culture is derived from ______ material.
Which term describes the maintenance of tissue explants in culture?
Which term describes the maintenance of tissue explants in culture?
What triggers senescence in normal cells?
What triggers senescence in normal cells?
Contact inhibition is a mechanism that promotes cell growth by encouraging collisions.
Contact inhibition is a mechanism that promotes cell growth by encouraging collisions.
Flashcards
Primary cell culture
Primary cell culture
Cultures generated directly from tissues or organs of an organism.
Finite cell lines
Finite cell lines
Cells that have been isolated and grown in vitro for a limited number of generations.
Continuous cell lines
Continuous cell lines
Cells that have undergone genetic alterations that allow them to proliferate indefinitely in culture.
Cell culture
Cell culture
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Transfection
Transfection
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Transformation
Transformation
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Subculture
Subculture
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Tissue culture
Tissue culture
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Primary culture
Primary culture
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Cell line
Cell line
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Immortalization
Immortalization
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Clone
Clone
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Contact inhibition
Contact inhibition
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Secondary cell culture
Secondary cell culture
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Cell strain
Cell strain
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Log phase
Log phase
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Lag phase
Lag phase
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Study Notes
Cell and Tissue Culture Course
- The course is titled "Cell and Tissue Culture Course"
- Course code is SCBZ 432
- Instructor is Dr. Ghadeer Alrefaei
- Year is 2014
- University is University of Jeddah
Cell Culture Types
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There are three major types of cell culture: primary, secondary, and cell line
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Primary cell culture occurs after isolation of cells from tissue
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Cells are proliferated under suitable conditions and occupy the available substrate
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This stage proceeds to secondary culture or subculture
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Primary cell culture is a stage after isolation but before the cells become a cell line
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After the final subculture it becomes a cell line
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Secondary cell culture (or subculture):
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Occurs when cells occupy all available substrate in adherent cultures
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Or when cells in suspension cultures exceed the growth capability in the culture medium
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Cell proliferation is reduced or stops
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Needed to keep cells at an optimal density
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Needed to stimulate further proliferation and divide cells and supply fresh medium
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Sub-culturing is also known as passaging
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Characteristics of Primary cell culture
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Derived from primary cell culture and isolated by selection or cloning
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Becoming a more homogeneous cell population
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Having finite life span in vitro
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Retaining differentiated phenotype
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Mainly anchorage-dependent exhibiting contact inhibition
Cell Line
- A permanently established cell culture
- Will proliferate indefinitely given appropriate medium and space
- Developed from a single cell consisting of cells with uniform genetic makeup
- A cell population derived from primary cell line
- Continuous cell lines have unlimited lifespan
- Finite cell lines have a limited lifespan
Continuous Cell Lines
- Normal cells typically divide a limited number of times before losing the ability to proliferate
- This genetically determined event is known as senescence
- Some cell lines become immortal via transformation
- Can be either spontaneous, chemical, or viral
- When a finite/normal cell line undergoes transformation, it acquires the ability to divide indefinitely, becoming a continuous cell line
Other Important Terminology
- Tissue culture: Maintenance of tissue explants in culture
- Cell culture: Maintenance of dissociated cells in culture
- Clone: The progeny isolated from a single cell
- Immortalization: Enabling cells to extend their life in culture
- Contact inhibition: Growth mechanism where cells attempt to move when they collide, avoiding further collisions
- Passage/Subculture: Sub-culturing of cells from on container to another which enables further propagation of cell line or cell strain
- Sub-culturing of cells from a previous culture into a fresh medium which allows for propagation of the cell line
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