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Questions and Answers
What is the primary mechanism by which magnesium sulfate exerts its cathartic effect?
What is the primary mechanism by which magnesium sulfate exerts its cathartic effect?
- It creates high osmotic pressure, inhibiting water absorption. (correct)
- It directly stimulates the nervous system to enhance bowel movements.
- It increases the absorption of water in the intestine.
- It induces nausea, causing spontaneous elimination.
Which indicator is used to measure the effectiveness of magnesium sulfate's cathartic effect?
Which indicator is used to measure the effectiveness of magnesium sulfate's cathartic effect?
- The time taken for initial bowel movement.
- Weight loss over a specified period.
- Advance percentage of carmine in the bowel. (correct)
- The fecal volume produced.
What method is used for administering the carmine solutions to the mice?
What method is used for administering the carmine solutions to the mice?
- Oral administration without syringes.
- Subcutaneous injection.
- Intramuscular injection.
- Syringe-fed intragastric administration. (correct)
What is the expected change in the fecal form when magnesium sulfate is administered?
What is the expected change in the fecal form when magnesium sulfate is administered?
What is the main purpose of administering different solutions to group A and group B in the experiment?
What is the main purpose of administering different solutions to group A and group B in the experiment?
Flashcards
How does magnesium sulfate work as a laxative?
How does magnesium sulfate work as a laxative?
Magnesium sulfate exerts its laxative effect by increasing osmotic pressure in the intestines.
What does the 'advance percentage of carmine' measure?
What does the 'advance percentage of carmine' measure?
The advance percentage of carmine is a measurement of how far the dye travels through the intestines, indicating the speed of intestinal transit.
What information does the 'Fecal traits' category in the table provide?
What information does the 'Fecal traits' category in the table provide?
The 'Fecal traits' category in the table describes the physical characteristics of the feces, such as consistency, shape, and whether it is formed or loose.
What is 'intragastric gavage'?
What is 'intragastric gavage'?
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What is the main purpose of the experiment?
What is the main purpose of the experiment?
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Study Notes
Cathartic Effect of Magnesium Sulfate
- Purpose: Observe the cathartic effect of magnesium sulfate and its mechanism.
- Mechanism: Magnesium ions (Mg2+) and sulfate ions, after oral administration, create a high osmotic pressure in the intestines. This inhibits water absorption, expands intestinal lumen volume, and promotes peristalsis, resulting in a cathartic effect.
Observation Content
- Advance Percentage of Carmine: Calculated by dividing the distance carmine traveled in the bowel by the total intestinal length, then multiplying by 100%. This measures the speed of intestinal movement.
- Fecal Form and Features: Observations include whether feces are solid, shaped, sparse, or shapeless.
Materials, Methods, and Procedure
- Materials: Mice, carmine solution, syringes, scissors, electronic balance, carmine-magnesium sulphate solution.
- Experimental Procedure:
- Step 1: Select and weigh two mice per group, labeling them A and B with picric acid.
- Step 2: Administer treatments. Group A receives 0.8ml carmine-saline solution. Group B receives 0.8ml carmine-magnesium sulfate solution.
- Method of Administration: Intragastric gavage using a ball-tipped feeding needle. The needle is placed into the stomach through the pharynx and esophagus along the oral palate.
- Step 3: Record data and analyze the cathartic effect over 30 minutes.
Observation After Drug Treatment
- Procedure: Kill mice via cervical dislocation. Cut the abdomen, expose the intestines, observe peristalsis and intestinal expansion. Remove and measure intestines. Determine the distances from the pylorus to the front and end of the carmine dye.
- Calculation: Determine the advance percentage of carmine (I/L x 100%), where I is the distance to the front of the dye and L is the distance to the end of the intestine.
Data Analysis
- Table: The table will record the results of the advance percentage of carmine and fecal traits for each group.
- Key Point: Intragastric administration via gavage is crucial for the experimental procedure.
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