Blood Collection and Analysis

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Questions and Answers

Why are collection tubes often color-coded?

  • To specify the patient's blood type.
  • To denote the additives present in the tube. (correct)
  • To signify the laboratory performing the test.
  • To indicate the expiration date of the tube.

What is the typical pH range of blood?

  • 6.85-7.25
  • 7.35-7.45 (correct)
  • 7.00-7.30
  • 7.50-7.60

In venipuncture, which vein is most commonly used?

  • Basilic vein
  • Femoral vein
  • Medial cubital vein (correct)
  • Cephalic vein

What is the approximate total blood volume in adult males?

<p>5-6 Liters (D)</p> Signup and view all the answers

What effect does the presence of EDTA have on a blood sample?

<p>Inhibits clotting (D)</p> Signup and view all the answers

How does blood color relate to its oxygen content?

<p>Blood is bright red when oxygenated and dark red/purple when deoxygenated. (B)</p> Signup and view all the answers

What percentage of body mass does blood approximately account for?

<p>8% (C)</p> Signup and view all the answers

Which characteristic distinguishes blood from water?

<p>Higher density (D)</p> Signup and view all the answers

For what purpose would a procoagulant tube be used?

<p>To obtain a serum sample by allowing the blood to clot. (D)</p> Signup and view all the answers

Which additive prevents coagulation?

<p>Heparin (B)</p> Signup and view all the answers

Which of the following describes the relative viscosity of blood?

<p>More viscous than water. (D)</p> Signup and view all the answers

How much of the extracellular fluid does blood account for?

<p>20% (C)</p> Signup and view all the answers

If blood is bright red during blood collection, what does this indicate about the blood's characteristics?

<p>High oxygen content (A)</p> Signup and view all the answers

Why is it important to select the correct blood collection tube for specific laboratory tests?

<p>To prevent interference from additives that could affect test results. (C)</p> Signup and view all the answers

What is the primary function of citrate as an additive in blood collection tubes?

<p>To prevent blood clotting. (C)</p> Signup and view all the answers

Which of the following is a key characteristic of blood that affects its flow properties?

<p>Viscosity (D)</p> Signup and view all the answers

How does the use of collection tubes with anticoagulants impact downstream laboratory testing?

<p>It allows for the analysis of whole blood or plasma. (C)</p> Signup and view all the answers

When might a phlebotomist choose a vein other than the medial cubital vein for venipuncture?

<p>If the medial cubital vein is inaccessible or unsuitable. (C)</p> Signup and view all the answers

What consideration is most important when choosing the correct order of draw for multiple blood collection tubes?

<p>Preventing cross-contamination of additives between tubes. (B)</p> Signup and view all the answers

What percentage of total body weight does extracellular fluid (ECF) typically constitute in a 70kg person?

<p>20% (A)</p> Signup and view all the answers

Which of the following characteristics describes the composition of intracellular fluid (ICF)?

<p>High in potassium and magnesium, low in sodium and chloride (B)</p> Signup and view all the answers

Which of the following is a key difference between plasma and interstitial fluid?

<p>Plasma has a higher protein concentration than interstitial fluid. (A)</p> Signup and view all the answers

What is the approximate volume of blood in a 70kg person, based on the rule of thumb provided?

<p>4.9 Liters (C)</p> Signup and view all the answers

What is the primary purpose of using an anticoagulant, such as heparin or EDTA, when collecting blood into a tube?

<p>To prevent the blood from clotting. (A)</p> Signup and view all the answers

After centrifuging a tube of blood, what percentage of the total blood volume does the plasma typically represent?

<p>55% (D)</p> Signup and view all the answers

How does the water content in the body typically differ between males and females?

<p>Females have a lower percentage of body weight as water due to higher fat content. (B)</p> Signup and view all the answers

Which of the following is a characteristic of blood plasma?

<p>Is a clear, straw-colored liquid. (C)</p> Signup and view all the answers

What is the approximate volume of transcellular fluid in a 70kg person?

<p>0.8L (B)</p> Signup and view all the answers

Given that a typical 70kg male contains approximately 42L of water, what percentage of his total body weight does this water represent?

<p>60% (A)</p> Signup and view all the answers

What are the main components that blood centrifugation separates?

<p>Cellular Components and Fluid (A)</p> Signup and view all the answers

Which formed element makes up the highest percentage of whole blood?

