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Questions and Answers
What is the basis of the reaction described in the text?
What is the basis of the reaction described in the text?
- Conversion of peptide bonds to nitrogen
- Conversion of nitrogen in proteins to ammonia (correct)
- Conversion of proteins to peptide bonds
- Conversion of ammonia to nitrogen
Under what conditions does the biuret reaction occur?
Under what conditions does the biuret reaction occur?
- Neutral conditions
- Extreme temperature conditions
- Acidic conditions
- Alkaline conditions (correct)
What color is formed as a result of the biuret reaction?
What color is formed as a result of the biuret reaction?
- Purple (correct)
- Red
- Green
- Yellow
What happens when divalent copper reacts with peptide bonds under alkaline conditions?
What happens when divalent copper reacts with peptide bonds under alkaline conditions?
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Which complex is formed during the biuret reaction according to the text?
Which complex is formed during the biuret reaction according to the text?
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What is the role of glucose oxidase in the reaction described in the text?
What is the role of glucose oxidase in the reaction described in the text?
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In the reaction, what is the function of peroxidase (POD)?
In the reaction, what is the function of peroxidase (POD)?
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What is the purpose of phenol and 4-aminophenazone (4-AP) in this reaction system?
What is the purpose of phenol and 4-aminophenazone (4-AP) in this reaction system?
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What is the relationship between the color intensity and glucose concentration in this assay?
What is the relationship between the color intensity and glucose concentration in this assay?
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Which compound is responsible for detecting the formed hydrogen peroxide in this enzymatic reaction?
Which compound is responsible for detecting the formed hydrogen peroxide in this enzymatic reaction?
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