Cell Culture Techniques and Viability Assessment Quiz

To prepare and delineate cell culture and perform viability of cells using various dyes such as Trypan blue, eosin, methylene blue, nigrosin, and fast green.

Only non-viable cells take up the dye and appear blue, while viable cells remain round and clear without absorbing the dye.

Male diafiltration is performed to prevent viable cells from absorbing the dye and appearing non-viable, as Trypan blue has a greater affinity for serum proteins than for cellular membranes.

<p>Trypan blue dye is used to distinguish viable cells from non-viable cells, as only non-viable cells take up the dye and appear blue.</p> Signup and view all the answers

<p>The use of Trypan blue dye is time-sensitive, and it may lead to false results if not handled carefully.</p> Signup and view all the answers