Cell Culture and Limitations
40 Questions
3 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of using trypsin in cell culture?

  • To prevent bacterial contamination
  • To induce cell differentiation
  • To add nutrients to the culture media
  • To detach cells from the surface of the dish (correct)

    • What is the primary reason for using aseptic conditions in cell culture?

  • To reduce the risk of biohazard exposure
  • To promote cell growth and division
  • To maintain a consistent pH in the media
  • To prevent contamination by yeast and fungi (correct)

    • What is the main difference between Level 2 and Level 3 bio safety levels?

  • The type of pathogens being worked with (correct)
  • The use of personal protective equipment
  • The use of a autoclave
  • The presence of a shower between doors

    • What is the purpose of a bioreactor in industrial scale cell culture?

    <p>To grow human cells on a large scale</p> Signup and view all the answers

    What is the ideal temperature for incubating cells in culture?

    <p>37 degrees Celsius</p> Signup and view all the answers

    Why are antibiotics often added to culture media?

    <p>To prevent contamination by bacteria</p> Signup and view all the answers

    What is the purpose of passaging cells in culture?

    <p>To split cells into new dishes when they run out of space</p> Signup and view all the answers

    What is the primary function of a laminar flow cabinet in cell culture?

    <p>To maintain aseptic conditions</p> Signup and view all the answers

    What is the primary difference between Level 1 and Level 4 bio safety levels?

    <p>The type of pathogens being worked with</p> Signup and view all the answers

    Why are cells grown in suspension used in industrial scale cell culture?

    <p>To increase the yield of vaccine production</p> Signup and view all the answers

    What is a characteristic of cells isolated from tissues?

    <p>They are genetically diverse.</p> Signup and view all the answers

    What is the primary reason why isolated cells are difficult to culture?

    <p>Stress from the removal from their natural environment.</p> Signup and view all the answers

    What is the Hayflick limit in relation to eukaryotic cells?

    <p>A limited lifespan in culture.</p> Signup and view all the answers

    What is the purpose of telomeres in cell division?

    <p>To protect chromosomes from degradation.</p> Signup and view all the answers

    What is the main difference between senescent cells and cells that are not dividing?

    <p>Senescent cells are permanently stuck in G0 phase and cannot enter the cell cycle.</p> Signup and view all the answers

    What is unique about tumour cells?

    <p>They can rebuild their telomeres using the enzyme telomerase.</p> Signup and view all the answers

    What was the significance of the HeLa cells?

    <p>They were the first human cell line that was immortal.</p> Signup and view all the answers

    What is the purpose of PHE and ATCC?

    <p>To store and provide cell lines for research.</p> Signup and view all the answers

    How many human cell lines are currently available?

    <p>Over 4,000.</p> Signup and view all the answers

    How many species have cell lines available?

    <p>Over 150.</p> Signup and view all the answers

    What is the primary reason why isolated cells are sensitive to their environment?

    <p>They lack essential nutrients</p> Signup and view all the answers

    What happens to cells that are not dividing?

    <p>They enter G0 phase</p> Signup and view all the answers

    What is the significance of telomere shortening?

    <p>It limits the number of cell divisions</p> Signup and view all the answers

    What is unique about HeLa cells?

    <p>They are immortal and can be cultured indefinitely</p> Signup and view all the answers

    What is the purpose of PHE and ATCC?

    <p>To provide a repository for cell lines</p> Signup and view all the answers

    What is the Hayflick limit related to?

    <p>Lifespan of eukaryotic cells in culture</p> Signup and view all the answers

    What is the main difference between senescent cells and cells in G0 phase?

    <p>Senescent cells cannot enter cell cycle</p> Signup and view all the answers

    What is the significance of the telomerase enzyme in tumour cells?

    <p>It allows tumour cells to rebuild their telomeres</p> Signup and view all the answers

    What is the primary reason why tumour cells are immortal?

    <p>They can rebuild their telomeres</p> Signup and view all the answers

    What is the significance of HeLa cells in the development of the polio vaccine?

    <p>They were used to test the efficacy of the vaccine</p> Signup and view all the answers

    What is the primary purpose of using antibiotics in cell culture media?

    <p>To prevent bacterial contamination and promote aseptic conditions</p> Signup and view all the answers

    What is a characteristic of cells that do not need to attach to a surface for growth?

    <p>They are typically grown in suspension</p> Signup and view all the answers

    What is the primary difference between Level 1 and Level 2 bio safety levels?

    <p>The presence of a physical containment device</p> Signup and view all the answers

    What is the purpose of using aseptic conditions in cell culture?

    <p>To prevent bacterial contamination and promote cell health</p> Signup and view all the answers

    What is the primary reason for passaging cells in culture?

    <p>To prevent cells from running out of space and nutrients</p> Signup and view all the answers

    What is the primary function of trypsin in cell culture?

    <p>To detach cells from the surface of the dish</p> Signup and view all the answers

    What is the primary difference between industrial scale cell culture and laboratory scale cell culture?

    <p>The scale of cell growth and expansion</p> Signup and view all the answers

    What is the primary reason for using a controlled access system in bio safety levels?

    <p>To prevent unauthorized access to the laboratory</p> Signup and view all the answers

    What is the primary function of an autoclave in bio safety levels?

    <p>To sterilize equipment and materials</p> Signup and view all the answers

    What is the primary difference between cells grown in suspension and cells that attach to a surface?

    <p>The requirement for a surface for growth</p> Signup and view all the answers

    More Like This

    Cell Biology Growth and HeLa Cells
    5 questions

    Cell Biology Growth and HeLa Cells

    UnforgettableDune avatar
    UnforgettableDune
    Cell Biology: Degradation and Preparations
    29 questions

    Cell Biology: Degradation and Preparations

    JovialEiffelTower avatar
    JovialEiffelTower
    SIO2004 Animal Cell Culture Lecture 5
    21 questions

    SIO2004 Animal Cell Culture Lecture 5

    MagnificentBinomial3316 avatar
    MagnificentBinomial3316
    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser