Biology Chapter: DNA and Cell Division
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Questions and Answers

What is the structure and function of DNA in eukaryotic cells?

DNA is a double-stranded molecule located in the nucleus that contains the genetic information necessary for producing proteins.

How do haploid and diploid cells differ in terms of chromosome number?

Haploid cells contain one complete set of chromosomes (23 in humans), while diploid cells contain two sets (46 in humans).

What are chromosomes and what role do they play in genetics?

Chromosomes are structures made of DNA and protein that organize genetic material, with humans having 46 chromosomes divided into 23 pairs.

What is the significance of the gene's role in inheritance?

<p>Genes are short sections of DNA that code for proteins, giving organisms their specific traits inherited from their parents.</p> Signup and view all the answers

Why is cell division important for multicellular organisms?

<p>Cell division is crucial for growth, repair, and maintenance of tissues in multicellular organisms.</p> Signup and view all the answers

What are two benefits of genetic modification in crops?

<p>Improved nutritional content and drought tolerance.</p> Signup and view all the answers

Describe the initial process used in cloning Dolly the sheep.

<p>The nucleus was taken from a mammary gland cell and placed into an empty egg cell, which was then stimulated to divide.</p> Signup and view all the answers

Why is cloning adult somatic cells more challenging than embryonic cells?

<p>Adult somatic cells are highly differentiated and require epigenetic reprogramming to revert to a pluripotent state.</p> Signup and view all the answers

What happens to the embryo after confirming normal development during Dolly's cloning process?

<p>The embryo is transferred into a surrogate mother.</p> Signup and view all the answers

What is differentiation in the context of cell biology?

<p>Differentiation is the process by which unspecialized stem cells become specialized in structure and function.</p> Signup and view all the answers

List one ethical issue associated with the use of in vitro fertilization.

<p>The potential for embryos to be experimented on raises ethical concerns regarding their status as potential lives.</p> Signup and view all the answers

How can biotechnology contribute to food security?

<p>Biotechnology can create drought-tolerant crops, ensuring food supplies in water-scarce areas.</p> Signup and view all the answers

What are the implications of epigenetic modifications in the cloning process?

<p>Epigenetic modifications in somatic cells can hinder the reprogramming necessary for successful cloning.</p> Signup and view all the answers

What are sister chromatids and how do they differ from homologous chromosomes?

<p>Sister chromatids are identical copies of a chromosome joined at the centromere, while homologous chromosomes are paired chromosomes from each parent with potentially different alleles.</p> Signup and view all the answers

What is a tetrad and when does it form during meiosis?

<p>A tetrad is a foursome of chromatids that forms when replicated homologous chromosomes align during meiosis I.</p> Signup and view all the answers

Describe the process of crossing over and its significance in genetic diversity.

<p>Crossing over is the exchange of DNA between paired homologous chromosomes during meiosis, increasing genetic diversity by creating new allele combinations.</p> Signup and view all the answers

What role do spindle fibers play during cell division?

<p>Spindle fibers help move and separate chromosomes during both mitosis and meiosis, ensuring proper distribution of genetic material to daughter cells.</p> Signup and view all the answers

How does spermatogenesis differ from oogenesis in terms of product and process?

<p>Spermatogenesis produces four functional sperm per cycle and continues throughout a male's life, while oogenesis typically produces one functional egg per cycle and is cyclic, occurring monthly.</p> Signup and view all the answers

What defines menopause and at what age does it typically occur?

<p>Menopause is defined as the cessation of menstrual cycles for at least 12 consecutive months and typically occurs between ages 45 and 55.</p> Signup and view all the answers

What are autosomes, and how do they differ from sex chromosomes?

<p>Autosomes are non-sex chromosomes that carry the majority of an organism's genetic information, unlike sex chromosomes which determine an organism's sex.</p> Signup and view all the answers

Explain the concept of segregation in meiosis and its importance.

