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Biuret Reaction for Protein Determination

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Biuret Reaction for Protein Determination

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Questions and Answers

What is the purpose of Biuret reagent in determining protein presence?

To generate a yellow compound

To produce a colorless compound

To create a green compound

To form a violet compound (correct)

Why is it essential for the Biuret reaction to be carried out in a strongly basic medium?

To increase the reaction speed

To ensure kinetic acceptability (correct)

To enhance the aroma of the reaction

To eliminate color formation

What molecule is responsible for giving positive results in the Biuret reaction?

Urea

Ammonia

Biurea (correct)

Glycine

What type of reactions are those in which the color intensity is proportional to the amount of substance produced?

Colorimetric reactions Signup and view all the answers

How can the concentration of a substance be determined using colorimetric reactions?

By gauging the intensity of color Signup and view all the answers

What is the wavelength range recommended for reading the total protein content?

540 nm - 550 nm Signup and view all the answers

What complex's absorbance is at its maximum around the recommended wavelength range?

Copper-protein complex Signup and view all the answers

Why does the Beer-Lambert Law cease to apply in some cases?

Due to changes in the nature of the absorbent species Signup and view all the answers

What do we prepare to obtain the linear function that relates absorbance to protein concentration?

Standard solution of unflavored gelatin Signup and view all the answers

What is used to adjust the pH for the Biuret reaction?

Sodium hydroxide (NaOH) Signup and view all the answers