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Questions and Answers
What is the purpose of Biuret reagent in determining protein presence?
What is the purpose of Biuret reagent in determining protein presence?
- To generate a yellow compound
- To produce a colorless compound
- To create a green compound
- To form a violet compound (correct)
Why is it essential for the Biuret reaction to be carried out in a strongly basic medium?
Why is it essential for the Biuret reaction to be carried out in a strongly basic medium?
- To increase the reaction speed
- To ensure kinetic acceptability (correct)
- To enhance the aroma of the reaction
- To eliminate color formation
What molecule is responsible for giving positive results in the Biuret reaction?
What molecule is responsible for giving positive results in the Biuret reaction?
- Urea
- Ammonia
- Biurea (correct)
- Glycine
What type of reactions are those in which the color intensity is proportional to the amount of substance produced?
What type of reactions are those in which the color intensity is proportional to the amount of substance produced?
How can the concentration of a substance be determined using colorimetric reactions?
How can the concentration of a substance be determined using colorimetric reactions?
What is the wavelength range recommended for reading the total protein content?
What is the wavelength range recommended for reading the total protein content?
What complex's absorbance is at its maximum around the recommended wavelength range?
What complex's absorbance is at its maximum around the recommended wavelength range?
Why does the Beer-Lambert Law cease to apply in some cases?
Why does the Beer-Lambert Law cease to apply in some cases?
What do we prepare to obtain the linear function that relates absorbance to protein concentration?
What do we prepare to obtain the linear function that relates absorbance to protein concentration?
What is used to adjust the pH for the Biuret reaction?
What is used to adjust the pH for the Biuret reaction?
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