Biuret Reaction in Assay Principle

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What is the basis of the reaction described in the text?

Conversion of peptide bonds to nitrogen

Conversion of nitrogen in proteins to ammonia (correct)

Conversion of proteins to peptide bonds

Conversion of ammonia to nitrogen

Under what conditions does the biuret reaction occur?

Neutral conditions

Extreme temperature conditions

Acidic conditions

Alkaline conditions (correct)

What color is formed as a result of the biuret reaction?

Purple (correct)

Red

Green

Yellow

What happens when divalent copper reacts with peptide bonds under alkaline conditions?

Formation of purple color Signup and view all the answers

Which complex is formed during the biuret reaction according to the text?

Cu-Protein complex Signup and view all the answers

What is the role of glucose oxidase in the reaction described in the text?

Catalyzes the oxidation of glucose Signup and view all the answers

In the reaction, what is the function of peroxidase (POD)?

Converts hydrogen peroxide to water Signup and view all the answers

What is the purpose of phenol and 4-aminophenazone (4-AP) in this reaction system?

Detect the formed hydrogen peroxide Signup and view all the answers

What is the relationship between the color intensity and glucose concentration in this assay?

The color intensity is directly proportional to glucose concentration Signup and view all the answers

Which compound is responsible for detecting the formed hydrogen peroxide in this enzymatic reaction?

Phenol Signup and view all the answers

Biuret Reaction in Assay Principle

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Questions and Answers

What is the basis of the reaction described in the text?

Under what conditions does the biuret reaction occur?

What color is formed as a result of the biuret reaction?

What happens when divalent copper reacts with peptide bonds under alkaline conditions?

Which complex is formed during the biuret reaction according to the text?

What is the role of glucose oxidase in the reaction described in the text?

In the reaction, what is the function of peroxidase (POD)?

What is the purpose of phenol and 4-aminophenazone (4-AP) in this reaction system?

What is the relationship between the color intensity and glucose concentration in this assay?

Which compound is responsible for detecting the formed hydrogen peroxide in this enzymatic reaction?

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