10 Questions
What is the purpose of Biuret reagent in determining protein presence?
To form a violet compound
Why is it essential for the Biuret reaction to be carried out in a strongly basic medium?
To ensure kinetic acceptability
What molecule is responsible for giving positive results in the Biuret reaction?
Biurea
What type of reactions are those in which the color intensity is proportional to the amount of substance produced?
Colorimetric reactions
How can the concentration of a substance be determined using colorimetric reactions?
By gauging the intensity of color
What is the wavelength range recommended for reading the total protein content?
540 nm - 550 nm
What complex's absorbance is at its maximum around the recommended wavelength range?
Copper-protein complex
Why does the Beer-Lambert Law cease to apply in some cases?
Due to changes in the nature of the absorbent species
What do we prepare to obtain the linear function that relates absorbance to protein concentration?
Standard solution of unflavored gelatin
What is used to adjust the pH for the Biuret reaction?
Sodium hydroxide (NaOH)
Learn about the Biuret reaction used to determine the presence of proteins in a mixture. Understand the role of cupric sulfate in alkaline solution and the formation of a coordination complex that leads to a violet compound indicating protein presence.
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