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Questions and Answers
What is the main purpose of administering magnesium sulfate in this study?
What is the main purpose of administering magnesium sulfate in this study?
- To analyze the nutrient content in feces
- To increase water absorption in the intestine
- To enhance carmine absorption
- To observe its cathartic effect (correct)
How does magnesium sulfate exert its cathartic effect in the intestine?
How does magnesium sulfate exert its cathartic effect in the intestine?
- By stimulating mucus production in the intestine
- By increasing the absorption of magnesium ions
- By promoting digestion and nutrient breakdown
- By creating high osmotic pressure and inhibiting water absorption (correct)
What was the method used for administering the carmine solution to mice?
What was the method used for administering the carmine solution to mice?
- Subcutaneous injection
- Intramuscular injection
- Oral ingestion from a bowl
- Syringe-fed intragastric gavage (correct)
Which of the following best describes the measurement used to assess the advance percentage of carmine?
Which of the following best describes the measurement used to assess the advance percentage of carmine?
What characterizes the fecal form and features after administration of magnesium sulfate?
What characterizes the fecal form and features after administration of magnesium sulfate?
Flashcards
Cathartic Effect of Magnesium Sulfate
Cathartic Effect of Magnesium Sulfate
Magnesium sulfate's mechanism of action as a laxative involves increasing osmotic pressure in the intestines, reducing water absorption, and promoting bowel movements. This is due to the high concentration of magnesium and sulfate ions, effectively drawing water into the intestinal lumen and triggering peristalsis.
Advance Percentage of Carmine
Advance Percentage of Carmine
The advancement percentage of carmine in the intestine is a measure of how far a dye (carmine) travels through the bowel. It helps assess the speed and effectiveness of intestinal movement.
Fecal Traits
Fecal Traits
The physical characteristics of feces, such as solidity, shape, and consistency, are observed to evaluate the effectiveness of the cathartic effect.
Intragastric Administration by Gavage Method
Intragastric Administration by Gavage Method
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Experimental Procedure of Cathartic Effect
Experimental Procedure of Cathartic Effect
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Study Notes
Cathartic Effect of Magnesium Sulfate
- Purpose: Observe the cathartic effect of magnesium sulfate and its mechanism.
- Mechanism: Magnesium sulfate, when administered orally, disrupts water absorption in the intestines. Magnesium and sulfate ions create a high osmotic pressure, leading to increased intestinal lumen volume. This causes intestinal peristalsis, resulting in a cathartic effect.
Observation Content
- Advance Percentage of Carmine: Calculated by dividing the distance carmine travels in the bowel by the total intestinal length, then multiplying by 100%. This quantifies the movement of the carmine dye as a measure of intestinal motility.
- Fecal Form and Features: Observations include fecal traits for analysis such as solid/shapeless, sparse/dense
Materials, Methods, and Procedure
- Materials: Mice, carmine solution, syringe, scissors, electronic balance, carmine-magnesium sulfate solutions.
- Experimental Procedure (First Step): Mice are selected, weighed, labeled (with picric acid) and divided into groups. Two mice per group.
- Experimental Procedure (Second Step): One group (A) receives a carmine-saline solution. A second group (B) receives carmine-magnesium sulfate solution. The administration is done orally.
- Intragastric Administration Method: Mice are fed via intragastric gavage. A feeding needle is placed into the stomach via the pharynx and esophagus.
- Observation After Drug Treatment (30 min): Mice are killed by cervical dislocation. Abdomens are opened. Peristalsis and intestinal expansion are observed. The intestines are removed and the distance of carmine movement is measured (from pylorus to the beginning of carmine and to the end).
- Third Step: Data on carmine advancement and fecal characteristics are recorded, and analyzed to understand the cathartic effect of magnesium sulfate.
Data Collection
- Table Cathartic Effect of Magnesium Sulfate: A table is provided for gathering data on the advance percentage of carmine and fecal traits concerning the two groups (carmine solution treatment and carmine-magnesium sulfate treatment).
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