Podcast
Questions and Answers
A negative Biuret test would result in a purple color change.
A negative Biuret test would result in a purple color change.
False (B)
What is a Biuret Test used for?
What is a Biuret Test used for?
To determine the presence of protein in different substances.
The intensity of the purple color produced in a Biuret test is directly proportional to the protein concentration.
The intensity of the purple color produced in a Biuret test is directly proportional to the protein concentration.
True (A)
What is the principle behind the Biuret test?
What is the principle behind the Biuret test?
Which of the following is NOT a type of sample that can be used for a Biuret test?
Which of the following is NOT a type of sample that can be used for a Biuret test?
What is the primary application of Biuret test results?
What is the primary application of Biuret test results?
What solution is added to the test tube after the sample and distilled water are added?
What solution is added to the test tube after the sample and distilled water are added?
What is the final solution added to the test tube in Biuret test?
What is the final solution added to the test tube in Biuret test?
Flashcards
Biuret Test Principle
Biuret Test Principle
Copper ions react with peptide bonds in proteins, forming a colored complex whose intensity correlates with protein concentration.
Positive Biuret Test
Positive Biuret Test
A purple color indicates the presence of protein in a sample.
Negative Biuret Test
Negative Biuret Test
A blue color indicates no protein is present.
DNA Extraction Principle
DNA Extraction Principle
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DNA Function
DNA Function
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Study Notes
Objective
- Students will be able to test protein presence in substances.
- Students will reflect on two case studies related to collagen defect.
Principle
- Biuret protein assays are based on the principle that copper ions in the reagent react with peptide bonds in proteins.
- The reaction forms a complex that absorbs light at a specific wavelength.
- The intensity of the purple color is directly proportional to the protein concentration in the sample.
- The amount of protein can be quantified by measuring the absorbance.
Negative Biuret Test
- Blue color indicates proteins are absent.
Positive Biuret Test
- Purple color indicates proteins are present.
Biuret Protein Assay
- A simple, sensitive, and reproducible method for determining protein concentration in a sample.
- Widely used in biochemical and biological research
- Can be performed on a wide range of protein samples, including purified proteins, cell lysates, and biological samples.
- Can be used to perform various biochemical and biological studies (enzyme kinetics, protein-protein interactions, and protein expression studies).
Materials
- 6 Test Tubes
- 1 Test Tube Rack
- 2 Test Tube Holders
- 2 250mL Beakers
- 1 10 mL Graduated Pipet
- 1 Pipet Bulb
- 1 Dropper
- 1 Hotplate
- 10% NaOH Solution
- 5% Copper Sulfate Sol'n
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