Biology Exam 1: Chapters 1-7

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Questions and Answers

What does the presence of 54 colonies on a plate indicate about the original milk sample?

  • 54 cells
  • 54,000 cells (correct)
  • 540 cells
  • 5,400 cells

Which bacteria are primarily found in the intestines of humans?

  • Mycoplasma
  • Facultatively anaerobic gram-negative rods (correct)
  • Staphylococcus
  • Chlamydias

What is the main reproductive method of most bacteria?

  • Meiosis
  • Mitosis
  • Binary fission (correct)
  • Budding

Which method is considered the best for sterilizing heat-labile solutions?

<p>Membrane filtration (D)</p> Signup and view all the answers

Which group includes multicellular heterotrophs with chitin cell walls?

<p>Fungi (A)</p> Signup and view all the answers

What type of medium allows only gram-positive organisms and Staphylococcus aureus to grow?

<p>Selective and differential medium (D)</p> Signup and view all the answers

Which of the following is NOT considered an enteric organism?

<p>Campylobacter (A)</p> Signup and view all the answers

What is an example of a method that transfers DNA horizontally to cells in the same generation?

<p>Conjugation (D)</p> Signup and view all the answers

What is true about the pattern of microbial death?

<p>Cells die at a constant rate (C)</p> Signup and view all the answers

What characteristic differentiates Rickettsias from Chlamydias?

<p>Rickettsias require an arthropod for transmission (C)</p> Signup and view all the answers

Which type of mutation is likely the cause of prostate cancer, based on common genetic mutations?

<p>Nonsense (D)</p> Signup and view all the answers

Which type of bacteria is known for causing nosocomial infections?

<p>Pseudomonas aeruginosa (A)</p> Signup and view all the answers

Which of the following statements about antimicrobial control agents is FALSE?

<p>They are always effective against all bacterial types (A)</p> Signup and view all the answers

How many cells would be present after 5 hours if three cells with a generation time of 60 minutes are inoculated?

<p>96 cells (D)</p> Signup and view all the answers

What structure is introduced into a cell to assist in gene transmission from one organism to another?

<p>Vector (C)</p> Signup and view all the answers

Which statement regarding fungi is FALSE?

<p>Most fungi are pathogenic for humans (C)</p> Signup and view all the answers

What term describes an agent that removes pathogenic bacteria from a medical instrument?

<p>Disinfectant (D)</p> Signup and view all the answers

Which of the following statements about protozoa is FALSE?

<p>Nearly all protozoa cause disease (C)</p> Signup and view all the answers

In the lac operon model, which component binds to the repressor for transcription to occur?

<p>Lactose (B)</p> Signup and view all the answers

Which organism is responsible for causing ringworm?

<p>Fungus (A)</p> Signup and view all the answers

Which of the following is a characteristic feature of a clone?

<p>Genetically identical cells from a single cell (C)</p> Signup and view all the answers

Which evidence supports the three-domain system of classification?

<p>Ribosomal RNA sequence comparisons (C)</p> Signup and view all the answers

Which statement best describes the definition of a strain in microbiology?

<p>A pure culture that is not totally identical to other cultures of the same species (B)</p> Signup and view all the answers

What type of cells does phage typing target?

<p>Specific bacterial species (B)</p> Signup and view all the answers

What is the significance of Antoni van Leeuwenhoek in microbiology?

<p>He was the first to view protozoa and bacteria. (A)</p> Signup and view all the answers

Which statement is NOT a characteristic of fungi?

<p>Fungi are autotrophic organisms. (D)</p> Signup and view all the answers

Which statement about Koch’s postulates is inaccurate?

<p>A suspected pathogen must be found in the majority of individuals with a particular disease. (A)</p> Signup and view all the answers

What is Lipid A commonly known as?

<p>Endotoxin (A)</p> Signup and view all the answers

Which of the following statements about endospores is true?

<p>Endospores can survive extreme conditions. (D)</p> Signup and view all the answers

What type of flagella do spiral bacteria, which cause syphilis and Lyme disease, possess?

<p>Axial filaments (A)</p> Signup and view all the answers

Which of the following cells do NOT function as antigen-presenting cells (APCs)?

