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Questions and Answers
What is the purpose of recording the respiratory frequency and depth of the mouse during the hypoxic hypoxia procedure?
What is the purpose of recording the respiratory frequency and depth of the mouse during the hypoxic hypoxia procedure?
- To determine the mouse's feeding behavior
- To assess the mouse's reaction to environmental changes
- To monitor the effects of hypoxia on breathing (correct)
- To evaluate the impact of anesthesia
In the case of hemic hypoxia due to CO poisoning, what should be observed as an indicator of the mouse's condition?
In the case of hemic hypoxia due to CO poisoning, what should be observed as an indicator of the mouse's condition?
- Weight loss over time
- Color of the blood and liver after death (correct)
- Increase in activity levels
- Change in body temperature
When conducting the sodium nitrite poisoning experiment, what is the purpose of administering methylene blue to one of the mice?
When conducting the sodium nitrite poisoning experiment, what is the purpose of administering methylene blue to one of the mice?
- To neutralize the effects of sodium nitrite (correct)
- To enhance the toxicity of sodium nitrite
- To speed up the death process
- To observe its effect on weight gain
What is indicated by the color of the blood and liver observed after killing a normal mouse?
What is indicated by the color of the blood and liver observed after killing a normal mouse?
During the hypoxic hypoxia experiment, what crucial factor must be recorded in addition to the general condition of the mouse?
During the hypoxic hypoxia experiment, what crucial factor must be recorded in addition to the general condition of the mouse?
Flashcards
Hypoxic Hypoxia
Hypoxic Hypoxia
The condition where there is insufficient oxygen in the tissues due to reduced oxygen levels in the blood.
Hemic Hypoxia
Hemic Hypoxia
The condition where there is insufficient oxygen in the tissues due to the blood's inability to carry enough oxygen.
CO Poisoning
CO Poisoning
A type of hemic hypoxia caused by carbon monoxide poisoning.
Sodium Nitrite Poisoning
Sodium Nitrite Poisoning
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Cervical Dislocation
Cervical Dislocation
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Study Notes
Methods and Procedures
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Hypoxic Hypoxia: Introduce a mouse and 5g sodium lime into a sealed bottle. Observe and record the general condition, respiratory rate (per 10 seconds), depth, and skin/mucosa colour, every 3 minutes until death. Note survival time. Open the abdomen and examine blood/liver colour.
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Hemic Hypoxia
- CO Poisoning: Place a mouse in a bottle. Observe and record general condition, respiratory rate, depth, skin/mucosa colour. Introduce 5ml CO into the bottle, immediately seal and begin timing until death. Note survival time. Open abdomen and examine blood/liver colour.
- Sodium Nitrite Poisoning: Observe normal indices of two mice. Inject one with 0.3ml sodium nitrite (intraperitoneally, slightly to the left inferior abdomen) and the other with 0.3ml methylene blue (intraperitoneally). Immediately inject saline to one mouse. Note survival time. Open abdomen to examine blood/liver colour.
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Normal Condition Control: A normal mouse is observed and sacrificed via cervical dislocation. The abdomen is opened to observe blood/liver colour as a control.
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