Disinfectant Resistance and Sterilization Methods

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Questions and Answers

What is the primary reason for flushing the handpiece with water between patients?

  • To flush out the sprayed lubricant inside the handpiece
  • To flush out the bacterial spores that have lodged inside the turbines, air, and water lines
  • To flush out patient fluids that might have entered the turbines, air, and water lines (correct)
  • To flush out the sterilant that went inside the handpiece

Which sterilization method is capable of sterilizing the entire dental operatory simultaneously?

  • Dry heat
  • Glutaraldehyde
  • Autoclave (correct)
  • UV sterilization

What is a significant advantage of using dry heat sterilization?

  • Lower temperature
  • Shorter cycle time
  • May process wet instruments
  • Does not dull cutting edges of instruments (correct)

What is NOT a rationale for flushing a handpiece with water?

<p>To remove air from the handpiece (D)</p> Signup and view all the answers

Which of the following is least effective for immediate sterilization in a dental setting?

<p>Quaternary ammonium compound (D)</p> Signup and view all the answers

Which of the following organisms is most resistant to disinfectants?

<p>Bacillus atrophaeus (D)</p> Signup and view all the answers

What classification does a dental mouth mirror fall under?

<p>Semicritical instrument (C)</p> Signup and view all the answers

Which sterilization method and cycle temperature correlation is incorrect?

<p>Autoclave: 130°C (D)</p> Signup and view all the answers

What is the recommended sterilization method for heat-resistant critical and semicritical instruments?

<p>Autoclave (A)</p> Signup and view all the answers

Why should instruments be dried before sterilizing under Chemiclave?

<p>To prevent corrosion of metal instruments (C)</p> Signup and view all the answers

What is a disadvantage of steam sterilization?

<p>Both A &amp; B (D)</p> Signup and view all the answers

Which chemical is described as a one-minute sterilant against Mycobacterium tuberculosis?

<p>Glutaraldehyde (C)</p> Signup and view all the answers

What is the reason for using nitrile or butyl rubber gloves when handling glutaraldehyde?

<p>It is toxic to the skin and can cause blistering (C)</p> Signup and view all the answers

Flashcards

Why flush handpieces?

Flushing handpieces between patients removes potential contaminants like sprayed lubricant, sterilant residues, bacterial spores, and patient fluids.

What sterilizes the whole operatory?

Autoclaves use high heat and pressure to sterilize instruments by killing all microorganisms.

Advantage of dry heat sterilization?

Dry heat sterilization is gentle on instruments, meaning it preserves sharp edges.

What does dry heat sterilize?

Dry heat sterilization is effective against a wide range of microorganisms, including bacterial spores.

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Disadvantage of Dry Heat?

Dry heat sterilization requires a longer cycle time compared to other methods like autoclaving.

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Which organism is most resistant to disinfectants?

Mycobacterium tuberculosis is extremely resistant to disinfectants. It's a tough bacterium, often encountered in healthcare settings.

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What classification is a dental mouth mirror?

Dental mouth mirrors are considered semicritical instruments as they come into contact with mucous membranes but do not penetrate tissues.

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Matching Sterilization Methods & Temperatures

Autoclave uses high-pressure steam at 130°C to sterilize. Dry heat requires 180°C. Glass bead sterilization operates at 370°C. Chemiclave uses a chemical solution at 180°C.

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Best Sterilization for Heat-resistant Instruments

Autoclave is the preferred method for sterilizing heat-resistant, critical and semicritical instruments due to its effectiveness and safety.

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Why dry instruments before Chemiclave?

Drying instruments before sterilization in Chemiclave prevents corrosion and ensures drying at the end of the cycle.

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Disadvantages of Steam Sterilization

Steam sterilization can dull sharp instruments and leaves them wet at the end of the cycle.

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Which sterilant is quick against Mycobacterium tuberculosis?

Glutaraldehyde is a powerful sterilant that can kill Mycobacterium tuberculosis in a single minute.

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How does UV sterilization work?

UV sterilization destroys microorganisms by damaging their DNA. This method utilizes ultraviolet light to inactivate pathogens.

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Study Notes

Disinfectant Resistance

  • Bacillus atrophaeus is most resistant to disinfectants among the listed options.

Dental Mouth Mirror Classification

  • Dental mouth mirrors are classified as semicritical instruments.

Incorrect Sterilization Method/Temperature Correlation

  • The incorrect correlation is Chemiclave: 180°C.

Sterilization Method for Heat-Resistant Instruments

  • Autoclave is the sterilization method of choice for heat-resistant, critical, and semicritical instruments.

Chemiclave Drying Rationale

  • Drying instruments before Chemiclave sterilization is crucial to ensure proper instrument dryness at the end of the cycle and prevent corrosion of metal instruments.

Disadvantages of Steam Sterilization

  • Steam sterilization may dull sharp instruments and instruments are typically wet at the end of the sterilization cycle.

One-Minute Sterilant for Mycobacterium tuberculosis

  • Glutaraldehyde is a one-minute sterilant against Mycobacterium tuberculosis.

DNA Inactivation Method

  • Chemiclave (and UV sterilization, among others) destroys microorganisms by inactivating their DNA.

Glutaraldehyde Glove Rationale

  • Nitrile or butyl rubber gloves should be worn when handling glutaraldehyde to protect against its toxicity, pungent smell, potential staining, and skin blistering risks.

Dry Heat Sterilization Prohibited Items

  • Cloth, surgical burs, dental mouth mirrors, dental explorers should not be placed in a dry heat sterilizer.

Sterilization Method for Remote Missions

  • For remote dental missions, autoclave is the best sterilization choice due to its dependability.

Handpiece Flushing Time

  • The recommended handpiece flushing time between patients is 20-30 seconds.

Handpiece Flushing Rationale

  • Flushing the handpiece with water between patients helps remove sprayed lubricant, patient fluids, sterilant, and bacterial spores.

Operatory-Wide Sterilization

  • Quaternary ammonium compound and UV sterilization are NOT suitable for sterilizing the entire operatory at once. Autoclaves can be adapted, depending on the size of the operatory.

Dry Heat Sterilization Advantages

  • Dry heat sterilization does not dull cutting edges, has a shorter cycle time, and uses a lower temperature than steam sterilization, and can handle instruments that are already dry.

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