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Questions and Answers
What should be recorded every 3 minutes during the hypoxic hypoxia procedure?
What should be recorded every 3 minutes during the hypoxic hypoxia procedure?
How is sodium nitrite administered to the mice in the hemic hypoxia method?
How is sodium nitrite administered to the mice in the hemic hypoxia method?
What is the primary observation made after the death of the mouse in the hypoxic hypoxia experiment?
What is the primary observation made after the death of the mouse in the hypoxic hypoxia experiment?
What should be done immediately after administering sodium nitrite to one of the mice?
What should be done immediately after administering sodium nitrite to one of the mice?
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What is the purpose of using sodium lime in the hypoxic hypoxia method?
What is the purpose of using sodium lime in the hypoxic hypoxia method?
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Study Notes
Methods and Procedures
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Hypoxic Hypoxia:
- Administer a small amount of sodium lime (5g) to a mouse in a sealed bottle.
- Observe and record the mouse's general condition, respiratory rate (per 10 seconds), depth, skin and mucous membrane color.
- Seal the bottle tightly and monitor every 3 minutes until the mouse dies.
- Record the survival duration.
- Open the mouse's abdomen to examine the color of the blood and liver.
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Hemic Hypoxia
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CO Poisoning:
- Place a mouse in a bottle.
- Observe and record general condition, respiratory rate, depth, and skin/mucosa color.
- Introduce 5ml of Carbon Monoxide (CO) into the bottle.
- Seal the bottle immediately.
- Begin recording the time and observe until the mouse dies.
- Document the survival duration.
- Open the abdomen of the deceased mouse to examine the color of the blood and liver.
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Sodium Nitrite Poisoning:
- Observe two mice for normal indicators.
- Inject one mouse with 0.3 ml sodium nitrite intraperitoneally (IP) to avoid liver damage; inject the other with saline water IP.
- Immediately inject methylene blue (0.3 ml) into one mouse, and saline to the other.
- Record the survival time.
- Open the abdomen to examine the color of the blood and liver.
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Control Group:
- Observe a healthy mouse.
- Kill the mouse by cervical dislocation.
- Open the abdomen to examine the color of the blood and liver.
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Description
Explore the methods and procedures for studying hypoxic hypoxia and hemic hypoxia using mice. This quiz examines the administration of substances and observation techniques related to respiratory conditions and survival analysis. Gain insights into the implications of carbon monoxide and sodium nitrite poisoning on the physiological responses of subjects.
