Methods and Procedures PDF

## Methods and Procedures 1. **Hypoxic hypoxia:** Take a little sodium lime (5 g) and a mouse into a bottle. Observe and record the general condition, respiratory frequency (/10s) and depth, as well as skin and mucosa color. Then plug the bottle stopper tightly and begin to record the time. Repeat...

## Methods and Procedures 1. **Hypoxic hypoxia:** Take a little sodium lime (5 g) and a mouse into a bottle. Observe and record the general condition, respiratory frequency (/10s) and depth, as well as skin and mucosa color. Then plug the bottle stopper tightly and begin to record the time. Repeat the observation at every 3 minutes interval until the death of the mouse. Record the survival duration. Open the abdomen of the mouse corpse to observe the color of blood and liver. 2. **Hemic hypoxia** * **CO poisoning:** Take a mouse into a bottle. Observe and record the general condition, respiratory frequency and depth, as well as skin and mucosa color. Turn on the switch and take 5 ml CO from the tube into the bottle. Then plug the bottle stopper quickly and begin to record the time until the death of the mouse. Record the survival duration. Open the abdomen of the mouse corpse to observe the color of blood and liver. * **Sodium nitrite poisoning:** Take 2 mice and observe their normal indexes. Then give each mouse sodium nitrite 0.3 ml by ip (intraperitoneal injection) respectively. (In order to avoid hepatic injury in the mouse, the ip site should move slightly to the left inferior abdomen.) Immediately followed by ip of methylene blue 0.3 ml to one mouse, and saline water to the other. Record the survival duration. Open the abdomen of the mouse corpse to observe the color of blood and liver. 3. **Take a normal mouse and observe the normal condition. Then kill the mouse by cervical dislocation. Open the abdomen of the mouse corpse to observe the color of blood and liver.**