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If positive cultures are obtained after the third challenge, a higher concentration of the disinfectant may be tested.
True
In the surface disinfection test, the number of survivors is determined by spraying the carrier with a disinfectant.
False
The control series in the surface disinfection test involves substituting the disinfectant with vinegar.
False
Surface tests cannot reflect real-life conditions like contact times, temperatures, use-dilutions, and surface properties.
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The surface disinfection test evaluates the effectiveness of the agent against surface-adhered microbes.
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Complicated practical tests under real-life conditions are considered an advantage of the disinfectant testing procedure.
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In the Capacity Dilution Test, disinfectant is challenged repeatedly by successive additions of fungal suspension until its capacity to kill has been exhausted.
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The Capacity Dilution Test, also known as the Kelsey Sykes test, determines the concentration of disinfectant needed to be effective in clean and dirty conditions.
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For clean conditions in the Capacity Dilution Test, dilutions of disinfectant are made in yeast suspension.
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Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas vulgaris, and Escherichia coli are the organisms selected for the Capacity Dilution Test.
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The contact time of disinfectant and organism in the Capacity Dilution Test is 10 minutes.
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A negative result in the Capacity Dilution Test is recorded when sets contain two or more positive cultures.
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Study Notes
Disinfectant Testing
- If positive cultures are obtained after the third challenge, a higher concentration of the disinfectant may be tested.
- The surface disinfection test determines the number of survivors by spraying the carrier with a disinfectant.
- In the control series of the surface disinfection test, the disinfectant is substituted with vinegar.
- Surface tests have limitations, such as inability to reflect real-life conditions like contact times, temperatures, use-dilutions, and surface properties.
Capacity Dilution Test (Kelsey Sykes Test)
- The Capacity Dilution Test evaluates the effectiveness of a disinfectant by challenging it repeatedly with successive additions of fungal suspension until its capacity to kill has been exhausted.
- The test determines the concentration of disinfectant needed to be effective in both clean and dirty conditions.
- In clean conditions, dilutions of disinfectant are made in yeast suspension.
- The test organisms used are Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas vulgaris, and Escherichia coli.
- The contact time of disinfectant and organism in the test is 10 minutes.
- A negative result is recorded when sets contain two or more positive cultures.
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Description
Learn about the capacity dilution test, which evaluates the ability of a disinfectant to maintain its efficacy in the presence of increasing levels of bacteria and dirt. The Kelsey Sykes test is a well-known capacity test often used in housekeeping and instrument disinfection scenarios.