<p>Segregation in meiosis is the separation of homologous chromosomes and their alleles into different gametes, ensuring genetic diversity among offspring.</p> Signup and view all the answers

What are alleles and how do they relate to traits in an organism?

<p>Alleles are different versions of a gene that determine specific traits. Each organism inherits two alleles for each gene, one from each parent.</p> Signup and view all the answers

Explain Mendel’s Law of Segregation.

<p>Mendel's Law of Segregation states that during gamete formation, the two alleles for a gene separate so that each gamete receives only one allele from each gene pair.</p> Signup and view all the answers

What does the term ‘homozygous’ mean in genetics?

<p>Homozygous refers to an organism that has two identical alleles for a particular gene, such as AA or aa.</p> Signup and view all the answers

Differentiate between genotype and phenotype.

<p>Genotype is the genetic makeup of an organism, representing the alleles it carries, while phenotype is the observable physical expression of a trait that results from the genotype.</p> Signup and view all the answers

What is a Punnett square and its purpose in genetics?

<p>A Punnett square is a tool used to predict the genetic outcomes and probabilities of different genotypes and phenotypes in offspring.</p> Signup and view all the answers

What do shaded and unshaded shapes represent in a pedigree?

<p>In a pedigree, shaded shapes indicate individuals who express a particular trait or condition, while unshaded shapes represent individuals who do not express the trait.</p> Signup and view all the answers

Describe Mendel’s Law of Independent Assortment.

<p>Mendel's Law of Independent Assortment states that different genes independently separate from one another when reproductive cells develop.</p> Signup and view all the answers

What does 'F1' signify in genetics?

<p>'F1' stands for the first filial generation, which refers to the first generation of offspring resulting from a cross between two parental organisms.</p> Signup and view all the answers

What is nondisjunction and what are its effects during meiosis?

<p>Nondisjunction is the failure of homologous chromosomes to separate during meiosis, resulting in one daughter cell with an extra chromosome and another with one less chromosome.</p> Signup and view all the answers

Define aneuploidy and give two examples.

<p>Aneuploidy is the condition of having an abnormal number of chromosomes. Examples include trisomy (e.g., Down syndrome with 47 chromosomes) and monosomy (e.g., Turner syndrome with 45 chromosomes).</p> Signup and view all the answers

Explain the role of maternal age in meiotic nondisjunction.

<p>Increased maternal age is associated with a higher incidence of meiotic nondisjunction, leading to conditions such as Down syndrome.</p> Signup and view all the answers

What is Turner syndrome and what are its characteristics?

<p>Turner syndrome is a condition resulting from the monosomy of sex chromosomes, specifically having one X chromosome (XO). Characteristics include short stature, thick necks, and underdeveloped sexual features.</p> Signup and view all the answers

Describe Klinefelter syndrome and its possible effects on individuals.

<p>Klinefelter syndrome occurs in individuals with an extra X chromosome (XXY), leading to lower testosterone levels and a feminized appearance. Affected individuals may experience infertility and language problems.</p> Signup and view all the answers

What is the significance of a karyotype in genetics?

<p>A karyotype is the complete set of chromosomes in an individual, used to determine if there is a normal set of 46 chromosomes or identify chromosomal abnormalities.</p> Signup and view all the answers

How does polyploidy differ from nondisjunction, and in which organisms is it common?

<p>Polyploidy involves having more than two complete sets of chromosomes, while nondisjunction leads to an abnormal number of individual chromosomes. It is common in plants such as wheat and bananas.</p> Signup and view all the answers

What are the effects of nondisjunction during mitosis compared to meiosis?

<p>Nondisjunction during mitosis usually has minimal effect, affecting only one cell, while in meiosis, it can lead to significant genetic disorders in gametes that may result in multi-celled organisms.</p> Signup and view all the answers

What is the role of the Barr body in female mammals?

<p>The Barr body represents the inactivated X chromosome, helping to balance gene expression between males and females by preventing excess gene activity from X-linked genes.</p> Signup and view all the answers

Why are fruit flies commonly used in genetic experiments?