<p>Erythrocytes (D)</p> Signup and view all the answers

What is the main difference in function between blood and lymph?

<p>Blood bathes all cells including Lymph, whereas Lymph has a similar composition to plasma except it contains low protein. (A)</p> Signup and view all the answers

Which of the following parameters is lower in the fluid outside of cells?

<p>$[K^+]$ (A)</p> Signup and view all the answers

Which of the following parameters describes intracellular fluid?

<p>Approx. 10^14 cells (A)</p> Signup and view all the answers

What does the presence of buffy coat comprised leukocytes and platelets typically present following centrifugation of blood?

<p>less than 1% of whole blood (A)</p> Signup and view all the answers

Which of the following blood components contains albumin, globulins, fibrinogen, electrolytes, gases, regulatory substances, nutrients and waste products?

<p>Plasma (B)</p> Signup and view all the answers

Under normal circumstances, which of the following solutes would be found in slightly higher concentrations in plasma than in interstitial fluid?

<p>Proteins (C)</p> Signup and view all the answers

What percentage of water is contained in blood plasma?

<p>91.5% (D)</p> Signup and view all the answers

Which of the following fluid types helps bathes all cells, including lymph?

<p>Blood (A)</p> Signup and view all the answers

Which plasma protein is primarily responsible for maintaining osmotic pressure and transporting insoluble molecules?

<p>Albumin (A)</p> Signup and view all the answers

Globulins, a class of plasma proteins, are known for which primary function?

<p>Assisting in immune function (C)</p> Signup and view all the answers

What role does fibrinogen play in blood?

<p>Facilitating blood coagulation (A)</p> Signup and view all the answers

Plasma solutes are composed of electrolytes, nutrients, and respiratory gases, and they account for approximately what percentage of total plasma?

<p>1.5% (B)</p> Signup and view all the answers

If a patient's blood gas analysis reveals an oxygen saturation of 75%, what does it indicate?

<p>Deoxygenation (C)</p> Signup and view all the answers

What is the typical range of sodium (Na+) concentration in blood plasma?

<p>135 - 145 mM (A)</p> Signup and view all the answers

What is the normal range for blood pH?

<p>7.35-7.45 (B)</p> Signup and view all the answers

What is the average range of plasma protein levels considered normal?

<p>60-80 g/L (A)</p> Signup and view all the answers

What is the normal range of potassium (K+) concentration in blood plasma?

<p>3.5-5.5 mM (B)</p> Signup and view all the answers

What is the normal concentration range of calcium (Ca2+) in blood plasma?

<p>2-2.5 mM (D)</p> Signup and view all the answers

What is the normal range of magnesium (Mg2+) concentration in blood plasma?

<p>1-1.5 mM (D)</p> Signup and view all the answers

What is the typical range of chloride (Cl-) concentration in blood plasma?

<p>95-107 mM (B)</p> Signup and view all the answers

What is the normal range of bicarbonate (HCO3-) concentration in blood plasma?

<p>22-26 mM (C)</p> Signup and view all the answers

What is the range of cholesterol concentration typically found in blood plasma?

<p>3.5-5.5 mM (D)</p> Signup and view all the answers

What is the typical concentration range of urea in blood plasma?

<p>2.5-6.5 mM (D)</p> Signup and view all the answers

Given the role of plasma proteins, what might be the consequence of significantly low levels of albumin in the blood?

<p>Edema due to decreased osmotic pressure (B)</p> Signup and view all the answers

Elevated blood urea levels could suggest impairment where?

<p>Kidneys (B)</p> Signup and view all the answers

How does the amount of oxygen saturation affect blood characteristics?

<p>Lower saturation changes blood from bright to dark red. (A)</p> Signup and view all the answers

Individuals with compromised liver function may exhibit reduced levels of which plasma protein?

<p>Albumin (B)</p> Signup and view all the answers

If a blood test reveals a patient's plasma protein levels are consistently above 80 g/L, what could this indicate?

<p>Hyperproteinemia linked to dehydration (A)</p> Signup and view all the answers

Flashcards

Blood viscosity

Blood is more viscous than water.

Venipuncture site

The medial cubital vein is the most common site for venipuncture.

Vacutainer colors

Each vacutainer is color-coded to indicate its contents.

Blood pH range

Blood pH ranges from 7.35 to 7.45.

