40 Questions
What is the purpose of using trypsin in cell culture?
To detach cells from the surface of the dish
What is the primary reason for using aseptic conditions in cell culture?
To prevent contamination by yeast and fungi
What is the main difference between Level 2 and Level 3 bio safety levels?
The type of pathogens being worked with
What is the purpose of a bioreactor in industrial scale cell culture?
To grow human cells on a large scale
What is the ideal temperature for incubating cells in culture?
37 degrees Celsius
Why are antibiotics often added to culture media?
To prevent contamination by bacteria
What is the purpose of passaging cells in culture?
To split cells into new dishes when they run out of space
What is the primary function of a laminar flow cabinet in cell culture?
To maintain aseptic conditions
What is the primary difference between Level 1 and Level 4 bio safety levels?
The type of pathogens being worked with
Why are cells grown in suspension used in industrial scale cell culture?
To increase the yield of vaccine production
What is a characteristic of cells isolated from tissues?
They are genetically diverse.
What is the primary reason why isolated cells are difficult to culture?
Stress from the removal from their natural environment.
What is the Hayflick limit in relation to eukaryotic cells?
A limited lifespan in culture.
What is the purpose of telomeres in cell division?
To protect chromosomes from degradation.
What is the main difference between senescent cells and cells that are not dividing?
Senescent cells are permanently stuck in G0 phase and cannot enter the cell cycle.
What is unique about tumour cells?
They can rebuild their telomeres using the enzyme telomerase.
What was the significance of the HeLa cells?
They were the first human cell line that was immortal.
What is the purpose of PHE and ATCC?
To store and provide cell lines for research.
How many human cell lines are currently available?
Over 4,000.
How many species have cell lines available?
Over 150.
What is the primary reason why isolated cells are sensitive to their environment?
They lack essential nutrients
What happens to cells that are not dividing?
They enter G0 phase
What is the significance of telomere shortening?
It limits the number of cell divisions
What is unique about HeLa cells?
They are immortal and can be cultured indefinitely
What is the purpose of PHE and ATCC?
To provide a repository for cell lines
What is the Hayflick limit related to?
Lifespan of eukaryotic cells in culture
What is the main difference between senescent cells and cells in G0 phase?
Senescent cells cannot enter cell cycle
What is the significance of the telomerase enzyme in tumour cells?
It allows tumour cells to rebuild their telomeres
What is the primary reason why tumour cells are immortal?
They can rebuild their telomeres
What is the significance of HeLa cells in the development of the polio vaccine?
They were used to test the efficacy of the vaccine
What is the primary purpose of using antibiotics in cell culture media?
To prevent bacterial contamination and promote aseptic conditions
What is a characteristic of cells that do not need to attach to a surface for growth?
They are typically grown in suspension
What is the primary difference between Level 1 and Level 2 bio safety levels?
The presence of a physical containment device
What is the purpose of using aseptic conditions in cell culture?
To prevent bacterial contamination and promote cell health
What is the primary reason for passaging cells in culture?
To prevent cells from running out of space and nutrients
What is the primary function of trypsin in cell culture?
To detach cells from the surface of the dish
What is the primary difference between industrial scale cell culture and laboratory scale cell culture?
The scale of cell growth and expansion
What is the primary reason for using a controlled access system in bio safety levels?
To prevent unauthorized access to the laboratory
What is the primary function of an autoclave in bio safety levels?
To sterilize equipment and materials
What is the primary difference between cells grown in suspension and cells that attach to a surface?
The requirement for a surface for growth
Learn about the challenges of culturing cells, including their genetic diversity, susceptibility to stress, and limited lifespan in culture. Understand the Hayflick limit and its implications for cell culture.
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