<p>Natural killer cells (C)</p> Signup and view all the answers

Which type of microscope is used to observe specimens that emit light when illuminated with ultraviolet light?

<p>Fluorescence microscope (A)</p> Signup and view all the answers

What is the most common class of antibodies found in serum?

<p>IgG (A)</p> Signup and view all the answers

Which type of vaccine is characterized by the use of a weakened form of the disease-causing agent?

<p>Attenuated live-agent vaccine (D)</p> Signup and view all the answers

Which of the following is NOT true regarding phospholipids?

<p>They are primarily used for energy storage. (D)</p> Signup and view all the answers

What event follows the recognition of an antigen by a TH cell?

<p>Digestion of the antigen and presentation with MHC II (B)</p> Signup and view all the answers

In which of the following scenarios would you expect the presence of IgA antibodies?

<p>Signifying a current infection (C)</p> Signup and view all the answers

What test is commonly used for identifying Streptococcus pyogenes from a throat swab?

<p>Direct fluorescent-antibody test (D)</p> Signup and view all the answers

What term describes the interaction between an antibody and soluble antigens resulting in lattice formation?

<p>Precipitation reaction (B)</p> Signup and view all the answers

What occurs during the hemagglutination inhibition test when clumping of test cells is observed?

<p>Negative result for the presence of virus (D)</p> Signup and view all the answers

Which test is the most effective for detecting the presence of HIV antibodies?

<p>Indirect ELISA (A)</p> Signup and view all the answers

Which statement regarding measles is true?

<p>The disease is highly contagious. (C)</p> Signup and view all the answers

What may result from systemic anaphylaxis?

<p>Shock (D)</p> Signup and view all the answers

Which antibodies would a person with blood type B, Rh+ have in their serum?

<p>Anti-A (C)</p> Signup and view all the answers

What is the primary mechanism of HIV transmission worldwide?

<p>Heterosexual intercourse (D)</p> Signup and view all the answers

Which outcome may result from an HIV infection?

<p>Development of AIDS (A)</p> Signup and view all the answers

Why are protozoan and helminthic diseases challenging to treat?

<p>Their cells are structurally and functionally similar to human cells. (C)</p> Signup and view all the answers

Which antimicrobial agent is recommended for treating fungal infections?

<p>Amphotericin B (B)</p> Signup and view all the answers

Flashcards

Bacterial Colony Formation

A colony is a visible cluster of bacteria arising from the growth of a single original bacterial cell.

Hypertonic Pressure Preservation

Using high salt or sugar concentrations to prevent microbial growth by creating a hypertonic environment.

Binary Fission

A method of bacterial reproduction where a single cell divides into two identical daughter cells.

Selective and Differential Media

Culture media designed to selectively grow certain types of bacteria and allow differentiation between different types.

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Bacterial Growth Curve

A graph depicting the growth pattern of bacteria over time, showing phases like lag, log, stationary, and death.

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Microbial Growth Measurement

Methods to track microbial growth, like measuring nutrient consumption (e.g., glucose).

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Bacterial Generation Time

The time it takes for a bacterial population to double in size.

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Disinfectant

An agent that kills or inactivates pathogenic bacteria on inanimate objects.

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Antoni van Leeuwenhoek's discovery

He was the first to observe bacteria and protozoa.

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Fungi characteristic (not)

Fungi have cell walls, unlike some other organisms.

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Algae function (not)

Algae don't play a major role in breaking down dead matter.

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Pasteur's Contribution

Pasteur proved the theory of biogenesis (living from living).

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Koch's Postulates (inaccurate part)

Not all individuals with a disease will display the same infection.

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Genetic Engineering Application

Producing a human protein in bacteria is an example of genetic engineering.

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Bacterial cell wall component (not)

Teichoic acids are a component of some bacterial cell walls.

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Endospore function (not)

Endospores are not for reproduction but for survival under harsh conditions.

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Transformation (Genetics)

Transfer of DNA from one cell to another without cell-to-cell contact.

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Inducible enzyme synthesis (Operon Model)

Enzyme production triggered by a substrate binding to the repressor protein.

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Conjugation (Genetics)

Transfer of DNA between cells of the same generation.

