Qualitative Protein Tests: Biuret Test
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Questions and Answers

What is the purpose of using inorganic salt, organic solvent, or heat in protein testing?

  • To maintain the primary structure of the molecule (correct)
  • To enhance the color development in the test
  • To break down the primary chemical bonds
  • To alter the primary structure of the molecule

    • Why won't free amino acids give a positive result with the biuret test?

  • They lack the necessary chemical elements
  • They do not react with inorganic salts
  • They degrade under heat
  • They have no peptide bonds (correct)

    • How many amino acids bonded together by peptide bonds are needed to respond positively to the biuret test?

  • 3
  • 4 (correct)
  • 1
  • 2

    • After heating albumin at high temperature, is it still biologically active?

    <p>Yes, it remains biologically active</p> Signup and view all the answers

    Can the salting out method be used in fractionating mixtures of proteins?

    <p>Yes, this is a common method for fractionation</p> Signup and view all the answers

    Which chemical process is associated with cleavage of primary chemical bonds in proteins?

    <p>Protein denaturation</p> Signup and view all the answers

    What effect does boiling water have on the structure of proteins?

    <p>Weakens hydrogen bonds</p> Signup and view all the answers

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