<p>Fruit flies are favored for genetic studies due to their short life cycle, large number of offspring, simple genetic makeup, observable traits, and low cost.</p> Signup and view all the answers

How does artificial insemination differ from in vitro fertilization?

<p>In artificial insemination, sperm is placed in the female's reproductive tract without sexual intercourse, whereas in vitro fertilization involves fertilizing egg cells outside the body and then transferring the embryo to the uterus.</p> Signup and view all the answers

What defines recombinant DNA, and can you provide an example?

<p>Recombinant DNA is created by combining genetic material from different sources; for example, BT corn contains a gene from a soil bacterium that makes it resistant to corn borers.</p> Signup and view all the answers

Why is plant cloning generally easier than animal cloning?

<p>Plant cloning is simpler because plants can regenerate from root or stem cuttings, while animal cloning is complex and often fails due to specialized cell types that cannot easily regenerate.</p> Signup and view all the answers

What are two reasons for using biotechnology in agriculture?

<p>Biotechnology in agriculture can increase crop yields and create pest and disease-resistant crops, which reduces the necessity for chemical pesticides.</p> Signup and view all the answers

What is gene therapy and its purpose?

<p>Gene therapy involves inserting genes into an individual's cells to treat genetic diseases, aiming to correct or replace malfunctioning genes.</p> Signup and view all the answers

Why might the success rate of animal cloning be lower than that of plant cloning?

<p>Animal cloning has a lower success rate because it often requires specialized cells to be reprogrammed, which can have permanently turned off genes, making the process complex.</p> Signup and view all the answers

Study Notes

DNA

  • Located in the nucleus of eukaryotes
  • Contains genetic information
  • Double-stranded and twisted into a double helix

Gene

  • Short section of DNA
  • Used to produce proteins
  • Determines traits

Chromosomes

  • Structures organizing genetic material
  • Made of DNA and protein
  • One unbroken DNA molecule forms each chromosome
  • Humans have 46 chromosomes (23 pairs)
  • 22 pairs are autosomes, 1 pair are sex chromosomes (e.g., X and Y)
  • Females are XX, males are XY

Haploid vs. Diploid

Haploid (n)

  • Contains one complete set of chromosomes (n)
  • In humans, haploid cells have 23 chromosomes (one from each pair)
  • Found in gametes (sperm and egg cells)
  • Formed through meiosis, reducing the chromosome number by half

Diploid (2n)

  • Contains two complete sets of chromosomes (2n)
  • In humans, diploid cells have 46 chromosomes (23 pairs)
  • One chromosome of each pair is inherited from each parent
  • Found in most body cells (somatic cells)
  • Formed through mitosis

Meiosis

  • Process forming gametes (sex cells)
  • Involves two stages of cell division resulting in cells with half the number of chromosomes

Meiosis 1

  • Prophase 1: Nuclear membrane dissolves, chromosomes condense, homologous chromosomes come together (synapse) to form a tetrad, crossing over occurs
  • Metaphase 1: Chromosome pairs line up side by side at the equatorial plate
  • Anaphase 1: Each chromosome separates from its homologue (pair), moving to opposite poles, the chromosomes do not separate at the centromere
  • Telophase 1: Nucleus reforms around chromosomes, cytoplasm divides (cytokinesis occurs).
  • Resulting in two haploid daughter cells.

Meiosis 2

  • Very similar to mitosis (but with half the number of chromosomes)
  • Prophase 2: Nuclear membrane dissolves
  • Metaphase 2: Chromosomes line up along equatorial plate
  • Anaphase 2: Sister chromatids are separated and move to opposite poles
  • Telophase 2: Nucleus reforms, Cytoplasm separates, resulting in four haploid daughter cells.

