Podcast
Questions and Answers
What should be recorded every 3 minutes during the hypoxic hypoxia procedure?
What should be recorded every 3 minutes during the hypoxic hypoxia procedure?
- The heart rate of the mouse
- The mouse's respiratory frequency and depth (correct)
- The amount of sodium lime used
- The temperature of the environment
How is sodium nitrite administered to the mice in the hemic hypoxia method?
How is sodium nitrite administered to the mice in the hemic hypoxia method?
- Intravenously
- Orally
- Intranasally
- Intraperitoneally (correct)
What is the primary observation made after the death of the mouse in the hypoxic hypoxia experiment?
What is the primary observation made after the death of the mouse in the hypoxic hypoxia experiment?
- Weight of the mouse
- Color of the blood and liver (correct)
- Presence of disease symptoms
- Condition of the lungs
What should be done immediately after administering sodium nitrite to one of the mice?
What should be done immediately after administering sodium nitrite to one of the mice?
What is the purpose of using sodium lime in the hypoxic hypoxia method?
What is the purpose of using sodium lime in the hypoxic hypoxia method?
Flashcards
Hypoxic Hypoxia
Hypoxic Hypoxia
A type of hypoxia where the body's tissues are starved of oxygen due to a lack of oxygen in the air being breathed (e.g., high altitude).
Hemic Hypoxia
Hemic Hypoxia
A type of hypoxia where the blood's ability to carry oxygen is reduced due to inadequate red blood cell production (e.g., anemia) or carbon monoxide poisoning.
CO Poisoning
CO Poisoning
A type of hemic hypoxia caused by carbon monoxide (CO) binding to hemoglobin, preventing oxygen transport.
Sodium Nitrite Poisoning
Sodium Nitrite Poisoning
Signup and view all the flashcards
Methylene Blue
Methylene Blue
Signup and view all the flashcards
Study Notes
Methods and Procedures
-
Hypoxic Hypoxia:
- Administer a small amount of sodium lime (5g) to a mouse in a sealed bottle.
- Observe and record the mouse's general condition, respiratory rate (per 10 seconds), depth, skin and mucous membrane color.
- Seal the bottle tightly and monitor every 3 minutes until the mouse dies.
- Record the survival duration.
- Open the mouse's abdomen to examine the color of the blood and liver.
-
Hemic Hypoxia
-
CO Poisoning:
- Place a mouse in a bottle.
- Observe and record general condition, respiratory rate, depth, and skin/mucosa color.
- Introduce 5ml of Carbon Monoxide (CO) into the bottle.
- Seal the bottle immediately.
- Begin recording the time and observe until the mouse dies.
- Document the survival duration.
- Open the abdomen of the deceased mouse to examine the color of the blood and liver.
-
Sodium Nitrite Poisoning:
- Observe two mice for normal indicators.
- Inject one mouse with 0.3 ml sodium nitrite intraperitoneally (IP) to avoid liver damage; inject the other with saline water IP.
- Immediately inject methylene blue (0.3 ml) into one mouse, and saline to the other.
- Record the survival time.
- Open the abdomen to examine the color of the blood and liver.
-
-
Control Group:
- Observe a healthy mouse.
- Kill the mouse by cervical dislocation.
- Open the abdomen to examine the color of the blood and liver.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
