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Questions and Answers
What is the structure and function of DNA in eukaryotic cells?
What is the structure and function of DNA in eukaryotic cells?
DNA is a double-stranded molecule located in the nucleus that contains the genetic information necessary for producing proteins.
How do haploid and diploid cells differ in terms of chromosome number?
How do haploid and diploid cells differ in terms of chromosome number?
Haploid cells contain one complete set of chromosomes (23 in humans), while diploid cells contain two sets (46 in humans).
What are chromosomes and what role do they play in genetics?
What are chromosomes and what role do they play in genetics?
Chromosomes are structures made of DNA and protein that organize genetic material, with humans having 46 chromosomes divided into 23 pairs.
What is the significance of the gene's role in inheritance?
What is the significance of the gene's role in inheritance?
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Why is cell division important for multicellular organisms?
Why is cell division important for multicellular organisms?
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What are two benefits of genetic modification in crops?
What are two benefits of genetic modification in crops?
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Describe the initial process used in cloning Dolly the sheep.
Describe the initial process used in cloning Dolly the sheep.
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Why is cloning adult somatic cells more challenging than embryonic cells?
Why is cloning adult somatic cells more challenging than embryonic cells?
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What happens to the embryo after confirming normal development during Dolly's cloning process?
What happens to the embryo after confirming normal development during Dolly's cloning process?
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What is differentiation in the context of cell biology?
What is differentiation in the context of cell biology?
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List one ethical issue associated with the use of in vitro fertilization.
List one ethical issue associated with the use of in vitro fertilization.
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How can biotechnology contribute to food security?
How can biotechnology contribute to food security?
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What are the implications of epigenetic modifications in the cloning process?
What are the implications of epigenetic modifications in the cloning process?
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What are sister chromatids and how do they differ from homologous chromosomes?
What are sister chromatids and how do they differ from homologous chromosomes?
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What is a tetrad and when does it form during meiosis?
What is a tetrad and when does it form during meiosis?
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Describe the process of crossing over and its significance in genetic diversity.
Describe the process of crossing over and its significance in genetic diversity.
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What role do spindle fibers play during cell division?
What role do spindle fibers play during cell division?
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How does spermatogenesis differ from oogenesis in terms of product and process?
How does spermatogenesis differ from oogenesis in terms of product and process?
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What defines menopause and at what age does it typically occur?
What defines menopause and at what age does it typically occur?
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What are autosomes, and how do they differ from sex chromosomes?
What are autosomes, and how do they differ from sex chromosomes?
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Explain the concept of segregation in meiosis and its importance.
Explain the concept of segregation in meiosis and its importance.
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What are alleles and how do they relate to traits in an organism?
What are alleles and how do they relate to traits in an organism?
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Explain Mendel’s Law of Segregation.
Explain Mendel’s Law of Segregation.
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What does the term ‘homozygous’ mean in genetics?
What does the term ‘homozygous’ mean in genetics?
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Differentiate between genotype and phenotype.
Differentiate between genotype and phenotype.
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What is a Punnett square and its purpose in genetics?
What is a Punnett square and its purpose in genetics?
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What do shaded and unshaded shapes represent in a pedigree?
What do shaded and unshaded shapes represent in a pedigree?
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Describe Mendel’s Law of Independent Assortment.
Describe Mendel’s Law of Independent Assortment.
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What does 'F1' signify in genetics?
What does 'F1' signify in genetics?
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What is nondisjunction and what are its effects during meiosis?
What is nondisjunction and what are its effects during meiosis?
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Define aneuploidy and give two examples.
Define aneuploidy and give two examples.
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Explain the role of maternal age in meiotic nondisjunction.
Explain the role of maternal age in meiotic nondisjunction.
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What is Turner syndrome and what are its characteristics?
What is Turner syndrome and what are its characteristics?
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Describe Klinefelter syndrome and its possible effects on individuals.
Describe Klinefelter syndrome and its possible effects on individuals.
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What is the significance of a karyotype in genetics?
What is the significance of a karyotype in genetics?
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How does polyploidy differ from nondisjunction, and in which organisms is it common?
How does polyploidy differ from nondisjunction, and in which organisms is it common?
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What are the effects of nondisjunction during mitosis compared to meiosis?
What are the effects of nondisjunction during mitosis compared to meiosis?
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What is the role of the Barr body in female mammals?
What is the role of the Barr body in female mammals?
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Why are fruit flies commonly used in genetic experiments?
Why are fruit flies commonly used in genetic experiments?
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How does artificial insemination differ from in vitro fertilization?
How does artificial insemination differ from in vitro fertilization?
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What defines recombinant DNA, and can you provide an example?
What defines recombinant DNA, and can you provide an example?
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Why is plant cloning generally easier than animal cloning?
Why is plant cloning generally easier than animal cloning?
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What are two reasons for using biotechnology in agriculture?
What are two reasons for using biotechnology in agriculture?
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What is gene therapy and its purpose?
What is gene therapy and its purpose?
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Why might the success rate of animal cloning be lower than that of plant cloning?
Why might the success rate of animal cloning be lower than that of plant cloning?