Chromatin vs. Chromatid

  • Chromatin: Loose, thread-like form of DNA and proteins in a non-dividing cell; allows gene expression and DNA replication.
  • Chromatid: Condensed, replicated form of DNA during cell division; two chromatids (sister chromatids) make up a chromosome, joined at the centromere.

Sister Chromatids vs. Homologous Chromosomes

  • Sister Chromatids: Identical copies of a chromosome, joined at the centromere, created after DNA replication. Separated during meiosis II
  • Homologous Chromosomes: Paired chromosomes, one from each parent, that have the same genes but may carry different alleles. They pair up during meiosis I.

Gamete Production and Sex Cells

  • Spermatogenesis: Leads to the formation of sperm; Production of 4 functional sperm (haploid); Spermatogenesis begins at puberty and continues throughout life. Sperm produced in large numbers
  • Oogenesis: Helps in the formation of ova; Production of 1 functional egg per cycle, and usually two or three polar bodies; is cyclic, releases one egg per menstrual cycle; cytoplasm is not divided equally, so that one egg has ample nutrients.

Abnormal Meiosis: Nondisjunction

  • Occurs when two homologous chromosomes move to the same pole during meiosis
  • Results in one daughter cell with an extra chromosome and one lacking a chromosome
  • Can occur during mitosis or meiosis (mitosis has little effect; but meiosis affects fertilized egg), resulting in aneuploidy.
  • Radiation increases incidence of nondisjunction.

Trisomy vs Monosomy

  • Trisomy: Three chromosomes in the place of a normal pair (e.g., human with 47 chromosomes)
  • Monosomy: Single chromosome in the place of a normal pair (e.g., human with 45 chromosomes)
  • Polyploidy: More than two complete sets of chromosomes; common in plants

Nondisjunction Disorders

  • Examples include Down syndrome (trisomy 21), Turner syndrome (monosomy X), and Klinefelter syndrome (XXY).

Mendelian Genetics

  • Mendel: Austrian monk who discovered basic principles of heredity through pea plant experiments.
  • Identified traits as inherited discrete units (genes).
  • Discovered predictable patterns of inheritance.

Mendel's Laws

  • Law of Segregation: Each individual has two alleles for a gene (one from each parent); alleles separate during gamete formation.
  • Law of Independent Assortment: Different genes independently separate during gamete formation.
  • Law of Dominance: When two different alleles for a gene are present, the dominant allele masks the recessive allele's expression.

Genotype vs. Phenotype

  • Genotype: genetic makeup (e.g., 'Pp' for flower color)
  • Phenotype: observable trait (e.g., 'purple' flowers for 'Pp' genotype).

Homozygous vs. Heterozygous

  • Homozygous: two identical alleles (e.g., 'AA' or 'aa')
  • Heterozygous: two different alleles (e.g., 'Aa')

Pedigree

  • Charts to trace trait inheritance, predicting likelihood of genetic conditions and identifying carriers of recessive traits.
  • Symbols used in pedigrees: circles for females, squares for males, shaded shapes= those who express the trait/ have the condition, unshaded shapes=do not express the trait

Incomplete Dominance vs. Codominance

  • Incomplete Dominance: Heterozygote shows a blend of two alleles (e.g., pink flowers from red and white)
  • Codominance: Both alleles are expressed equally (e.g., AB blood type with both A and B markers).

Sex-linked traits

  • Traits located on the sex chromosomes (X and Y)
  • Recessive traits on the X-chromosome are more common in males because males only have one X chromosome
  • Females have to have two copies of a recessive allele to express the trait

Ethical issues in IVF

  • Embryos are potential lives and it's unethical to experiment on them.
  • Allowing adoption of embryos could influence the practice of creating more embryos than needed, which may conflict with natural principles for some viewpoints.

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Description

This quiz explores critical concepts in biology concerning DNA structure, cell division, and genetic inheritance. It also covers advanced topics such as cloning, genetic modification, and the implications of epigenetics. Test your understanding of these essential biological processes and their significance in multicellular organisms.

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