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Anticoagulant additives

Tubes are often lined with additives to prevent coagulation, determined by the intended tests.

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Blood color and oxygen

Blood color varies with oxygen content; red indicates oxygenated blood, blue indicates deoxygenated blood.

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Heparin

Heparin is an anticoagulant that prevents blood clotting.

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Citrate

Citrate is an additive used in blood collection tubes to prevent coagulation.

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EDTA

EDTA is an anticoagulant commonly used in blood collection tubes.

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Procoagulant tubes

Procoagulant tubes contain substances that promote blood clotting to obtain a clotted sample.

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Blood volume composition

Blood volume accounts for approximately 10% of extracellular fluid and 8% of body mass.

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Total blood volume

Total blood volume is 5-6L in males and 4-5L in females.

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Intracellular Fluid (ICF)

The fluid inside cells, making up about 40% of body weight (28L in a 70kg person).

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Extracellular Fluid (ECF)

Fluid outside cells, approximately 20% of body weight (14L in a 70kg person), including interstitial fluid and plasma.

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Transcellular Fluid

Includes fluids like CSF, urine, and joint fluid, totaling about 0.8L.

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Interstitial Fluid

Fluid that surrounds cells, sharing a similar composition to plasma but with lower protein content.

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Plasma

Liquid component of blood, constituting 55% of total blood volume, contains no cellular components.

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Male Body Water Percentage

Males typically have 60% of their body weight as water, which is about 42L in a 70kg male.

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Female Body Water Percentage

The average female body contains 55% water due to a higher fat content.

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Blood Centrifugation

Blood is centrifuged to separate cellular components from the fluid (plasma).

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Appearance of Plasma

Plasma is a clear, straw-colored liquid.

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Fluid Analysis

Fluid analysis is performed after fluid is removed.

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High Intracellular Ions

Potassium and Magnesium are high inside cells.

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Low Intracellular Ions

Sodium and Chloride are low inside cells.

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Anticoagulant Use

The anticoagulant is used to prevent clotting when drawing blood.

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Plasma Percentage

Plasma is approximately 55% of blood volume.

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Plasma Proteins

Liquid part of blood containing proteins and making up 7% of its total volume.

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Albumin

Plasma proteins that maintain osmotic pressure and transport insoluble molecules.

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Globulins

Plasma proteins that transport ions, hormones, and lipids and assist in immune function.

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Fibrinogen

Plasma protein involved in blood coagulation.

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Plasma Protein Range

Normal level of plasma proteins.

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Plasma Solutes

Electrolytes, nutrients, and respiratory gases.

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Oxygenated Blood Saturation

The percentage of oxygen saturation in oxygenated blood.

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Deoxygenated Blood Saturation

The percentage of oxygen saturation in deoxygenated blood.

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Normal Blood pH

Plasma pH range.

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Study Notes

  • Plasma proteins normally range from 60-80g/L, making up 7% of total plasma

Plasma Proteins

  • Albumin maintains osmotic pressure in blood and transports insoluble molecules
  • Globulins (alpha, beta, and gamma) transport ions, hormones, and lipids and assist in immune function
  • Fibrinogen is a molecular component involved in blood coagulation

Plasma Solutes

  • Plasma is 1.5% total plasma, and contains over 100 solutes including electrolytes, nutrients, and respiratory gases

Cations

  • Sodium (Na+) concentration is 135-145 mM
  • Potassium (K+) concentration is 3.5-5.5 mM
  • Calcium (Ca2+) concentration is 2-2.5 mM
  • Magnesium (Mg2+) concentration is 1-1.5 mM

Anions

  • Chloride (Cl-) concentration is 95-107 mM
  • Bicarbonate (HCO3-) concentration is 22-26 mM
  • Hydrogen Phosphate (HPO42-) and Dihydrogen Phosphate (H2PO4-) concentration is 1.5-2 mM
  • Lactate and Sulfate (SO42-) concentration is ~5 mM

Other Plasma Components

  • pH ranges from 7.35-7.45
  • Glucose concentration is 4.5-5.5 mM
  • Amino acid concentration is ~2 mM
  • Cholesterol concentration is 3.5-5.5 mM
  • Urea concentration is 2.5-6.5 mM
  • Oxygen saturation is 98-99% when oxygenated and 75% when deoxygenated

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