Biology Experiment on Hypoxia in Mice

What is the purpose of recording the respiratory frequency and depth of the mouse during the hypoxic hypoxia procedure?

In the case of hemic hypoxia due to CO poisoning, what should be observed as an indicator of the mouse's condition?

When conducting the sodium nitrite poisoning experiment, what is the purpose of administering methylene blue to one of the mice?

What is indicated by the color of the blood and liver observed after killing a normal mouse?

During the hypoxic hypoxia experiment, what crucial factor must be recorded in addition to the general condition of the mouse?

Methods and Procedures

Hypoxic Hypoxia: Introduce a mouse and 5g sodium lime into a sealed bottle. Observe and record the general condition, respiratory rate (per 10 seconds), depth, and skin/mucosa colour, every 3 minutes until death. Note survival time. Open the abdomen and examine blood/liver colour.
Hemic Hypoxia
- CO Poisoning: Place a mouse in a bottle. Observe and record general condition, respiratory rate, depth, skin/mucosa colour. Introduce 5ml CO into the bottle, immediately seal and begin timing until death. Note survival time. Open abdomen and examine blood/liver colour.
- Sodium Nitrite Poisoning: Observe normal indices of two mice. Inject one with 0.3ml sodium nitrite (intraperitoneally, slightly to the left inferior abdomen) and the other with 0.3ml methylene blue (intraperitoneally). Immediately inject saline to one mouse. Note survival time. Open abdomen to examine blood/liver colour.
Normal Condition Control: A normal mouse is observed and sacrificed via cervical dislocation. The abdomen is opened to observe blood/liver colour as a control.

This quiz covers the methods and procedures for studying hypoxic conditions in mice, including hypoxic hypoxia and hemic hypoxia cases. It focuses on experimental observations, survival times, and physiological responses under different poisoning scenarios. Test your knowledge about these biological experiments and their outcomes.