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Study Notes
DNA
- Located in the nucleus of eukaryotes
- Contains genetic information
- Double-stranded and twisted into a double helix
Gene
- Short section of DNA
- Used to produce proteins
- Determines traits
Chromosomes
- Structures organizing genetic material
- Made of DNA and protein
- One unbroken DNA molecule forms each chromosome
- Humans have 46 chromosomes (23 pairs)
- 22 pairs are autosomes, 1 pair are sex chromosomes (e.g., X and Y)
- Females are XX, males are XY
Haploid vs. Diploid
Haploid (n)
- Contains one complete set of chromosomes (n)
- In humans, haploid cells have 23 chromosomes (one from each pair)
- Found in gametes (sperm and egg cells)
- Formed through meiosis, reducing the chromosome number by half
Diploid (2n)
- Contains two complete sets of chromosomes (2n)
- In humans, diploid cells have 46 chromosomes (23 pairs)
- One chromosome of each pair is inherited from each parent
- Found in most body cells (somatic cells)
- Formed through mitosis
Meiosis
- Process forming gametes (sex cells)
- Involves two stages of cell division resulting in cells with half the number of chromosomes
Meiosis 1
- Prophase 1: Nuclear membrane dissolves, chromosomes condense, homologous chromosomes come together (synapse) to form a tetrad, crossing over occurs
- Metaphase 1: Chromosome pairs line up side by side at the equatorial plate
- Anaphase 1: Each chromosome separates from its homologue (pair), moving to opposite poles, the chromosomes do not separate at the centromere
- Telophase 1: Nucleus reforms around chromosomes, cytoplasm divides (cytokinesis occurs).
- Resulting in two haploid daughter cells.
Meiosis 2
- Very similar to mitosis (but with half the number of chromosomes)
- Prophase 2: Nuclear membrane dissolves
- Metaphase 2: Chromosomes line up along equatorial plate
- Anaphase 2: Sister chromatids are separated and move to opposite poles
- Telophase 2: Nucleus reforms, Cytoplasm separates, resulting in four haploid daughter cells.
Chromatin vs. Chromatid
- Chromatin: Loose, thread-like form of DNA and proteins in a non-dividing cell; allows gene expression and DNA replication.
- Chromatid: Condensed, replicated form of DNA during cell division; two chromatids (sister chromatids) make up a chromosome, joined at the centromere.
Sister Chromatids vs. Homologous Chromosomes
- Sister Chromatids: Identical copies of a chromosome, joined at the centromere, created after DNA replication. Separated during meiosis II
- Homologous Chromosomes: Paired chromosomes, one from each parent, that have the same genes but may carry different alleles. They pair up during meiosis I.
Gamete Production and Sex Cells
- Spermatogenesis: Leads to the formation of sperm; Production of 4 functional sperm (haploid); Spermatogenesis begins at puberty and continues throughout life. Sperm produced in large numbers
- Oogenesis: Helps in the formation of ova; Production of 1 functional egg per cycle, and usually two or three polar bodies; is cyclic, releases one egg per menstrual cycle; cytoplasm is not divided equally, so that one egg has ample nutrients.
Abnormal Meiosis: Nondisjunction
- Occurs when two homologous chromosomes move to the same pole during meiosis
- Results in one daughter cell with an extra chromosome and one lacking a chromosome
- Can occur during mitosis or meiosis (mitosis has little effect; but meiosis affects fertilized egg), resulting in aneuploidy.
- Radiation increases incidence of nondisjunction.
Trisomy vs Monosomy
- Trisomy: Three chromosomes in the place of a normal pair (e.g., human with 47 chromosomes)
- Monosomy: Single chromosome in the place of a normal pair (e.g., human with 45 chromosomes)
- Polyploidy: More than two complete sets of chromosomes; common in plants
Nondisjunction Disorders
- Examples include Down syndrome (trisomy 21), Turner syndrome (monosomy X), and Klinefelter syndrome (XXY).
Mendelian Genetics
- Mendel: Austrian monk who discovered basic principles of heredity through pea plant experiments.
- Identified traits as inherited discrete units (genes).
- Discovered predictable patterns of inheritance.
Mendel's Laws
- Law of Segregation: Each individual has two alleles for a gene (one from each parent); alleles separate during gamete formation.
- Law of Independent Assortment: Different genes independently separate during gamete formation.
- Law of Dominance: When two different alleles for a gene are present, the dominant allele masks the recessive allele's expression.
Genotype vs. Phenotype
- Genotype: genetic makeup (e.g., 'Pp' for flower color)
- Phenotype: observable trait (e.g., 'purple' flowers for 'Pp' genotype).
Homozygous vs. Heterozygous
- Homozygous: two identical alleles (e.g., 'AA' or 'aa')
- Heterozygous: two different alleles (e.g., 'Aa')
Pedigree
- Charts to trace trait inheritance, predicting likelihood of genetic conditions and identifying carriers of recessive traits.
- Symbols used in pedigrees: circles for females, squares for males, shaded shapes= those who express the trait/ have the condition, unshaded shapes=do not express the trait
Incomplete Dominance vs. Codominance
- Incomplete Dominance: Heterozygote shows a blend of two alleles (e.g., pink flowers from red and white)
- Codominance: Both alleles are expressed equally (e.g., AB blood type with both A and B markers).
Sex-linked traits
- Traits located on the sex chromosomes (X and Y)
- Recessive traits on the X-chromosome are more common in males because males only have one X chromosome
- Females have to have two copies of a recessive allele to express the trait
Ethical issues in IVF
- Embryos are potential lives and it's unethical to experiment on them.
- Allowing adoption of embryos could influence the practice of creating more embryos than needed, which may conflict with natural principles for some viewpoints.
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Description
This quiz explores critical concepts in biology concerning DNA structure, cell division, and genetic inheritance. It also covers advanced topics such as cloning, genetic modification, and the implications of epigenetics. Test your understanding of these essential biological processes and their significance in multicellular organisms.