Podcast
Questions and Answers
What is the purpose of using trypsin in cell culture?
What is the purpose of using trypsin in cell culture?
- To prevent bacterial contamination
- To induce cell differentiation
- To add nutrients to the culture media
- To detach cells from the surface of the dish (correct)
What is the primary reason for using aseptic conditions in cell culture?
What is the primary reason for using aseptic conditions in cell culture?
- To reduce the risk of biohazard exposure
- To promote cell growth and division
- To maintain a consistent pH in the media
- To prevent contamination by yeast and fungi (correct)
What is the main difference between Level 2 and Level 3 bio safety levels?
What is the main difference between Level 2 and Level 3 bio safety levels?
- The type of pathogens being worked with (correct)
- The use of personal protective equipment
- The use of a autoclave
- The presence of a shower between doors
What is the purpose of a bioreactor in industrial scale cell culture?
What is the purpose of a bioreactor in industrial scale cell culture?
What is the ideal temperature for incubating cells in culture?
What is the ideal temperature for incubating cells in culture?
Why are antibiotics often added to culture media?
Why are antibiotics often added to culture media?
What is the purpose of passaging cells in culture?
What is the purpose of passaging cells in culture?
What is the primary function of a laminar flow cabinet in cell culture?
What is the primary function of a laminar flow cabinet in cell culture?
What is the primary difference between Level 1 and Level 4 bio safety levels?
What is the primary difference between Level 1 and Level 4 bio safety levels?
Why are cells grown in suspension used in industrial scale cell culture?
Why are cells grown in suspension used in industrial scale cell culture?
What is a characteristic of cells isolated from tissues?
What is a characteristic of cells isolated from tissues?
What is the primary reason why isolated cells are difficult to culture?
What is the primary reason why isolated cells are difficult to culture?
What is the Hayflick limit in relation to eukaryotic cells?
What is the Hayflick limit in relation to eukaryotic cells?
What is the purpose of telomeres in cell division?
What is the purpose of telomeres in cell division?
What is the main difference between senescent cells and cells that are not dividing?
What is the main difference between senescent cells and cells that are not dividing?
What is unique about tumour cells?
What is unique about tumour cells?
What was the significance of the HeLa cells?
What was the significance of the HeLa cells?
What is the purpose of PHE and ATCC?
What is the purpose of PHE and ATCC?
How many human cell lines are currently available?
How many human cell lines are currently available?
How many species have cell lines available?
How many species have cell lines available?
What is the primary reason why isolated cells are sensitive to their environment?
What is the primary reason why isolated cells are sensitive to their environment?
What happens to cells that are not dividing?
What happens to cells that are not dividing?
What is the significance of telomere shortening?
What is the significance of telomere shortening?
What is unique about HeLa cells?
What is unique about HeLa cells?
What is the purpose of PHE and ATCC?
What is the purpose of PHE and ATCC?
What is the Hayflick limit related to?
What is the Hayflick limit related to?
What is the main difference between senescent cells and cells in G0 phase?
What is the main difference between senescent cells and cells in G0 phase?
What is the significance of the telomerase enzyme in tumour cells?
What is the significance of the telomerase enzyme in tumour cells?
What is the primary reason why tumour cells are immortal?
What is the primary reason why tumour cells are immortal?
What is the significance of HeLa cells in the development of the polio vaccine?
What is the significance of HeLa cells in the development of the polio vaccine?
What is the primary purpose of using antibiotics in cell culture media?
What is the primary purpose of using antibiotics in cell culture media?
What is a characteristic of cells that do not need to attach to a surface for growth?
What is a characteristic of cells that do not need to attach to a surface for growth?
What is the primary difference between Level 1 and Level 2 bio safety levels?
What is the primary difference between Level 1 and Level 2 bio safety levels?
What is the purpose of using aseptic conditions in cell culture?
What is the purpose of using aseptic conditions in cell culture?
What is the primary reason for passaging cells in culture?
What is the primary reason for passaging cells in culture?
What is the primary function of trypsin in cell culture?
What is the primary function of trypsin in cell culture?
What is the primary difference between industrial scale cell culture and laboratory scale cell culture?
What is the primary difference between industrial scale cell culture and laboratory scale cell culture?
What is the primary reason for using a controlled access system in bio safety levels?
What is the primary reason for using a controlled access system in bio safety levels?
What is the primary function of an autoclave in bio safety levels?
What is the primary function of an autoclave in bio safety levels?
What is the primary difference between cells grown in suspension and cells that attach to a surface?
What is the primary difference between cells grown in suspension and cells that attach to a surface?
