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Questions and Answers
If positive cultures are obtained after the third challenge, a higher concentration of the disinfectant may be tested.
If positive cultures are obtained after the third challenge, a higher concentration of the disinfectant may be tested.
True
In the surface disinfection test, the number of survivors is determined by spraying the carrier with a disinfectant.
In the surface disinfection test, the number of survivors is determined by spraying the carrier with a disinfectant.
False
The control series in the surface disinfection test involves substituting the disinfectant with vinegar.
The control series in the surface disinfection test involves substituting the disinfectant with vinegar.
False
Surface tests cannot reflect real-life conditions like contact times, temperatures, use-dilutions, and surface properties.
Surface tests cannot reflect real-life conditions like contact times, temperatures, use-dilutions, and surface properties.
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The surface disinfection test evaluates the effectiveness of the agent against surface-adhered microbes.
The surface disinfection test evaluates the effectiveness of the agent against surface-adhered microbes.
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Complicated practical tests under real-life conditions are considered an advantage of the disinfectant testing procedure.
Complicated practical tests under real-life conditions are considered an advantage of the disinfectant testing procedure.
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In the Capacity Dilution Test, disinfectant is challenged repeatedly by successive additions of fungal suspension until its capacity to kill has been exhausted.
In the Capacity Dilution Test, disinfectant is challenged repeatedly by successive additions of fungal suspension until its capacity to kill has been exhausted.
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The Capacity Dilution Test, also known as the Kelsey Sykes test, determines the concentration of disinfectant needed to be effective in clean and dirty conditions.
The Capacity Dilution Test, also known as the Kelsey Sykes test, determines the concentration of disinfectant needed to be effective in clean and dirty conditions.
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For clean conditions in the Capacity Dilution Test, dilutions of disinfectant are made in yeast suspension.
For clean conditions in the Capacity Dilution Test, dilutions of disinfectant are made in yeast suspension.
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Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas vulgaris, and Escherichia coli are the organisms selected for the Capacity Dilution Test.
Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas vulgaris, and Escherichia coli are the organisms selected for the Capacity Dilution Test.
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The contact time of disinfectant and organism in the Capacity Dilution Test is 10 minutes.
The contact time of disinfectant and organism in the Capacity Dilution Test is 10 minutes.
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A negative result in the Capacity Dilution Test is recorded when sets contain two or more positive cultures.
A negative result in the Capacity Dilution Test is recorded when sets contain two or more positive cultures.
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Study Notes
Disinfectant Testing
- If positive cultures are obtained after the third challenge, a higher concentration of the disinfectant may be tested.
- The surface disinfection test determines the number of survivors by spraying the carrier with a disinfectant.
- In the control series of the surface disinfection test, the disinfectant is substituted with vinegar.
- Surface tests have limitations, such as inability to reflect real-life conditions like contact times, temperatures, use-dilutions, and surface properties.
Capacity Dilution Test (Kelsey Sykes Test)
- The Capacity Dilution Test evaluates the effectiveness of a disinfectant by challenging it repeatedly with successive additions of fungal suspension until its capacity to kill has been exhausted.
- The test determines the concentration of disinfectant needed to be effective in both clean and dirty conditions.
- In clean conditions, dilutions of disinfectant are made in yeast suspension.
- The test organisms used are Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas vulgaris, and Escherichia coli.
- The contact time of disinfectant and organism in the test is 10 minutes.
- A negative result is recorded when sets contain two or more positive cultures.
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Description
Learn about the capacity dilution test, which evaluates the ability of a disinfectant to maintain its efficacy in the presence of increasing levels of bacteria and dirt. The Kelsey Sykes test is a well-known capacity test often used in housekeeping and instrument disinfection scenarios.