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Biotechnology (definition)

Using living organisms or their products for practical application.

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Vector (Genetics)

Self-replicating DNA used for gene transfer between organisms.

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Three-Domain system (evidence)

Based on comparison of ribosomal RNA sequences.

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Virus Classification (reason for separate domain)

Viruses are not composed of cells, unlike the three domains.

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Clone (definition)

Genetically identical cells from a single-cell.

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Facultatively anaerobic gram-negative rods

Bacteria that can survive with or without oxygen and stain gram-negative, found primarily in the intestines.

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Staphylococcus and Streptococcus differentiation

Differentiated by their growth in high salt concentrations. Staphylococcus grows better in high salt.

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Multicellular heterotroph with chitin cell walls

A fungus; an organism that gets nutrients from other organisms and has chitin cell walls.

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Non-enteric bacteria

A bacteria that is not found in the intestines. Example Campylobacter.

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Bacteria lacking a cell wall

Mycoplasma; a type of bacteria that doesn't have a cell wall, unlike most other bacteria.

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Gram-negative rod fermenting lactose

Citrobacter; a gram-negative rod that ferments lactose and uses citric acid as its sole carbon source.

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Bacteria requiring an arthropod for transmission

Rickettsias are bacteria needing an arthropod, like a tick or mosquito, to spread to humans.

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Haemophilus identification

Haemophilus bacteria are identified by their need for X and V factors.

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Artificially acquired active immunity

Immunity developed through intentional exposure to a weakened or inactive form of a pathogen, stimulating the immune system to produce antibodies.

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What cell is NOT an antigen presenting cell?

Natural killer cells are cytotoxic lymphocytes that directly kill infected cells, but don't present antigens to other immune cells.

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Antibody binding to a toxin

Neutralization is when an antibody binds to a toxin, preventing it from binding to its target cell and causing harm.

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What recognizes antigens on MHC II?

Helper T cells (Th cells) recognize antigens presented on MHC II molecules by antigen-presenting cells, activating the immune response.

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B cell characteristic?

B cells do not directly recognize antigens associated with MHC I, which is the primary mechanism for recognizing viral or intracellular pathogens.

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Most abundant antibody in serum

IgG is the most common type of antibody found in blood serum, providing long-term immunity against various pathogens.

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What is an antigen?

An antigen is a substance that triggers an immune response by stimulating the production of antibodies that specifically bind to it.

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Third step of antibody response

After antigen recognition, the third step involves a helper T cell (Th cell) recognizing the processed antigen presented on MHC II by an antigen-presenting cell (APC).

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Indirect ELISA

A laboratory test used to detect the presence of antibodies against a specific antigen, like HIV antibodies.

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Measles Misconception

The statement that measles has been eradicated in the United States is false. While vaccination has significantly reduced cases, measles outbreaks still occur.

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Hypersensitivity: First Exposure?

Hypersensitivity reactions occur when the immune system overreacts to a harmless substance, but this reaction doesn't happen on the first exposure to the allergen.

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Anaphylaxis: Systemic Effect

Systemic anaphylaxis is a severe allergic reaction affecting the entire body, potentially leading to shock.

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Blood Type B, Rh+ Antibodies

A person with blood type B, Rh+ will have anti-A antibodies in their serum.

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Graft-versus-Host Disease

Graft-versus-host disease is a complication primarily seen after skin grafts or bone marrow transplants, where the donor cells attack the recipient's tissues.

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Allergic Contact Dermatitis: Cause

Allergic contact dermatitis is triggered by sensitized T cells reacting to allergens, which can lead to inflammation and skin irritation.

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HIV Attachment: Gp120 and CD4+

HIV attaches to the target cells through the interaction of the viral Gp120 protein with the CD4+ receptor.

