7 Questions
What is the purpose of using inorganic salt, organic solvent, or heat in protein testing?
To maintain the primary structure of the molecule
Why won't free amino acids give a positive result with the biuret test?
They have no peptide bonds
How many amino acids bonded together by peptide bonds are needed to respond positively to the biuret test?
4
After heating albumin at high temperature, is it still biologically active?
Yes, it remains biologically active
Can the salting out method be used in fractionating mixtures of proteins?
Yes, this is a common method for fractionation
Which chemical process is associated with cleavage of primary chemical bonds in proteins?
Protein denaturation
What effect does boiling water have on the structure of proteins?
Weakens hydrogen bonds
Learn about qualitative tests of proteins, focusing on the biuret test. This test is specific for the peptide bond and is used to detect the presence of peptides or proteins containing at least two peptide linkages. Understand how certain functional groups in proteins produce characteristically colored products.
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