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Study Notes

Exam 1, Chapters 1-7

  • Antoni van Leeuwenhoek was the first person to view protozoa and bacteria.
  • Fungi lack cell walls.
  • Algae play a role in degrading dead plants and animals.
  • Pasteur's work proved that living cells only come from other living organisms.
  • Koch's postulates are inaccurate in stating that a suspected pathogen must be found in all individuals with the disease.
  • Genetic engineering is used to produce human blood-clotting protein in E. coli.
  • Algae inhabit aquatic and marine environments.
  • Phospholipids are not associated with amino acids.
  • Cilia are short, hair-like structures found on eukaryotic cells for movement.
  • Teichoic acids are not associated with bacterial cell walls.
  • Peptidoglycan is a mesh of peptides and polysaccharides that make up bacterial cell walls.
  • Lipid A is also known as endotoxin.
  • Lipid-soluble molecules cross cell membranes by diffusion.
  • A hypotonic solution causes water to move into the cell.
  • Endosymbiotic theory: the folded inner membrane of mitochondria evolved from the plasma membrane of prokaryotes.
  • Endospores are not true of mitochondria.

Exam 2, Chapters 8, 10-12

  • A gene is a sequence of nucleotides that codes for a functional protein.
  • DNA strands run antiparallel.
  • Transformation is the transfer of naked DNA in solution.
  • Streptomycin-resistant bacteria are colonies 4 and 8.
  • Enzyme A's compound C binds to the repressor.
  • Conjugation transfers DNA horizontally in the same generation.
  • Biotechnology uses microorganisms/animal cells and creates disease-resistant crops.
  • cDNA is used for easier gene transfer.
  • A genus is a taxon of one or more species.
  • The three-domain system classifies organisms based on ribosomal RNA.
  • Viruses are not part of the three domains.
  • Prokaryotic cells first existed 3.5 billion years ago.
  • A pure culture is identical to other cultures of the same species.

Exam 4, Chapters 17-20

  • Vaccination results in artificially acquired active immunity.
  • Natural killer cells are not antigen-presenting cells (APCs).
  • Antibodies binding to toxins results in neutralization.
  • Th cells recognize antigens displayed on cells with MHC II.
  • The most abundant antibody class in serum is IgG.
  • An antigen is a chemical eliciting an antibody response.
  • The third step in eliciting an antibody response involves TH cells recognizing antigen-digest and MHC II.
  • Finding IgA present indicates a current infection.
  • Perforin creates transmembrane channels in cells.
  • Thymic selection destroys T cells that do not recognize self-MHC molecules.
  • Hemagglutination-inhibition tests are used to identify antibodies against a virus.
  • ELISA is a test to detect antibodies.
  • Precipitation reactions occur when antibodies form lattices with antigens.

Additional Exam Information (Multiple Pages)

  • Disinfectant: removes harmful pathogens from medical instruments.
  • A true antimicrobial is equally effective against bacteria and viruses.
  • Thermal death time is the time needed to kill all microbes at a certain temperature.
  • Gram-negative bacteria are the most resistant to chemical biocides.
  • Different types of antimicrobial agents have different uses based on the pathogens they target.
  • Bacterial viruses only attack a specific species.
  • Clones are genetically identical cells from a single cell.
  • Two organisms are closely related if their genomic DNA can hybridize.
  • Facultative anaerobic gram-negative rods are in the intestines of humans.
  • Fungi have chitin cell walls.
  • Mycoplasma lacks a cell wall.
  • Rickettsias need an arthropod for transmission.
  • Haemophilus doesn't belong with the groups in that particular question.
  • Staphylococcus belongs with other gram-positive bacteria.
  • Pseudomonas is known for nosocomial infections.
  • Most fungi are not pathogenic.
  • Ringworm is a fungal infection.
  • Helminthic diseases are typically transmitted through the gastrointestinal system.
  • Mosquitoes are the definitive hosts for malaria.
  • Humans are definitive hosts in many cases; crayfish are intermediate.
  • Algae are not always unicellular.
  • Giardia and Trichomonas lack mitochondria.
  • Ringworm is caused by a fungus.
  • Amphotericin B is used against fungi.
  • Most antibiotics are produced by bacteria.
  • Interferons don't inhibit glycolysis; bacterial cells have similar structures and functions to human cells as a difficulty in treating protozoa and helminths.
  • Drugs that competitively inhibit DNA polymerase are effective against helminths.
  • Tamiflu is an influenza drug that inhibits a viral enzyme.
  • Antibiotics selecting for resistant bacteria can lead to antibiotic resistance in animals and humans.

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